MUM1 Antibody - #DF13418

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Product: MUM1 Antibody
Catalog: DF13418
Description: Rabbit polyclonal antibody to MUM1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 78kDa; 79kD(Calculated).
Uniprot: Q2TAK8
RRID: AB_2846437

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(88%), Sheep(100%)
Clonality:
Polyclonal
Specificity:
MUM1 Antibody detects endogenous levels of total MUM1.
RRID:
AB_2846437
Cite Format: Affinity Biosciences Cat# DF13418, RRID:AB_2846437.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EXPAND1; FLJ14868; FLJ22283; HSPC211; Melanoma associated antigen (mutated) 1; Melanoma ubiquitous mutated protein; MGC131891; MGC163315; MUM 1; MUM-1; MUM1; MUM1_HUMAN; Mutated melanoma associated antigen 1; Mutated melanoma-associated antigen 1; Protein expandere; PWWP domain containing protein MUM1; PWWP domain-containing protein MUM1;

Immunogens

Immunogen:
Uniprot:

Q2TAK8(PWP3A_HUMAN) >>Visit HPA database.

Gene(ID):
MUM1(84939)
Sequence:
MADAKYVLCRWEKRLWPAKVLARTATSTKNKRRKEYFLAVQILSLEEKIKVKSTEVEILEKSQIEAIASSLASQNEVPAAPLEELAYRRSLRVALDVLSEGSIWSQESSAGTGRADRSLRGKPMEHVSSPCDSNSSSLPRGDVLGSSRPHRRRPCVQQSLSSSFTCEKDPECKVDHKKGLRKSENPRGPLVLPAGGGAQDESGSRIHHKNWTLASKRGGNSAQKASLCLNGSSLSEDDTERDMGSKGGSWAAPSLPSGVREDDPCANAEGHDPGLPLGSLTAPPAPEPSACSEPGECPAKKRPRLDGSQRPPAVQLEPMAAGAAPSPGPGPGPRESVTPRSTARLGPPPSHASADATRCLPCPDSQKLEKECQSSEESMGSNSMRSILEEDEEDEEPPRVLLYHEPRSFEVGMLVWHKHKKYPFWPAVVKSVRQRDKKASVLYIEGHMNPKMKGFTVSLKSLKHFDCKEKQTLLNQAREDFNQDIGWCVSLITDYRVRLGCGSFAGSFLEYYAADISYPVRKSIQQDVLGTKLPQLSKGSPEEPVVGCPLGQRQPCRKMLPDRSRAARDRANQKLVEYIVKAKGAESHLRAILKSRKPSRWLQTFLSSSQYVTCVETYLEDEGQLDLVVKYLQGVYQEVGAKVLQRTNGDRIRFILDVLLPEAIICAISAVDEVDYKTAEEKYIKGPSLSYREKEIFDNQLLEERNRRRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Horse
88
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q2TAK8 As Substrate

Site PTM Type Enzyme
K5 Ubiquitination
Y6 Phosphorylation
K19 Ubiquitination
T28 Phosphorylation
K52 Ubiquitination
K122 Ubiquitination
S129 Phosphorylation
K209 Ubiquitination
S221 Phosphorylation
S226 Phosphorylation
S235 Phosphorylation
S326 Phosphorylation
S336 Phosphorylation
S365 Phosphorylation
S381 Phosphorylation
S383 Phosphorylation
K453 Ubiquitination
K470 Ubiquitination
K532 Ubiquitination
S537 Phosphorylation
K538 Ubiquitination
S540 Phosphorylation
S564 Phosphorylation
K581 Ubiquitination
K594 Acetylation
K597 Acetylation
Y631 Phosphorylation
Y636 Phosphorylation
K642 Ubiquitination
K685 Ubiquitination
K694 Ubiquitination

Research Backgrounds

Function:

Involved in the DNA damage response pathway by contributing to the maintenance of chromatin architecture. Recruited to the vicinity of DNA breaks by TP53BP1 and plays an accessory role to facilitate damage-induced chromatin changes and promoting chromatin relaxation. Required for efficient DNA repair and cell survival following DNA damage.

Subcellular Location:

Nucleus.
Note: Recruited to DNA damage sites via its interaction with the BRCT domain of TP53BP1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with TP53BP1 (via BRCT domain); the interaction is not dependent on its phosphorylation status. Binds nucleosomes. Interacts with trimethylated 'Lys-36' of histone H3 (H3K36me3) (in vitro).

Family&Domains:

The PWWP domain mediates the interaction with nucleosomes.

Belongs to the PWWP3A family.

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