TET3 Antibody - #DF13335

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Product: TET3 Antibody
Catalog: DF13335
Description: Rabbit polyclonal antibody to TET3
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 179kDa; 194kD(Calculated).
Uniprot: O43151
RRID: AB_2846354

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TET3 Antibody detects endogenous levels of total TET3.
RRID:
AB_2846354
Cite Format: Affinity Biosciences Cat# DF13335, RRID:AB_2846354.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hCG_40738; KIAA0401; Methylcytosine dioxygenase TET3; Probable methylcytosine dioxygenase TET3; Tet methylcytosine dioxygenase 3; Tet oncogene family member 3; Tet3; TET3_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human TET3, corresponding to a region within the internal amino acids.

Uniprot:

O43151(TET3_HUMAN) >>Visit HPA database.

Gene(ID):
TET3(200424)
Expression:
O43151 TET3_HUMAN:

Expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes.

Sequence:
MSQFQVPLAVQPDLPGLYDFPQRQVMVGSFPGSGLSMAGSESQLRGGGDGRKKRKRCGTCEPCRRLENCGACTSCTNRRTHQICKLRKCEVLKKKVGLLKEVEIKAGEGAGPWGQGAAVKTGSELSPVDGPVPGQMDSGPVYHGDSRQLSASGVPVNGAREPAGPSLLGTGGPWRVDQKPDWEAAPGPAHTARLEDAHDLVAFSAVAEAVSSYGALSTRLYETFNREMSREAGNNSRGPRPGPEGCSAGSEDLDTLQTALALARHGMKPPNCNCDGPECPDYLEWLEGKIKSVVMEGGEERPRLPGPLPPGEAGLPAPSTRPLLSSEVPQISPQEGLPLSQSALSIAKEKNISLQTAIAIEALTQLSSALPQPSHSTPQASCPLPEALSPPAPFRSPQSYLRAPSWPVVPPEEHSSFAPDSSAFPPATPRTEFPEAWGTDTPPATPRSSWPMPRPSPDPMAELEQLLGSASDYIQSVFKRPEALPTKPKVKVEAPSSSPAPAPSPVLQREAPTPSSEPDTHQKAQTALQQHLHHKRSLFLEQVHDTSFPAPSEPSAPGWWPPPSSPVPRLPDRPPKEKKKKLPTPAGGPVGTEKAAPGIKPSVRKPIQIKKSRPREAQPLFPPVRQIVLEGLRSPASQEVQAHPPAPLPASQGSAVPLPPEPSLALFAPSPSRDSLLPPTQEMRSPSPMTALQPGSTGPLPPADDKLEELIRQFEAEFGDSFGLPGPPSVPIQDPENQQTCLPAPESPFATRSPKQIKIESSGAVTVLSTTCFHSEEGGQEATPTKAENPLTPTLSGFLESPLKYLDTPTKSLLDTPAKRAQAEFPTCDCVEQIVEKDEGPYYTHLGSGPTVASIRELMEERYGEKGKAIRIEKVIYTGKEGKSSRGCPIAKWVIRRHTLEEKLLCLVRHRAGHHCQNAVIVILILAWEGIPRSLGDTLYQELTDTLRKYGNPTSRRCGLNDDRTCACQGKDPNTCGASFSFGCSWSMYFNGCKYARSKTPRKFRLAGDNPKEEEVLRKSFQDLATEVAPLYKRLAPQAYQNQVTNEEIAIDCRLGLKEGRPFAGVTACMDFCAHAHKDQHNLYNGCTVVCTLTKEDNRCVGKIPEDEQLHVLPLYKMANTDEFGSEENQNAKVGSGAIQVLTAFPREVRRLPEPAKSCRQRQLEARKAAAEKKKIQKEKLSTPEKIKQEALELAGITSDPGLSLKGGLSQQGLKPSLKVEPQNHFSSFKYSGNAVVESYSVLGNCRPSDPYSMNSVYSYHSYYAQPSLTSVNGFHSKYALPSFSYYGFPSSNPVFPSQFLGPGAWGHSGSSGSFEKKPDLHALHNSLSPAYGGAEFAELPSQAVPTDAHHPTPHHQQPAYPGPKEYLLPKAPLLHSVSRDPSPFAQSSNCYNRSIKQEPVDPLTQAEPVPRDAGKMGKTPLSEVSQNGGPSHLWGQYSGGPSMSPKRTNGVGGSWGVFSSGESPAIVPDKLSSFGASCLAPSHFTDGQWGLFPGEGQQAASHSGGRLRGKPWSPCKFGNSTSALAGPSLTEKPWALGAGDFNSALKGSPGFQDKLWNPMKGEEGRIPAAGASQLDRAWQSFGLPLGSSEKLFGALKSEEKLWDPFSLEEGPAEEPPSKGAVKEEKGGGGAEEEEEELWSDSEHNFLDENIGGVAVAPAHGSILIECARRELHATTPLKKPNRCHPTRISLVFYQHKNLNQPNHGLALWEAKMKQLAERARARQEEAARLGLGQQEAKLYGKKRKWGGTVVAEPQQKEKKGVVPTRQALAVPTDSAVTVSSYAYTKVTGPYSRWI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity). Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG.

Subcellular Location:

Nucleus. Cytoplasm. Chromosome.
Note: At the zygotic stage, localizes in the male pronucleus, while it localizes to the cytoplasm at other preimplantation stages. Binds to the promoter of target genes, close to the transcription start site.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes.

Family&Domains:

The CXXC zinc finger mediates binding to CpG-DNA (PubMed:29276034). It mediates binding to DNA sequences containing unmethylated cytosine or 5-carboxylcytosine in 5'-CCG-3' DNA sequence motifs (By similarity).

Belongs to the TET family.

References

1). Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection. PLoS pathogens, 2022 (PubMed: 36070294) [IF=5.5]

Application: WB    Species: Human    Sample: A549 cells

Fig 7. The substrate binding domain of SPHK2 interacts with TET3 and HDAC1 and inhibits IFN-β transcription. (A) A549 cells transfected with pCMV-Myc or Myc-SPHK2 were immunoprecipitated using anti-Myc antibody, and then Myc-tagged SPHK2, HDAC1, and TET3 proteins were analyzed by immunoblotting. (B) A549 cells transfected with pRK11-Flag or Flag-TET3 were immunoprecipitated using anti-Flag antibody, and then SPHK2, HDAC1, and Flag-tagged TET3 proteins were analyzed by immunoblotting. (C) A549 cells transfected with pCMV-Myc or Myc-HDAC1 were immunoprecipitated using anti-Myc antibody, and then SPHK2, Myc-tagged HDAC1, and TET3 proteins were analyzed by immunoblotting. (D) A549 cells uninfected or infected with WSN were immunoprecipitated using anti-Human SPHK2 polyclonal antibody (Proteintech, 17096-1-AP), and then SPHK2, HDAC1 and TET3 proteins were analyzed by immunoblotting. (E) Diagram of SPHK2-FL (full length) and deletion of SPHK2 fragments (F1, F2 and F3). (F) A 549 cells transfected with pCMV-Myc, Myc-SPHK2-FL, Myc-SPHK2-F1, Myc-SPHK2-F2, and Myc-SPHK2-F3 were immunoprecipitated by using anti-Myc antibody, and then Myc-tagged SPHK2, Myc-tagged SPHK2-F3, HDAC1, and TET3 proteins were analyzed by immunoblotting. (G) HEK293 cells were transfected with pCMV-Myc, Myc-SPHK2-FL, Myc-SPHK2-F1, Myc-SPHK2-F2, or Myc-SPHK2-F3, and then infected with WSN virus at an MOI of 0.5. IFN-β activity was measured by using luciferase reporter assays. (H) A549 cells transfected with pCMV-Myc (Mock), Myc-SPHK2 or Myc-SPHK2-F3, and IFN-β promoter enrichment for Myc-tagged SPHK2 or Myc-tagged SPHK2-F3 was detected by ChIP-qPCR. (I) IFN-β promoter enrichment for Myc-tagged HDAC1 in sgCTRL A549 cells or sgSPHK2-1 A549 cells overexpressed with Myc-tagged HDAC1 was detected by ChIP-qPCR. (J) A549 cells were transfected with CTRL-siRNA or TET3-siRNA, and 24 h post-transfection, cells were overexpressed with SPHK2. IFN-β promoter enrichment for Myc-tagged SPHK2 was detected by ChIP-qPCR. (K) A549 cells were uninfected or infected with WSN, and IFN-β promoter enrichment for SPHK2 was determined by ChIP-qPCR. Data are representative of three independent experiments. ns, no significance; **, P

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