KCNC3 Antibody - #DF13352
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13352, RRID:AB_2846371.
Fold/Unfold
Kcnc3; KCNC3_HUMAN; KSHIIID; KV3.3; Potassium voltage gated channel Shaw related subfamily member 3; Potassium voltage gated channel subfamily C member 3; Potassium voltage-gated channel subfamily C member 3; SCA13; Shaw related subfamily, member 3; Shaw related voltage gated potassium channel protein 3; Spinocerebellar ataxia 13; Voltage gated potassium channel protein KV3.3; Voltage gated potassium channel subunit Kv3.3; Voltage-gated potassium channel subunit Kv3.3;
Immunogens
- Q14003 KCNC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLSSVCVSSFRGRQGASKQQPAPPPQPPESPPPPPLPPQQQQPAQPGPAASPAGPPAPRGPGDRRAEPCPGLPAAAMGRHGGGGGDSGKIVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARFDYDPGADEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFEEELGFWGIDETDVEACCWMTYRQHRDAEEALDSFEAPDPAGAANAANAAGAHDGGLDDEAGAGGGGLDGAGGELKRLCFQDAGGGAGGPPGGAGGAGGTWWRRWQPRVWALFEDPYSSRAARYVAFASLFFILISITTFCLETHEGFIHISNKTVTQASPIPGAPPENITNVEVETEPFLTYVEGVCVVWFTFEFLMRITFCPDKVEFLKSSLNIIDCVAILPFYLEVGLSGLSSKAAKDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGADPDDILGSNHTYFKNIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPKKKNKHIPRPPQPGSPNYCKPDPPPPPPPHPHHGSGGISPPPPITPPSMGVTVAGAYPAGPHTHPGLLRGGAGGLGIMGLPPLPAPGEPCPLAQEEVIEINRADPRPNGDPAAAALAHEDCPAIDQPAMSPEDKSPITPGSRGRYSRDRACFLLTDYAPSPDGSIRKATGAPPLPPQDWRKPGPPSFLPDLNANAAAWISP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14003 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| Y102 | Phosphorylation | Uniprot | |
| C370 | S-Nitrosylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| T725 | Phosphorylation | Uniprot |
Research Backgrounds
Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient. The channel displays rapid activation and inactivation kinetics. It plays a role in the regulation of the frequency, shape and duration of action potentials in Purkinje cells. Required for normal survival of cerebellar neurons, probably via its role in regulating the duration and frequency of action potentials that in turn regulate the activity of voltage-gated Ca(2+) channels and cellular Ca(2+) homeostasis (By similarity). Required for normal motor function. Plays a role in the reorganization of the cortical actin cytoskeleton and the formation of actin veil structures in neuronal growth cones via its interaction with HAX1 and the Arp2/3 complex.
N-glycosylated.
Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Presynaptic cell membrane>Multi-pass membrane protein. Perikaryon. Cell projection>Axon. Cell projection>Dendrite. Cell projection>Dendritic spine membrane>Multi-pass membrane protein. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton.
Note: In Detected on Purkinje cell dendritic spines, positioned perisynaptically but also in extrasynaptic positions along the spine membranes (By similarity). Detected at presynaptic calices of Held (By similarity). Colocalizes with the cortical actin cytoskeleton and the Arp2/3 complex (PubMed:26997484).
Homotetramer. Heterotetramer with KCNC1. Interacts (via C-terminus) with HAX1. Identified in a complex with ACTR3, a subunit of the Arp2/3 complex; this interaction is indirect and depends on the presence of HAX1. Interaction with HAX1 modulates channel gating.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
The cytoplasmic N-terminus mediates N-type inactivation.
The C-terminal cytoplasmic tail contributes to the regulation of channel inactivation and to the interaction with HAX1 and the Arp2/3 complex.
Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.3/KCNC3 sub-subfamily.
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