iASPP Antibody - #DF13432

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Product: iASPP Antibody
Catalog: DF13432
Description: Rabbit polyclonal antibody to iASPP
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 89kDa; 89kD(Calculated).
Uniprot: Q8WUF5
RRID: AB_2846451

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
iASPP Antibody detects endogenous levels of total iASPP.
RRID:
AB_2846451
Cite Format: Affinity Biosciences Cat# DF13432, RRID:AB_2846451.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

IASPP; inhibitor of apoptosis stimulating protein of p53; Inhibitor of ASPP protein; NFkB interacting protein 1; NKIP1; PPP1R13B-like protein; PPP1R13L; Protein iASPP; protein phosphatase 1 regulatory (inhibitor) subunit 13 like; RAI; RelA-associated inhibitor;

Immunogens

Immunogen:
Uniprot:

Q8WUF5(IASPP_HUMAN) >>Visit HPA database.

Gene(ID):
PPP1R13L(10848)
Expression:
Q8WUF5 IASPP_HUMAN:

Highly expressed in heart, placenta and prostate. Weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte.

Sequence:
MDSEAFQSARDFLDMNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGPQAGPPSRPPRYSSSSIPEPFGSRGSPRKAATDGADTPFGRSESAPTLHPYSPLSPKGRPSSPRTPLYLQPDAYGSLDRATSPRPRAFDGAGSSLGRAPSPRPGPGPLRQQGPPTPFDFLGRAGSPRGSPLAEGPQAFFPERGPSPRPPATAYDAPASAFGSSLLGSGGSAFAPPLRAQDDLTLRRRPPKAWNESDLDVAYEKKPSQTASYERLDVFARPASPSLQLLPWRESSLDGLGGTGKDNLTSATLPRNYKVSPLASDRRSDAGSYRRSLGSAGPSGTLPRSWQPVSRIPMPPSSPQPRGAPRQRPIPLSMIFKLQNAFWEHGASRAMLPGSPLFTRAPPPKLQPQPQPQPQPQSQPQPQLPPQPQTQPQTPTPAPQHPQQTWPPVNEGPPKPPTELEPEPEIEGLLTPVLEAGDVDEGPVARPLSPTRLQPALPPEAQSVPELEEVARVLAEIPRPLKRRGSMEQAPAVALPPTHKKQYQQIISRLFHRHGGPGPGGPEPELSPITEGSEARAGPPAPAPPAPIPPPAPSQSSPPEQPQSMEMRSVLRKAGSPRKARRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIFATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
83
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WUF5 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S19 Phosphorylation
K28 Ubiquitination
T43 Phosphorylation
S48 Phosphorylation
S53 Phosphorylation
S65 Phosphorylation
Y70 Phosphorylation
S71 Phosphorylation
S74 Phosphorylation
S81 Phosphorylation
R82 Methylation
S84 Phosphorylation
K87 Methylation
K87 Ubiquitination
T90 Phosphorylation
T95 Phosphorylation
S100 Phosphorylation
S102 Phosphorylation
Y109 Phosphorylation
S110 Phosphorylation
S113 Phosphorylation
R117 Methylation
S119 Phosphorylation
S120 Phosphorylation
R122 Methylation
T123 Phosphorylation
Y126 Phosphorylation
Y132 Phosphorylation
S134 Phosphorylation
R137 Methylation
S140 Phosphorylation
R142 Methylation
R144 Methylation
S152 Phosphorylation
R155 Methylation
S158 Phosphorylation
R160 Methylation
R167 Methylation
T173 Phosphorylation
R180 Methylation
S183 Phosphorylation
R185 Methylation
S187 Phosphorylation
R200 Methylation
S203 Phosphorylation
R205 Methylation
T209 Phosphorylation
Y211 Phosphorylation
S216 Phosphorylation
S220 Phosphorylation
S225 Phosphorylation
R235 Methylation
T241 Phosphorylation
R243 Methylation
S253 Phosphorylation
Y259 Phosphorylation
S268 Phosphorylation
Y269 Phosphorylation
S280 Phosphorylation
S282 Phosphorylation
S291 Phosphorylation
S292 Phosphorylation
K301 Ubiquitination
T308 Phosphorylation
K314 Methylation
K314 Ubiquitination
S316 Phosphorylation
S320 Phosphorylation
S332 Phosphorylation
S335 Phosphorylation
S339 Phosphorylation
T341 Phosphorylation
R344 Methylation
S345 Phosphorylation
S357 Phosphorylation
S358 Phosphorylation
R362 Methylation
S395 Phosphorylation
T399 Phosphorylation
R400 Methylation
T491 Phosphorylation
K522 Methylation
K522 Ubiquitination
S526 Phosphorylation
K541 Ubiquitination
Y543 Phosphorylation
S567 Phosphorylation
S594 Phosphorylation
S597 Phosphorylation
S604 Phosphorylation
S616 Phosphorylation

Research Backgrounds

Function:

Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Predominantly cytoplasmic but also nuclear.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart, placenta and prostate. Weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte.

Subunit Structure:

Interacts with RELA NF-kappa-B subunit and with SP1 via its C-terminus part. Interacts (via SH3 domain and ANK repeats) with p53/TP53; the interaction inhibits pro-apoptotic activity of p53/TP53. Interacts with TP63 and TP73.

Family&Domains:

The N-terminal region is required for cytoplasmic localization.

The ANK repeats and the SH3 domain are required for specific interactions with p53/TP53.

Belongs to the iASPP family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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