SMC2 Antibody - #DF13315

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Product: SMC2 Antibody
Catalog: DF13315
Description: Rabbit polyclonal antibody to SMC2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 135kDa; 136kD(Calculated).
Uniprot: O95347
RRID: AB_2846334

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Zebrafish(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SMC2 Antibody detects endogenous levels of total SMC2.
RRID:
AB_2846334
Cite Format: Affinity Biosciences Cat# DF13315, RRID:AB_2846334.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAP E; CAPE; Chromosome associated protein E; Chromosome-associated protein E; FLJ10093; hCAP E; hCAP-E; hCAPE; PRO0324; SMC 2; SMC protein 2; SMC-2; SMC2; SMC2 L1; SMC2_HUMAN; SMC2L1; Structural maintenance of chromosomes (SMC) family member chromosome associated protein E; Structural maintenance of chromosomes 2; Structural maintenance of chromosomes 2 like 1; Structural maintenance of chromosomes 2 yeast like 1; Structural maintenance of chromosomes protein 2; XCAP E homolog; XCAP-E homolog; XCAPE homolog;

Immunogens

Immunogen:
Uniprot:

O95347(SMC2_HUMAN) >>Visit HPA database.

Gene(ID):
SMC2(10592)
Sequence:
MHIKSIILEGFKSYAQRTEVNGFDPLFNAITGLNGSGKSNILDSICFLLGISNLSQVRASNLQDLVYKNGQAGITKASVSITFDNSDKKQSPLGFEVHDEITVTRQVVIGGRNKYLINGVNANNTRVQDLFCSVGLNVNNPHFLIMQGRITKVLNMKPPEILSMIEEAAGTRMYEYKKIAAQKTIEKKEAKLKEIKTILEEEITPTIQKLKEERSSYLEYQKVMREIEHLSRLYIAYQFLLAEDTKVRSAEELKEMQDKVIKLQEELSENDKKIKALNHEIEELEKRKDKETGGILRSLEDALAEAQRVNTKSQSAFDLKKKNLACEESKRKELEKNMVEDSKTLAAKEKEVKKITDGLHALQEASNKDAEALAAAQQHFNAVSAGLSSNEDGAEATLAGQMMACKNDISKAQTEAKQAQMKLKHAQQELKNKQAEVKKMDSGYRKDQEALEAVKRLKEKLEAEMKKLNYEENKEESLLEKRRQLSRDIGRLKETYEALLARFPNLRFAYKDPEKNWNRNCVKGLVASLISVKDTSATTALELVAGERLYNVVVDTEVTGKKLLERGELKRRYTIIPLNKISARCIAPETLRVAQNLVGPDNVHVALSLVEYKPELQKAMEFVFGTTFVCDNMDNAKKVAFDKRIMTRTVTLGGDVFDPHGTLSGGARSQAASILTKFQELKDVQDELRIKENELRALEEELAGLKNTAEKYRQLKQQWEMKTEEADLLQTKLQQSSYHKQQEELDALKKTIEESEETLKNTKEIQRKAEEKYEVLENKMKNAEAERERELKDAQKKLDCAKTKADASSKKMKEKQQEVEAITLELEELKREHTSYKQQLEAVNEAIKSYESQIEVMAAEVAKNKESVNKAQEEVTKQKEVITAQDTVIKAKYAEVAKHKEQNNDSQLKIKELDHNISKHKREAEDGAAKVSKMLKDYDWINAERHLFGQPNSAYDFKTNNPKEAGQRLQKLQEMKEKLGRNVNMRAMNVLTEAEERYNDLMKKKRIVENDKSKILTTIEDLDQKKNQALNIAWQKVNKDFGSIFSTLLPGANAMLAPPEGQTVLDGLEFKVALGNTWKENLTELSGGQRSLVALSLILSMLLFKPAPIYILDEVDAALDLSHTQNIGQMLRTHFTHSQFIVVSLKEGMFNNANVLFKTKFVDGVSTVARFTQCQNGKISKEAKSKAKPPKGAHVEV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Xenopus
100
Zebrafish
100
Pig
88
Bovine
88
Sheep
88
Rabbit
88
Chicken
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95347 As Substrate

Site PTM Type Enzyme
K4 Ubiquitination
K12 Ubiquitination
S60 Phosphorylation
Y67 Phosphorylation
K68 Ubiquitination
K89 Ubiquitination
K114 Acetylation
K114 Ubiquitination
K152 Ubiquitination
K157 Ubiquitination
K183 Ubiquitination
K196 Ubiquitination
K209 Ubiquitination
K211 Ubiquitination
K222 Acetylation
K222 Ubiquitination
S249 Phosphorylation
S268 Phosphorylation
K275 Ubiquitination
K290 Ubiquitination
T292 Phosphorylation
K312 Ubiquitination
K320 Acetylation
K321 Acetylation
K330 Acetylation
K336 Acetylation
K348 Acetylation
K353 Acetylation
S477 Phosphorylation
K481 Ubiquitination
S486 Phosphorylation
K493 Ubiquitination
K511 Ubiquitination
K515 Ubiquitination
K523 Ubiquitination
K533 Ubiquitination
K562 Ubiquitination
T574 Phosphorylation
K580 Ubiquitination
C585 S-Nitrosylation
K677 Acetylation
K677 Ubiquitination
K682 Ubiquitination
K691 Ubiquitination
K706 Ubiquitination
K711 Ubiquitination
K732 Ubiquitination
K768 Acetylation
K792 Acetylation
K813 Acetylation
K837 Acetylation
K911 Ubiquitination
K936 Ubiquitination
K958 Acetylation
K958 Ubiquitination
K963 Acetylation
K971 Acetylation
K971 Ubiquitination
T992 Phosphorylation
K1003 Ubiquitination
K1014 Ubiquitination
T1017 Phosphorylation
T1018 Phosphorylation
K1025 Ubiquitination
K1026 Ubiquitination
K1036 Ubiquitination
K1039 Ubiquitination
K1079 Ubiquitination
K1158 Acetylation
K1158 Methylation
K1158 Ubiquitination
T1159 Phosphorylation
K1160 Acetylation
K1160 Methylation
K1160 Ubiquitination
K1178 Ubiquitination
K1188 Ubiquitination

Research Backgrounds

Function:

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

Subcellular Location:

Nucleus. Cytoplasm. Chromosome.
Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG. Interacts with BRD4 (isoform B), leading to insulate chromatin from DNA damage response pathway.

Family&Domains:

The SMC hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.

Belongs to the SMC family. SMC2 subfamily.

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