SON Antibody - #DF13414

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Product: SON Antibody
Catalog: DF13414
Description: Rabbit polyclonal antibody to SON
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 263kDa; 264kD(Calculated).
Uniprot: P18583
RRID: AB_2846433

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SON Antibody detects endogenous levels of total SON.
RRID:
AB_2846433
Cite Format: Affinity Biosciences Cat# DF13414, RRID:AB_2846433.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BASS1; Bax antagonist selected in saccharomyces 1; DBP-5; Negative regulatory element-binding protein; NRE-binding protein; Protein DBP-5; Protein SON; SON; SON protein; SON_HUMAN; SON3;

Immunogens

Immunogen:
Uniprot:

P18583(SON_HUMAN) >>Visit HPA database.

Gene(ID):
SON(6651)
Expression:
P18583 SON_HUMAN:

Widely expressed, with the higher expression seen in leukocyte and heart.

Sequence:
MATNIEQIFRSFVVSKFREIQQELSSGRNEGQLNGETNTPIEGNQAGDAAASARSLPNEEIVQKIEEVLSGVLDTELRYKPDLKEGSRKSRCVSVQTDPTDEIPTKKSKKHKKHKNKKKKKKKEKEKKYKRQPEESESKTKSHDDGNIDLESDSFLKFDSEPSAVALELPTRAFGPSETNESPAVVLEPPVVSMEVSEPHILETLKPATKTAELSVVSTSVISEQSEQSVAVMPEPSMTKILDSFAAAPVPTTTLVLKSSEPVVTMSVEYQMKSVLKSVESTSPEPSKIMLVEPPVAKVLEPSETLVVSSETPTEVYPEPSTSTTMDFPESSAIEALRLPEQPVDVPSEIADSSMTRPQELPELPKTTALELQESSVASAMELPGPPATSMPELQGPPVTPVLELPGPSATPVPELPGPLSTPVPELPGPPATAVPELPGPSVTPVPQLSQELPGLPAPSMGLEPPQEVPEPPVMAQELPGLPLVTAAVELPEQPAVTVAMELTEQPVTTTELEQPVGMTTVEHPGHPEVTTATGLLGQPEATMVLELPGQPVATTALELPGQPSVTGVPELPGLPSATRALELSGQPVATGALELPGPLMAAGALEFSGQSGAAGALELLGQPLATGVLELPGQPGAPELPGQPVATVALEISVQSVVTTSELSTMTVSQSLEVPSTTALESYNTVAQELPTTLVGETSVTVGVDPLMAPESHILASNTMETHILASNTMDSQMLASNTMDSQMLASNTMDSQMLASSTMDSQMLATSSMDSQMLATSSMDSQMLATSTMDSQMLATSSMDSQMLATSSMDSQMLATSSMDSQMLATSSMDSQMLATSTMDSQMLATSTMDSQMLATSSMDSQMLASGTMDSQMLASGTMDAQMLASGTMDAQMLASSTQDSAMLGSKSPDPYRLAQDPYRLAQDPYRLGHDPYRLGHDAYRLGQDPYRLGHDPYRLTPDPYRMSPRPYRIAPRSYRIAPRPYRLAPRPLMLASRRSMMMSYAAERSMMSSYERSMMSYERSMMSPMAERSMMSAYERSMMSAYERSMMSPMAERSMMSAYERSMMSAYERSMMSPMADRSMMSMGADRSMMSSYSAADRSMMSSYSAADRSMMSSYTADRSMMSMAADSYTDSYTDTYTEAYMVPPLPPEEPPTMPPLPPEEPPMTPPLPPEEPPEGPALPTEQSALTAENTWPTEVPSSPSEESVSQPEPPVSQSEISEPSAVPTDYSVSASDPSVLVSEAAVTVPEPPPEPESSITLTPVESAVVAEEHEVVPERPVTCMVSETPAMSAEPTVLASEPPVMSETAETFDSMRASGHVASEVSTSLLVPAVTTPVLAESILEPPAMAAPESSAMAVLESSAVTVLESSTVTVLESSTVTVLEPSVVTVPEPPVVAEPDYVTIPVPVVSALEPSVPVLEPAVSVLQPSMIVSEPSVSVQESTVTVSEPAVTVSEQTQVIPTEVAIESTPMILESSIMSSHVMKGINLSSGDQNLAPEIGMQEIALHSGEEPHAEEHLKGDFYESEHGINIDLNINNHLIAKEMEHNTVCAAGTSPVGEIGEEKILPTSETKQRTVLDTYPGVSEADAGETLSSTGPFALEPDATGTSKGIEFTTASTLSLVNKYDVDLSLTTQDTEHDMVISTSPSGGSEADIEGPLPAKDIHLDLPSNNNLVSKDTEEPLPVKESDQTLAALLSPKESSGGEKEVPPPPKETLPDSGFSANIEDINEADLVRPLLPKDMERLTSLRAGIEGPLLASDVGRDRSAASPVVSSMPERASESSSEEKDDYEIFVKVKDTHEKSKKNKNRDKGEKEKKRDSSLRSRSKRSKSSEHKSRKRTSESRSRARKRSSKSKSHRSQTRSRSRSRRRRRSSRSRSKSRGRRSVSKEKRKRSPKHRSKSRERKRKRSSSRDNRKTVRARSRTPSRRSRSHTPSRRRRSRSVGRRRSFSISPSRRSRTPSRRSRTPSRRSRTPSRRSRTPSRRSRTPSRRSRTPSRRRRSRSVVRRRSFSISPVRLRRSRTPLRRRFSRSPIRRKRSRSSERGRSPKRLTDLDKAQLLEIAKANAAAMCAKAGVPLPPNLKPAPPPTIEEKVAKKSGGATIEELTEKCKQIAQSKEDDDVIVNKPHVSDEEEEEPPFYHHPFKLSEPKPIFFNLNIAAAKPTPPKSQVTLTKEFPVSSGSQHRKKEADSVYGEWVPVEKNGEENKDDDNVFSSNLPSEPVDISTAMSERALAQKRLSENAFDLEAMSMLNRAQERIDAWAQLNSIPGQFTGSTGVQVLTQEQLANTGAQAWIKKDQFLRAAPVTGGMGAVLMRKMGWREGEGLGKNKEGNKEPILVDFKTDRKGLVAVGERAQKRSGNFSAAMKDLSGKHPVSALMEICNKRRWQPPEFLLVHDSGPDHRKHFLFRVLRNGALTRPNCMFFLNRY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18583 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T3 Phosphorylation
R10 Methylation
K16 Acetylation
K16 Ubiquitination
T39 Phosphorylation
S55 Phosphorylation
K64 Ubiquitination
K80 Ubiquitination
K84 Ubiquitination
C92 S-Nitrosylation
S94 Phosphorylation
Y129 Phosphorylation
K141 Ubiquitination
S142 Phosphorylation
S152 Phosphorylation
S154 Phosphorylation
S160 Phosphorylation
S163 Phosphorylation
S182 Phosphorylation
T252 O-Glycosylation
T253 O-Glycosylation
K277 Ubiquitination
S278 Phosphorylation
S281 Phosphorylation
T282 Phosphorylation
S283 Phosphorylation
S287 Phosphorylation
K288 Acetylation
K288 Ubiquitination
S348 Phosphorylation
S354 Phosphorylation
T400 Phosphorylation
S910 Phosphorylation
Y921 Phosphorylation
R922 Methylation
Y928 Phosphorylation
R929 Methylation
Y935 Phosphorylation
R936 Methylation
Y942 Phosphorylation
R943 Methylation
Y949 Phosphorylation
R950 Methylation
Y956 Phosphorylation
R957 Methylation
T959 Phosphorylation
Y963 Phosphorylation
R964 Methylation
S966 Phosphorylation
R968 Methylation
Y970 Phosphorylation
R971 Methylation
R978 Methylation
R982 Methylation
R985 Methylation
R989 Methylation
R997 Methylation
S998 Phosphorylation
R1007 Methylation
S1012 Phosphorylation
R1015 Methylation
S1016 Phosphorylation
S1019 Phosphorylation
Y1020 Phosphorylation
R1022 Methylation
S1026 Phosphorylation
R1031 Methylation
S1035 Phosphorylation
Y1037 Phosphorylation
R1039 Methylation
S1043 Phosphorylation
Y1045 Phosphorylation
R1047 Methylation
S1051 Phosphorylation
R1056 Methylation
S1057 Phosphorylation
S1060 Phosphorylation
Y1062 Phosphorylation
R1064 Methylation
S1068 Phosphorylation
Y1070 Phosphorylation
R1072 Methylation
S1076 Phosphorylation
R1081 Methylation
S1082 Phosphorylation
S1085 Phosphorylation
R1090 Methylation
Y1096 Phosphorylation
S1097 Phosphorylation
R1101 Methylation
S1102 Phosphorylation
Y1107 Phosphorylation
S1108 Phosphorylation
R1112 Methylation
S1117 Phosphorylation
Y1118 Phosphorylation
S1216 Phosphorylation Q13535 (ATR)
T1262 Phosphorylation
T1336 Phosphorylation
S1480 Phosphorylation
S1509 Phosphorylation
T1555 Phosphorylation
S1556 Phosphorylation
S1570 Phosphorylation
T1572 Phosphorylation
K1573 Ubiquitination
T1576 Phosphorylation
T1645 Phosphorylation
S1646 Phosphorylation
S1648 Phosphorylation
S1651 Phosphorylation
S1670 Phosphorylation
K1677 Acetylation
T1679 Phosphorylation
K1686 Ubiquitination
S1688 Phosphorylation
S1697 Phosphorylation
S1701 Phosphorylation
S1702 Phosphorylation
T1746 Phosphorylation
S1747 Phosphorylation
S1759 Phosphorylation
S1766 Phosphorylation
S1769 Phosphorylation
S1773 Phosphorylation
S1774 Phosphorylation
S1780 Phosphorylation
S1782 Phosphorylation
S1783 Phosphorylation
S1784 Phosphorylation
Y1790 Phosphorylation
S1820 Phosphorylation
S1821 Phosphorylation
S1824 Phosphorylation
K1827 Acetylation
S1885 Phosphorylation
S1887 Phosphorylation
S1922 Phosphorylation
T1924 Phosphorylation
S1926 Phosphorylation
S1929 Phosphorylation
S1931 Phosphorylation
T1933 Phosphorylation
S1940 Phosphorylation
S1942 Phosphorylation
S1948 Phosphorylation
S1950 Phosphorylation
S1952 Phosphorylation
S1954 Phosphorylation
S1957 Phosphorylation
T1959 Phosphorylation
S1961 Phosphorylation
S1964 Phosphorylation
T1966 Phosphorylation
S1968 Phosphorylation
S1971 Phosphorylation
T1973 Phosphorylation
S1975 Phosphorylation
S1978 Phosphorylation
T1980 Phosphorylation
S1982 Phosphorylation
S1985 Phosphorylation
T1987 Phosphorylation
S1989 Phosphorylation
S1992 Phosphorylation
S1996 Phosphorylation
S2001 Phosphorylation
S2003 Phosphorylation
S2009 Phosphorylation
S2011 Phosphorylation
S2013 Phosphorylation
S2020 Phosphorylation
T2022 Phosphorylation
S2029 Phosphorylation
S2031 Phosphorylation
S2046 Phosphorylation
T2051 Phosphorylation
K2055 Acetylation
K2055 Ubiquitination
K2063 Acetylation
K2063 Ubiquitination
K2072 Ubiquitination
K2096 Ubiquitination
S2097 Phosphorylation
T2106 Phosphorylation
K2108 Ubiquitination
S2129 Phosphorylation
Y2139 Phosphorylation
K2149 Acetylation
K2161 Acetylation
T2163 Phosphorylation
T2172 Phosphorylation
K2173 Ubiquitination
S2179 Phosphorylation
S2181 Phosphorylation
K2186 Ubiquitination
S2190 Phosphorylation
Y2192 Phosphorylation
K2200 Ubiquitination
S2238 Phosphorylation
K2332 Ubiquitination
K2340 Ubiquitination
K2344 Ubiquitination
S2357 Phosphorylation
K2365 Ubiquitination
K2370 Ubiquitination
K2382 Ubiquitination
K2402 Ubiquitination

Research Backgrounds

Function:

RNA-binding protein that acts as a mRNA splicing cofactor by promoting efficient splicing of transcripts that possess weak splice sites. Specifically promotes splicing of many cell-cycle and DNA-repair transcripts that possess weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG. Probably acts by facilitating the interaction between Serine/arginine-rich proteins such as SRSF2 and the RNA polymerase II. Also binds to DNA; binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. May indirectly repress hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Essential for correct RNA splicing of multiple genes critical for brain development, neuronal migration and metabolism, including TUBG1, FLNA, PNKP, WDR62, PSMD3, PCK2, PFKL, IDH2, and ACY1.

Subcellular Location:

Nucleus speckle.
Note: Colocalizes with the pre-mRNA splicing factor SRSF2.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with the higher expression seen in leukocyte and heart.

Subunit Structure:

Interacts with SRSF2. Associates with the spliceosome. Interacts with the AML1-MTG8 (AML1-ETO) fusion protein, possibly leading to trigger signals inhibiting leukemogenesis.

Family&Domains:

Contains 8 types of repeats which are distributed in 3 regions.

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