Lubricin Antibody - #DF13331
(3)
(2)
| Product: | Lubricin Antibody |
| Catalog: | DF13331 |
| Description: | Rabbit polyclonal antibody to Lubricin |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Sheep, Dog |
| Mol.Wt.: | 151kDa; 151kD(Calculated). |
| Uniprot: | Q92954 |
| RRID: | AB_2846350 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Lubricin Antibody detects endogenous levels of total Lubricin.
RRID:
AB_2846350
Cite Format: Affinity Biosciences Cat# DF13331, RRID:AB_2846350.
Cite Format: Affinity Biosciences Cat# DF13331, RRID:AB_2846350.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Articular superficial zone protein; bG174L6.2; CACP; Camptodactyly arthropathy coxa vara pericarditis syndrome gene; FLJ32635; HAPO; Jacobs camptodactyly arthropathy pericarditis syndrome gene; JCAP; Megakaryocyte stimulating factor; MSF; PRG 4; PRG4; Proteoglycan 4; Proteoglycan4; Superficial zone proteoglycan; SZP;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Q92954 PRG4_HUMAN:
Highly expressed in synovial tissue, cartilage and liver and weakly in heart and lung. Isoform B is expressed in kidney, lung, liver, heart and brain. Isoform C and isoform D are widely expressed.
Sequence:
- Q92954 PRG4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAWKTLPIYLLLLLSVFVIQQVSSQDLSSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTAELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPTSPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRKIKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTSDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Sheep
100
Dog
100
Pig
88
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q92954 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S24 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| Y100 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| S123 | O-Glycosylation | Uniprot | |
| S136 | O-Glycosylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| T240 | O-Glycosylation | Uniprot | |
| T253 | O-Glycosylation | Uniprot | |
| T277 | O-Glycosylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| T283 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| T291 | O-Glycosylation | Uniprot | |
| T305 | O-Glycosylation | Uniprot | |
| S306 | O-Glycosylation | Uniprot | |
| T310 | O-Glycosylation | Uniprot | |
| S317 | O-Glycosylation | Uniprot | |
| T324 | O-Glycosylation | Uniprot | |
| T332 | O-Glycosylation | Uniprot | |
| T338 | O-Glycosylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| T346 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| T367 | O-Glycosylation | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| S373 | O-Glycosylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| T376 | O-Glycosylation | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| T384 | O-Glycosylation | Uniprot | |
| T384 | Phosphorylation | Uniprot | |
| T385 | O-Glycosylation | Uniprot | |
| T386 | Phosphorylation | Uniprot | |
| S388 | O-Glycosylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| T391 | O-Glycosylation | Uniprot | |
| T399 | O-Glycosylation | Uniprot | |
| T400 | O-Glycosylation | Uniprot | |
| T407 | O-Glycosylation | Uniprot | |
| T408 | O-Glycosylation | Uniprot | |
| T415 | O-Glycosylation | Uniprot | |
| T423 | O-Glycosylation | Uniprot | |
| S427 | O-Glycosylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| T430 | O-Glycosylation | Uniprot | |
| T430 | Phosphorylation | Uniprot | |
| T438 | O-Glycosylation | Uniprot | |
| T438 | Phosphorylation | Uniprot | |
| T439 | O-Glycosylation | Uniprot | |
| T439 | Phosphorylation | Uniprot | |
| T446 | O-Glycosylation | Uniprot | |
| T447 | O-Glycosylation | Uniprot | |
| T454 | O-Glycosylation | Uniprot | |
| T455 | O-Glycosylation | Uniprot | |
| T462 | Phosphorylation | Uniprot | |
| T477 | O-Glycosylation | Uniprot | |
| T478 | O-Glycosylation | Uniprot | |
| T485 | O-Glycosylation | Uniprot | |
| T493 | O-Glycosylation | Uniprot | |
| T493 | Phosphorylation | Uniprot | |
| T494 | O-Glycosylation | Uniprot | |
| T501 | O-Glycosylation | Uniprot | |
| T501 | Phosphorylation | Uniprot | |
| T502 | O-Glycosylation | Uniprot | |
| K504 | Acetylation | Uniprot | |
| T509 | O-Glycosylation | Uniprot | |
| T511 | Phosphorylation | Uniprot | |
| K512 | Acetylation | Uniprot | |
| T518 | Phosphorylation | Uniprot | |
| T525 | O-Glycosylation | Uniprot | |
| S529 | O-Glycosylation | Uniprot | |
| T532 | O-Glycosylation | Uniprot | |
| T540 | O-Glycosylation | Uniprot | |
| T541 | O-Glycosylation | Uniprot | |
| S544 | Phosphorylation | Uniprot | |
| S553 | O-Glycosylation | Uniprot | |
| T555 | O-Glycosylation | Uniprot | |
| T563 | O-Glycosylation | Uniprot | |
| T564 | O-Glycosylation | Uniprot | |
| T571 | O-Glycosylation | Uniprot | |
| T572 | O-Glycosylation | Uniprot | |
| T579 | O-Glycosylation | Uniprot | |
| T580 | O-Glycosylation | Uniprot | |
| T580 | Phosphorylation | Uniprot | |
| T587 | O-Glycosylation | Uniprot | |
| T588 | O-Glycosylation | Uniprot | |
| K590 | Acetylation | Uniprot | |
| T595 | O-Glycosylation | Uniprot | |
| T597 | Phosphorylation | Uniprot | |
| K598 | Acetylation | Uniprot | |
| T603 | O-Glycosylation | Uniprot | |
| T604 | O-Glycosylation | Uniprot | |
| T611 | O-Glycosylation | Uniprot | |
| T612 | O-Glycosylation | Uniprot | |
| T612 | Phosphorylation | Uniprot | |
| T616 | O-Glycosylation | Uniprot | |
| T619 | O-Glycosylation | Uniprot | |
| T625 | Phosphorylation | Uniprot | |
| T627 | O-Glycosylation | Uniprot | |
| T628 | Phosphorylation | Uniprot | |
| T651 | Phosphorylation | Uniprot | |
| T652 | Phosphorylation | Uniprot | |
| T660 | Phosphorylation | Uniprot | |
| T676 | O-Glycosylation | Uniprot | |
| T683 | O-Glycosylation | Uniprot | |
| T684 | O-Glycosylation | Uniprot | |
| T691 | O-Glycosylation | Uniprot | |
| T692 | O-Glycosylation | Uniprot | |
| T699 | O-Glycosylation | Uniprot | |
| T700 | O-Glycosylation | Uniprot | |
| T704 | O-Glycosylation | Uniprot | |
| T707 | O-Glycosylation | Uniprot | |
| T723 | O-Glycosylation | Uniprot | |
| T724 | O-Glycosylation | Uniprot | |
| T736 | O-Glycosylation | Uniprot | |
| T752 | Phosphorylation | Uniprot | |
| T753 | Phosphorylation | Uniprot | |
| T757 | Phosphorylation | Uniprot | |
| T760 | Phosphorylation | Uniprot | |
| T761 | Phosphorylation | Uniprot | |
| T768 | O-Glycosylation | Uniprot | |
| T769 | O-Glycosylation | Uniprot | |
| T776 | O-Glycosylation | Uniprot | |
| T777 | O-Glycosylation | Uniprot | |
| T792 | O-Glycosylation | Uniprot | |
| T793 | O-Glycosylation | Uniprot | |
| T805 | O-Glycosylation | Uniprot | |
| T806 | Phosphorylation | Uniprot | |
| S812 | O-Glycosylation | Uniprot | |
| T814 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| T829 | O-Glycosylation | Uniprot | |
| K832 | Ubiquitination | Uniprot | |
| T837 | O-Glycosylation | Uniprot | |
| T838 | O-Glycosylation | Uniprot | |
| K841 | Ubiquitination | Uniprot | |
| T846 | Phosphorylation | Uniprot | |
| T853 | Phosphorylation | Uniprot | |
| T859 | Phosphorylation | Uniprot | |
| T867 | Phosphorylation | Uniprot | |
| T868 | Phosphorylation | Uniprot | |
| S892 | O-Glycosylation | Uniprot | |
| T900 | O-Glycosylation | Uniprot | |
| T903 | Phosphorylation | Uniprot | |
| T930 | O-Glycosylation | Uniprot | |
| T931 | O-Glycosylation | Uniprot | |
| T932 | Phosphorylation | Uniprot | |
| T938 | Phosphorylation | Uniprot | |
| K939 | Acetylation | Uniprot | |
| T941 | Phosphorylation | Uniprot | |
| K947 | Acetylation | Uniprot | |
| T948 | Phosphorylation | Uniprot | |
| T954 | Phosphorylation | Uniprot | |
| T956 | Phosphorylation | Uniprot | |
| T958 | Phosphorylation | Uniprot | |
| S962 | O-Glycosylation | Uniprot | |
| S962 | Phosphorylation | Uniprot | |
| T963 | O-Glycosylation | Uniprot | |
| T968 | O-Glycosylation | Uniprot | |
| T975 | O-Glycosylation | Uniprot | |
| T978 | O-Glycosylation | Uniprot | |
| T978 | Phosphorylation | Uniprot | |
| T979 | O-Glycosylation | Uniprot | |
| T980 | O-Glycosylation | Uniprot | |
| T1039 | O-Glycosylation | Uniprot | |
| S1094 | Phosphorylation | Uniprot | |
| N1159 | N-Glycosylation | Uniprot | |
| T1161 | O-Glycosylation | Uniprot | |
| K1280 | Ubiquitination | Uniprot | |
| Y1365 | Phosphorylation | Uniprot | |
| S1397 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.
Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages.
PTMs:
N-glycosylated.
O-glycosylated; contains glycosaminoglycan chondroitin sulfate and keratan sulfate. O-glycosylated with sialylated oligosaccharides which are predominantly represented by the monosialylated core type I structure, NeuNAcalpha2-3Galbeta1-3GalNAc, with smaller amounts of disialylated O-glycans.
The disulfide bond between Cys-1146 and Cys-1403 is essential for protein cleavage.
Subcellular Location:
Secreted.
Tissue Specificity:
Highly expressed in synovial tissue, cartilage and liver and weakly in heart and lung. Isoform B is expressed in kidney, lung, liver, heart and brain. Isoform C and isoform D are widely expressed.
Subunit Structure:
Homodimer; disulfide-linked.
References
1). Punicalagin attenuates osteoarthritis progression via regulating Foxo1/Prg4/HIF3α axis. Bone, 2021
(PubMed: 34171516)
[IF=3.5]
2). Spatial distribution proteoglycans articular cartilage of femoral condyles and tibial plateau in experimental osteoarthritis in rats. Journal of Volgograd State Medical University, 2023
3). Пространственное распределение протеогликанов суставного хряща бедренных мыщелков и тибиального плато при экспериментальном остеоартрозе у крыс.
Научно-практический рецензируемый журнал
Клиническая и экспериментальная морфология
, 2024Restrictive clause
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