Smoothelin Antibody - #DF13304

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Product: Smoothelin Antibody
Catalog: DF13304
Description: Rabbit polyclonal antibody to Smoothelin
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 99kDa; 99kD(Calculated).
Uniprot: P53814
RRID: AB_2846323

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Smoothelin Antibody detects endogenous levels of total Smoothelin.
RRID:
AB_2846323
Cite Format: Affinity Biosciences Cat# DF13304, RRID:AB_2846323.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ35168; FLJ35365; FLJ35620; FLJ38597; Smoothelin; SMSMO; Smtn; SMTN_HUMAN;

Immunogens

Immunogen:
Uniprot:

P53814(SMTN_HUMAN) >>Visit HPA database.

Gene(ID):
SMTN(6525)
Expression:
P53814 SMTN_HUMAN:

Smooth muscle; contractile or vascular (for the long form).

Sequence:
MADEALAGLDEGALRKLLEVTADLAERRRIRSAIRELQRQELEREEEALASKRFRAERQDNKENWLHSQQREAEQRAALARLAGQLESMNDVEELTALLRSAGEYEERKLIRAAIRRVRAQEIEAATLAGRLYSGRPNSGSREDSKGLAAHRLEQCEVPEREEQEQQAEVSKPTPTPEGTSQDVTTVTLLLRAPPGSTSSSPASPSSSPTPASPEPPLEPAEAQCLTAEVPGSPEPPPSPPKTTSPEPQESPTLPSTEGQVVNKLLSGPKETPAAQSPTRGPSDTKRADVAGPRPCQRSLSVLSPRQPAQNRESTPLASGPSSFQRAGSVRDRVHKFTSDSPMAARLQDGTPQAALSPLTPARLLGPSLTSTTPASSSSGSSSRGPSDTSSRFSKEQRGVAQPLAQLRSCPQEEGPRGRGLAARPLENRAGGPVARSEEPGAPLPVAVGTAEPGGSMKTTFTIEIKDGRGQASTGRVLLPTGNQRAELTLGLRAPPTLLSTSSGGKSTITRVNSPGTLARLGSVTHVTSFSHAPPSSRGGCSIKMEAEPAEPLAAAVEAANGAEQTRVNKAPEGRSPLSAEELMTIEDEGVLDKMLDQSTDFEERKLIRAALRELRQRKRDQRDKERERRLQEARGRPGEGRGNTATETTTRHSQRAADGSAVSTVTKTERLVHSNDGTRTARTTTVESSFVRRSENGSGSTMMQTKTFSSSSSSKKMGSIFDREDQASPRAGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGAAGSPGGPRAAVQRSTSFGVPNANSIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEVAFSSAEMLVDCVPLVEVDDMMIMGKKPDPKCVFTYVQSLYNHLRRHELRLRGKNV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
75
Chicken
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53814 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K16 Ubiquitination
S88 Phosphorylation
S139 Phosphorylation
T243 Phosphorylation
T244 Phosphorylation
S245 Phosphorylation
S251 Phosphorylation
S267 Phosphorylation
T272 Phosphorylation
S277 Phosphorylation
T279 Phosphorylation
S299 Phosphorylation
S301 Phosphorylation
S304 Phosphorylation
T315 Phosphorylation
S319 Phosphorylation
S322 Phosphorylation
S323 Phosphorylation
K336 Methylation
T338 Phosphorylation
S339 Phosphorylation
S341 Phosphorylation
T351 Phosphorylation
S357 Phosphorylation
T360 Phosphorylation
S371 Phosphorylation
T372 Phosphorylation
T373 Phosphorylation
S376 Phosphorylation
S381 Phosphorylation
S382 Phosphorylation
R384 Methylation
S391 Phosphorylation
S394 Phosphorylation
R419 Methylation
R424 Methylation
R436 Methylation
T481 Phosphorylation
S503 Phosphorylation
K506 Ubiquitination
S514 Phosphorylation
S523 Phosphorylation
T525 Phosphorylation
R538 Methylation
S576 Phosphorylation
S579 Phosphorylation
T585 Phosphorylation
K668 Sumoylation
T684 Phosphorylation
S690 Phosphorylation
S695 Phosphorylation
K716 Acetylation
S720 Phosphorylation
S729 Phosphorylation
S734 Phosphorylation
K740 Ubiquitination
S754 Phosphorylation
K757 Acetylation
K765 Acetylation
K765 Methylation
S779 Phosphorylation
T791 Phosphorylation
S792 Phosphorylation
S800 Phosphorylation

Research Backgrounds

Function:

Structural protein of the cytoskeleton.

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: Exhibits a filamentous organization.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Smooth muscle; contractile or vascular (for the long form).

Family&Domains:

Belongs to the smoothelin family.

References

1). ZBTB16 drives vascular calcification through accelerating VSMCs osteoblastic transition in chronic kidney disease via Wnt/β-catenin pathway. Shock (Augusta, Ga.), 2024 (PubMed: 39450908) [IF=3.1]

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