LYRIC Antibody - #DF13437

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Product: LYRIC Antibody
Catalog: DF13437
Description: Rabbit polyclonal antibody to LYRIC
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 63kDa; 64kD(Calculated).
Uniprot: Q86UE4
RRID: AB_2846456

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(88%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
LYRIC Antibody detects endogenous levels of total LYRIC.
RRID:
AB_2846456
Cite Format: Affinity Biosciences Cat# DF13437, RRID:AB_2846456.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3D3; 3D3/LYRIC; AEG 1; AEG-1; AEG1; Astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC; LYRIC/3D3; LYRIC_HUMAN; Lysine rich CEACAM1 associated protein; Lysine rich CEACAM1 co isolated protein; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; MTDH; Protein LYRIC;

Immunogens

Immunogen:

A synthesized peptide derived from human LYRIC, corresponding to a region within C-terminal amino acids.

Uniprot:

Q86UE4(LYRIC_HUMAN) >>Visit HPA database.

Gene(ID):
MTDH(92140)
Expression:
Q86UE4 LYRIC_HUMAN:

Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.

Sequence:
MAARSWQDELAQQAEEGSARLREMLSVGLGFLRTELGLDLGLEPKRYPGWVILVGTGALGLLLLFLLGYGWAAACAGARKKRRSPPRKREEAAAVPAAAPDDLALLKNLRSEEQKKKNRKKLSEKPKPNGRTVEVAEGEAVRTPQSVTAKQPPEIDKKNEKSKKNKKKSKSDAKAVQNSSRHDGKEVDEGAWETKISHREKRQQRKRDKVLTDSGSLDSTIPGIENTITVTTEQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKTEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
88
Chicken
88
Rabbit
86
Xenopus
71
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Nucleus membrane>Single-pass membrane protein. Cell junction>Tight junction. Nucleus>Nucleolus. Cytoplasm>Perinuclear region.
Note: In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.

References

1). ELK3 Targeting AEG1 Promotes Migration and Invasion of Ovarian Cancer Cells under Hypoxia. Biological & pharmaceutical bulletin, 2023 (PubMed: 37394639) [IF=1.7]

Application: WB    Species: human    Sample: OC tissue

Fig. 1. Characterization of ELK3 in Ovarian Cancer (OC) Cells under Hypoxia and ELK3 and AEG1 Were Highly Expressed in Human OC Tissues Data from the GEO database (https://www.ncbi.nlm.nih.gov/gds), and the ID number is GSE53012. The sequencing samples included 3 SKOV3 cell samples (GSM1280344–GSM1280346) and 6 SKOV3 cell samples under hypoxia. (GSM1280338–GSM1280343). (a) Heat map showing differentially expressed genes and the expression levels of ELK family members. (b) Volcano map showing significantly differentially expressed genes. Red, up-regulation; Green: down-upregulation. (c) Correlation analysis between ELK3 and AEG1 from GEPIA website (http://gepia.cancer-pku.cn/). (d) Circos showing differentially expressed genes at chromosomal locations. (e) The signaling pathway was performed and visualized by KEGG. (f) The relative mRNA expression of ELK3 and AEG1 in 10 cases of OC tissues and 8 cases of paracancerous tissues, and the correlation analysis. (g) Representative protein bands for ELK3 and AEG1 from 4 cases of OC tissue and 4 cases of paracancerous tissue were visualized in the Western blot assay, and the correlation analysis. Hyp, hypoxia; N, paracancerous tissue; T, ovarian cancer tissue. Data represented the mean ± standard deviation. + p

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