Lubricin Antibody | Affinity Biosciences

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Lubricin Antibody - Western blot analysis of extracts from Human liver , using Lubricin Antibody.

Lubricin Antibody - DF13331 at 1/100 staining Mouse muscle tissue by IHC-P.

Product:	Lubricin Antibody
Catalog:	DF13331
Description:	Rabbit polyclonal antibody to Lubricin
Application:	WB IHC
Cited expt.:	IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Dog
Mol.Wt.:	151kDa; 151kD(Calculated).
Uniprot:	Q92954
RRID:	AB_2846350

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Lubricin Antibody detects endogenous levels of total Lubricin.

RRID:

AB_2846350
Cite Format: Affinity Biosciences Cat# DF13331, RRID:AB_2846350.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Articular superficial zone protein; bG174L6.2; CACP; Camptodactyly arthropathy coxa vara pericarditis syndrome gene; FLJ32635; HAPO; Jacobs camptodactyly arthropathy pericarditis syndrome gene; JCAP; Megakaryocyte stimulating factor; MSF; PRG 4; PRG4; Proteoglycan 4; Proteoglycan4; Superficial zone proteoglycan; SZP;

Immunogens

Immunogen:

A synthesized peptide derived from human Lubricin, corresponding to a region within C-terminal amino acids.

Uniprot:

Q92954(PRG4_HUMAN) >>Visit HPA database.

Gene(ID):

PRG4(10216)

Expression:

Q92954 PRG4_HUMAN:

Highly expressed in synovial tissue, cartilage and liver and weakly in heart and lung. Isoform B is expressed in kidney, lung, liver, heart and brain. Isoform C and isoform D are widely expressed.

Sequence:

Q92954 PRG4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Sheep

100

Dog

100

Pig

Bovine

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q92954_PRG4_HUMAN

Function:

Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.

Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages.

PTMs:

N-glycosylated.

O-glycosylated; contains glycosaminoglycan chondroitin sulfate and keratan sulfate. O-glycosylated with sialylated oligosaccharides which are predominantly represented by the monosialylated core type I structure, NeuNAcalpha2-3Galbeta1-3GalNAc, with smaller amounts of disialylated O-glycans.

The disulfide bond between Cys-1146 and Cys-1403 is essential for protein cleavage.

Subcellular Location:

Secreted.

Tissue Specificity:

Highly expressed in synovial tissue, cartilage and liver and weakly in heart and lung. Isoform B is expressed in kidney, lung, liver, heart and brain. Isoform C and isoform D are widely expressed.

References

1). HAMA-SBMA hydrogel with anti-inflammatory properties delivers cartilage organoids, boosting cartilage regeneration. Journal of nanobiotechnology, 2025 (PubMed: 40448111) [IF=10.2]

Application: IHC Species: Rat Sample:

Fig. 1 CCO exhibits characteristics of articular cartilage and delays the process of ossification. (A) SO, staining of the CEP. CEP had a quasi-spherical shape. (B) DAPI staining. CEP decellularization of nuclei. (C) DNA content, GAG content, and Total collagen content measurement. CEP was decellularized, preserving a significant amount of GAG and collagen. (D) Cell viability and cytotoxicity assay using Calcein/PI at D1, D3, and D7, with live cells shown in green and dead cells in red. CEP exhibits good biocompatibility. (E) CCK-8 assay at D1, D3, and D7. CEP can promote the proliferation of BMSCs. (F) HE and AB staining of CO and CCO on days 7, 14, and 21. CCO can generate a greater amount of cartilage matrix. (G) Immunofluorescence images for COL2, ACAN in CO and CCO at days 14, with blue representing DAPI and red representing the target proteins. The cartilage markers COL2 and ACAN are expressed at higher levels in CCO. (H)Immunohistochemical images for PRG4 in CO and CCO at days 14 and Immunofluorescence images for COLX in CO and CCO at days 21 with blue representing DAPI and red representing the target proteins. CCO exhibits high expressions of the articular cartilage-specific marker PRG4 and low expressions of the hypertrophic cartilage marker COLX. (I) The mRNA expression of cartilage genes SOX9, COL2, and ACAN, as well as the hypertrophic cartilage gene COLX, was assessed in CO and CCO at 0, 7, 14, and 21 days. Both CCO and CO reached peak cartilage expression in 14 days, after which the expression of hypertrophy-related genes increased. However, CCO demonstrated superior cartilage formation compared to CO, with a delayed hypertrophic process. All experiments were repeated three times. ns p > 0.05, *0.01

2). PRG4 mitigates hemorrhagic shock-induced cardiac injury by inhibiting mitochondrial dysregulation, oxidative stress and NLRP3-mediated pyroptosis. International immunopharmacology, 2024 (PubMed: 38897120) [IF=4.8]

3). Punicalagin attenuates osteoarthritis progression via regulating Foxo1/Prg4/HIF3α axis. Bone, 2021 (PubMed: 34171516) [IF=3.5]

4). UBE2I regulates the nuclear translocation of hnRNPA2B1 by contributing to SUMO modification in osteoarthritis. Gene, 2024 (PubMed: 38955308) [IF=2.6]

5). Spatial distribution proteoglycans articular cartilage of femoral condyles and tibial plateau in experimental osteoarthritis in rats. Journal of Volgograd State Medical University, 2023

6). Пространственное распределение протеогликанов суставного хряща бедренных мыщелков и тибиального плато при экспериментальном остеоартрозе у крыс.
Научно-практический рецензируемый журнал

Клиническая и экспериментальная морфология
, 2024

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Lubricin Antibody - #DF13331