PICALM Antibody - #DF13343

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Product: PICALM Antibody
Catalog: DF13343
Description: Rabbit polyclonal antibody to PICALM
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 70kDa; 71kD(Calculated).
Uniprot: Q13492
RRID: AB_2846362

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PICALM Antibody detects endogenous levels of total PICALM.
RRID:
AB_2846362
Cite Format: Affinity Biosciences Cat# DF13343, RRID:AB_2846362.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CALM; Clathrin assembly lymphoid myeloid leukemia; Clathrin assembly lymphoid myeloid leukemia protein; CLTH; LAP; Phosphatidylinositol-binding clathrin assembly protein; PICAL_HUMAN; PICALM;

Immunogens

Immunogen:
Uniprot:

Q13492(PICAL_HUMAN) >>Visit HPA database.

Gene(ID):
PICALM(8301)
Expression:
Q13492 PICAL_HUMAN:

Expressed in all tissues examined.

Sequence:
MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPNDLLDLQQPTFHPSVHPMSTASQVASTWGDPFSATVDAVDDAIPSLNPFLTKSSGDVHLSISSDVSTFTTRTPTHEMFVGFTPSPVAQPHPSAGLNVDFESVFGNKSTNVIVDSGGFDELGGLLKPTVASQNQNLPVAKLPPSKLVSDDLDSSLANLVGNLGIGNGTTKNDVNWSQPGEKKLTGGSNWQPKVAPTTAWNAATMAPPVMAYPATTPTGMIGYGIPPQMGSVPVMTQPTLIYSQPVMRPPNPFGPVSGAQIQFM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13492 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S5 Phosphorylation
R9 Methylation
T11 Phosphorylation
S16 Phosphorylation
T18 Phosphorylation
S20 Phosphorylation
S23 Phosphorylation
K24 Ubiquitination
K28 Acetylation
T82 Phosphorylation
Y88 Phosphorylation
S107 Phosphorylation
K112 Ubiquitination
K134 Ubiquitination
Y138 Phosphorylation
T146 Phosphorylation
K163 Ubiquitination
K231 Ubiquitination
Y237 Phosphorylation
K238 Sumoylation
K252 Ubiquitination
K288 Ubiquitination
K291 Ubiquitination
S297 Phosphorylation
T301 Phosphorylation
S303 Phosphorylation
S307 Phosphorylation
S308 Phosphorylation
T312 Phosphorylation
S315 Phosphorylation
K318 Ubiquitination
K324 Ubiquitination
K336 Ubiquitination
T356 Phosphorylation
S359 Phosphorylation
T363 Phosphorylation
T379 Phosphorylation
S381 Phosphorylation
S450 Phosphorylation
S474 Phosphorylation
K515 Ubiquitination
S537 Phosphorylation
S543 Phosphorylation
K559 Ubiquitination
K570 Ubiquitination
K571 Ubiquitination
T573 Phosphorylation
R636 Methylation
S645 Phosphorylation

Research Backgrounds

Function:

Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).

Subcellular Location:

Cell membrane. Membrane>Clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle>Clathrin-coated vesicle. Nucleus.
Note: Colocalized with clathrin in the Golgi area (PubMed:10436022). Interaction with PIMREG may target PICALM to the nucleus in some cells (PubMed:16491119).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined.

Subunit Structure:

Binds to clathrin; involves primarily the C-terminal sequences, but the full-length protein is required for full binding capacity. Binds phosphatidylinositol 4,5- bisphosphate. Interacts with PIMREG; this interaction may change the subcellular location into the nucleus. Interacts with AP2A1 (via its alpha-appendage domain). Interacts (via N-terminus) with VAMP2; VAMP3; VAMP7 and VAMP8 (Via N-terminus). Interacts with LC3/MAP1LC3A.

Family&Domains:

Belongs to the PICALM/SNAP91 family.

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