FKBP25 Antibody - #DF13423

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Product: FKBP25 Antibody
Catalog: DF13423
Description: Rabbit polyclonal antibody to FKBP25
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 25kDa; 25kD(Calculated).
Uniprot: Q00688
RRID: AB_2846442

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FKBP25 Antibody detects endogenous levels of total FKBP25.
RRID:
AB_2846442
Cite Format: Affinity Biosciences Cat# DF13423, RRID:AB_2846442.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

25 kDa FK506-binding protein; 25 kDa FKBP; FK506 binding protein 25 T cell; FK506 binding protein 25, T cell, 25-KD; FK506 binding protein 3 (25kD); FK506-binding protein 3; FKBP 25; FKBP 3; FKBP-25; FKBP-3; FKBP25; FKBP3; FKBP3_HUMAN; Immunophilin FKBP25; Peptidyl-prolyl cis-trans isomerase FKBP3; PPIase; PPIase FKBP3; Rapamycin binding protein; Rapamycin-selective 25 kDa immunophilin; Rotamase;

Immunogens

Immunogen:
Uniprot:

Q00688(FKBP3_HUMAN) >>Visit HPA database.

Gene(ID):
FKBP3(2287)
Sequence:
MAAAVPQRAWTVEQLRSEQLPKKDIIKFLQEHGSDSFLAEHKLLGNIKNVAKTANKDHLVTAYNHLFETKRFKGTESISKVSEQVKNVKLNEDKPKETKSEETLDEGPPKYTKSVLKKGDKTNFPKKGDVVHCWYTGTLQDGTVFDTNIQTSAKKKKNAKPLSFKVGVGKVIRGWDEALLTMSKGEKARLEIEPEWAYGKKGQPDAKIPPNAKLTFEVELVDID

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q00688 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T11 Phosphorylation
R16 Methylation
K27 Ubiquitination
S34 Phosphorylation
S36 Phosphorylation
K42 Ubiquitination
K48 Ubiquitination
T75 Phosphorylation
S77 Phosphorylation
S79 Phosphorylation
K80 Ubiquitination
S82 Phosphorylation
K86 Ubiquitination
T98 Phosphorylation
S100 Phosphorylation
T147 Phosphorylation
S152 Phosphorylation
K154 Acetylation
K160 Acetylation
K160 Ubiquitination
S163 Phosphorylation
K165 Ubiquitination
K170 Acetylation
K170 Ubiquitination
R173 Methylation
T181 Phosphorylation
S183 Phosphorylation
K187 Methylation
Y198 Phosphorylation
K200 Acetylation
K200 Ubiquitination
K207 Ubiquitination

Research Backgrounds

Function:

FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the FKBP-type PPIase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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