CHL1 Antibody - #DF13313
| Product: | CHL1 Antibody |
| Catalog: | DF13313 |
| Description: | Rabbit polyclonal antibody to CHL1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog, Chicken |
| Mol.Wt.: | 135kDa; 135kD(Calculated). |
| Uniprot: | O00533 |
| RRID: | AB_2846332 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13313, RRID:AB_2846332.
Fold/Unfold
CALL; Cell adhesion molecule with homology to L1CAM (close homolog of L1); Cell adhesion molecule with homology to L1CAM (close homologue of L1); Cell adhesion molecule with homology to L1CAM; Close homolog of L1; FLJ44930; L1 cell adhesion molecule 2; L1CAM2; MGC132578; Neural cell adhesion molecule; Neural cell adhesion molecule L1-like protein; CHL1;
Immunogens
Expressed in the fetal and adult brain as well as in Schwann cell culture. Also detected in adult peripheral tissues.
- O00533 NCHL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPLLLGRGLIVYLMFLLLKFSKAIEIPSSVQQVPTIIKQSKVQVAFPFDEYFQIECEAKGNPEPTFSWTKDGNPFYFTDHRIIPSNNSGTFRIPNEGHISHFQGKYRCFASNKLGIAMSEEIEFIVPSVPKFPKEKIDPLEVEEGDPIVLPCNPPKGLPPLHIYWMNIELEHIEQDERVYMSQKGDLYFANVEEKDSRNDYCCFAAFPRLRTIVQKMPMKLTVNSSNSIKQRKPKLLLPPTESGSESSITILKGEILLLECFAEGLPTPQVDWNKIGGDLPKGRETKENYGKTLKIENVSYQDKGNYRCTASNFLGTATHDFHVIVEEPPRWTKKPQSAVYSTGSNGILLCEAEGEPQPTIKWRVNGSPVDNHPFAGDVVFPREISFTNLQPNHTAVYQCEASNVHGTILANANIDVVDVRPLIQTKDGENYATVVGYSAFLHCEFFASPEAVVSWQKVEEVKPLEGRRYHIYENGTLQINRTTEEDAGSYSCWVENAIGKTAVTANLDIRNATKLRVSPKNPRIPKLHMLELHCESKCDSHLKHSLKLSWSKDGEAFEINGTEDGRIIIDGANLTISNVTLEDQGIYCCSAHTALDSAADITQVTVLDVPDPPENLHLSERQNRSVRLTWEAGADHNSNISEYIVEFEGNKEEPGRWEELTRVQGKKTTVILPLAPFVRYQFRVIAVNEVGRSQPSQPSDHHETPPAAPDRNPQNIRVQASQPKEMIIKWEPLKSMEQNGPGLEYRVTWKPQGAPVEWEEETVTNHTLRVMTPAVYAPYDVKVQAINQLGSGPDPQSVTLYSGEDYPDTAPVIHGVDVINSTLVKVTWSTVPKDRVHGRLKGYQINWWKTKSLLDGRTHPKEVNILRFSGQRNSGMVPSLDAFSEFHLTVLAYNSKGAGPESEPYIFQTPEGVPEQPTFLKVIKVDKDTATLSWGLPKKLNGNLTGYLLQYQIINDTYEIGELNDINITTPSKPSWHLSNLNATTKYKFYLRACTSQGCGKPITEESSTLGEGSKGIGKISGVNLTQKTHPIEVFEPGAEHIVRLMTKNWGDNDSIFQDVIETRGREYAGLYDDISTQGWFIGLMCAIALLTLLLLTVCFVKRNRGGKYSVKEKEDLHPDPEIQSVKDETFGEYSDSDEKPLKGSLRSLNRDMQPTESADSLVEYGEGDHGLFSEDGSFIGAYAGSKEKGSVESNGSSTATFPLRA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O00533 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N476 | N-Glycosylation | Uniprot | |
| N482 | N-Glycosylation | Uniprot | |
| N562 | N-Glycosylation | Uniprot | |
| Y747 | Phosphorylation | Uniprot | |
| N767 | N-Glycosylation | Uniprot | |
| Y778 | Phosphorylation | Uniprot | |
| N822 | N-Glycosylation | Uniprot | |
| S871 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| T933 | Phosphorylation | Uniprot | |
| S935 | Phosphorylation | Uniprot | |
| Y953 | Phosphorylation | Uniprot | |
| T971 | Phosphorylation | Uniprot | |
| N1026 | N-Glycosylation | Uniprot | |
| K1110 | Ubiquitination | Uniprot | |
| S1112 | Phosphorylation | Uniprot | |
| S1127 | Phosphorylation | Uniprot | |
| S1137 | Phosphorylation | Uniprot | |
| S1147 | Phosphorylation | Uniprot | |
| S1150 | Phosphorylation | Uniprot | |
| S1180 | Phosphorylation | Uniprot | |
| S1196 | Phosphorylation | Uniprot | |
| S1199 | Phosphorylation | Uniprot |
Research Backgrounds
Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane (By similarity).
Cleavage by metalloprotease ADAM8 in the extracellular part generates 2 soluble forms (125 kDa and 165 kDa) in vitro and is inhibited by metalloprotease inhibitors (By similarity). Cleaved by BACE1 (By similarity).
N-glycosylated. Contains N-linked oligosaccharides with a sulfated carbohydrate structure type HNK-1 (SO4-3-GlcUABeta1,3GalBeta1,4GlcNAc) (By similarity).
O-glycosylated.
Cell membrane>Single-pass type I membrane protein.
Note: Soluble forms produced by cleavage/shedding also exist.
Secreted>Extracellular space>Extracellular matrix.
Expressed in the fetal and adult brain as well as in Schwann cell culture. Also detected in adult peripheral tissues.
May interact with L1CAM. May interact with ITGB1/ITGA1 heterodimer and ITGB1/ITGA2 heterodimer as well as with ANK3 (By similarity).
The FIG[AQ]Y motif seems to be an ankyrin recruitment region.
The DGEA motif seems to be a recognition site for integrin.
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
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