Bestrophin Antibody - #DF13298

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Product: Bestrophin Antibody
Catalog: DF13298
Description: Rabbit polyclonal antibody to Bestrophin
Application: WB
Reactivity: Human, Mouse, Rat, Bovine
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 67kDa; 68kD(Calculated).
Uniprot: O76090
RRID: AB_2846317

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Bovine
Prediction:
Horse(92%), Sheep(100%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
Bestrophin Antibody detects endogenous levels of total Bestrophin.
RRID:
AB_2846317
Cite Format: Affinity Biosciences Cat# DF13298, RRID:AB_2846317.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARB; BEST 1; BEST; Best disease; Best macular dystrophy; BEST1; BEST1_HUMAN; Best1V1Delta2; Bestrophin 1; Bestrophin-1; Bestrophin1; BMD; mBest1; RP50; TU15B; Vitelliform macular dystrophy 2; Vitelliform macular dystrophy; Vitelliform macular dystrophy protein 2; VMD 2; VMD2;

Immunogens

Immunogen:
Uniprot:

O76090(BEST1_HUMAN) >>Visit HPA database.

Gene(ID):
BEST1(7439)
Expression:
O76090 BEST1_HUMAN:

Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.

Sequence:
MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRDEAHS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Horse
92
Dog
92
Rabbit
92
Xenopus
75
Chicken
67
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O76090 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation
S16 Phosphorylation
Y148 Phosphorylation
Y227 Phosphorylation
S358 Phosphorylation P17252 (PRKCA) , Q05655 (PRKCD)
T504 Phosphorylation
K511 Ubiquitination
T536 Phosphorylation

Research Backgrounds

Function:

Forms calcium-sensitive chloride channels. Highly permeable to bicarbonate.

PTMs:

Phosphorylated by PP2A.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Basolateral cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.

Subunit Structure:

Homooligomer (tetramer or pentamer). May interact with PPP2CB and PPP2R1B (By similarity).

Family&Domains:

Belongs to the bestrophin family.

Restrictive clause

 

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