Endomucin Antibody - #DF13357

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Product: Endomucin Antibody
Catalog: DF13357
Description: Rabbit polyclonal antibody to Endomucin
Application: WB IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 27kDa; 27kD(Calculated).
Uniprot: Q9ULC0
RRID: AB_2846376

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Endomucin Antibody detects endogenous levels of total Endomucin.
RRID:
AB_2846376
Cite Format: Affinity Biosciences Cat# DF13357, RRID:AB_2846376.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EMCN; EMCN2; Endomucin 2; Endomucin; Endomucin-2; Endothelial sialomucin; Gastric cancer antigen Ga34; MUC-14; MUC14; MUCEN_HUMAN; Mucin 14; Mucin-14;

Immunogens

Immunogen:

A synthesized peptide derived from human Endomucin, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9ULC0(MUCEN_HUMAN) >>Visit HPA database.

Gene(ID):
EMCN(51705)
Expression:
Q9ULC0 MUCEN_HUMAN:

Expressed in heart, kidney and lung.

Sequence:
MELLQVTILFLLPSICSSNSTGVLEAANNSLVVTTTKPSITTPNTESLQKNVVTPTTGTTPKGTITNELLKMSLMSTATFLTSKDEGLKATTTDVRKNDSIISNVTVTSVTLPNAVSTLQSSKPKTETQSSIKTTEIPGSVLQPDASPSKTGTLTSIPVTIPENTSQSQVIGTEGGKNASTSATSRSYSSIILPVVIALIVITLSVFVLVGLYRMCWKADPGTPENGNDQPQSDKESVKLLTVKTISHESGEHSAQGKTKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.

PTMs:

Highly O-glycosylated. Sialic acid-rich glycoprotein.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Membrane>Single-pass type I membrane protein.
Note: Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, kidney and lung.

References

1). Conformationally regulated "nanozyme-like" cerium oxide with multiple free radical scavenging activities for osteoimmunology modulation and vascularized osseointegration. Bioactive materials, 2023 (PubMed: 38186961) [IF=18.9]

Application: IF/ICC    Species: human    Sample: HUVECs

Fig. 4. Angiogenesis of HUVECs in pattern of DSS and CM, respectively. (A) Crystal violet staining images and (B) counting of the invasive HUVECs on Transwell membrane at 24 h. (C) Scratch images at 0 and 12 h and (D) quantified migration length of HUVECs. (E) Vessel tube formation images of HUVECs along with (F) total capillary length and (H) number of branch points per field at 12 h. (G)Fluorescence staining images and relative intensities of (I) CD31 (red) and (J) Emcn (green) in HUVECs at 5 d *p < 0.05.
2). Biomimetic and spatiotemporally sequential hydrogel delivery system with self-healing and adhesion: Triple growth factor for bone defect repair. Chemical Engineering Journal, 2023 [IF=13.3]
3). Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway. International journal of surgery (London, England), 2024 (PubMed: 39248296) [IF=12.5]

Application: IF/ICC    Species: Rat    Sample:

Figure 7. POL accelerated osteoporotic bone repair by stimulating osteogenesis and angiogenesis via up-regulating β-catenin expression. (A) Images of immunohistochemical staining for VEGFA (scale bar = 200 μm); (B) Quantification of immunohistochemical staining for VEGFA (n = 6); (C) Images of immunohistochemical staining for CD31 (scale bar = 200 μm); (D) Quantification of immunohistochemical staining for CD31 (n = 6); (E) Images of CD31 and EMCN immunofluorescence double-staining (scale bar = 100 μm); (F-H) Quantification of CD31 and EMCN immunofluorescence double-staining (n = 6); (I) Images of immunofluorescence staining for β-catenin (scale bar = 100 μm); (J) Quantification of immunofluorescence staining for β-catenin (n = 6). Data were presented as mean ± SEM. Compared with Sham group: * P < 0.05, **P < 0.01, ***P < 0.001. Compared with OVX + CON group: ### P < 0.001.
4). Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway. International journal of surgery (London, England), 2025 (PubMed: 39248296) [IF=12.5]
5). Chondroitin sulfate/silk fibroin hydrogel incorporating graphene oxide quantum dots with photothermal-effect promotes type H vessel-related wound healing. Carbohydrate polymers, 2024 (PubMed: 38553198) [IF=10.7]
6). Mesenchymal Stem Cell Aggregation-Released Extracellular Vesicles Induce CD31+ EMCN+ Vessels in Skin Regeneration and Improve Diabetic Wound Healing. Advanced healthcare materials, 2023 (PubMed: 36999744) [IF=10.0]
7). A vessel subtype beneficial for osteogenesis enhanced by strontium-doped sodium titanate nanorods by modulating macrophage polarization. Journal of Materials Chemistry B, 2020 (PubMed: 32627795) [IF=6.1]
8). Promoting diabetic oral mucosa wound healing with a light-responsive hydrogel adaptive to the microenvironment. Heliyon, 2024 (PubMed: 39435107) [IF=4.0]

Application: IF/ICC    Species: human    Sample:

Fig. 9 IF staining of diabetic oral mucosa defects across various treatment groups. Scale Bar: 200 μm.
9). Effect of M2-macrophage treated lymphatic endothelial cells on angiogenesis that promoted osteointegration. Experimental cell research, 2024 (PubMed: 38768700) [IF=3.3]
10). Soybean Isoflavones Alleviate Osteoarthritis Through Modulation of the TSC1/mTORC1 Signaling Pathway to Reduce Intrachondral Angiogenesis. Immunological investigations, 2024 (PubMed: 39360672) [IF=2.9]
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