Slap Antibody - #DF13444

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Product: Slap Antibody
Catalog: DF13444
Description: Rabbit polyclonal antibody to Slap
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Dog
Mol.Wt.: 31kDa; 31kD(Calculated).
Uniprot: Q13239
RRID: AB_2846463

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Slap Antibody detects endogenous levels of total Slap.
RRID:
AB_2846463
Cite Format: Affinity Biosciences Cat# DF13444, RRID:AB_2846463.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hSLAP; SLA 1; sla; SLA1; SLAP 1; SLAP; SLAP-1; SLAP1; SLAP1_HUMAN; Src like adapter; Src like adapter protein 1; Src like adaptor; SRC-like-adapter; Src-like-adapter protein 1;

Immunogens

Immunogen:
Uniprot:

Q13239(SLAP1_HUMAN) >>Visit HPA database.

Gene(ID):
SLA(6503)
Expression:
Q13239 SLAP1_HUMAN:

Expressed in lung and fetal brain. Weakly expressed in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas.

Sequence:
MGNSMKSTPAPAERPLPNPEGLDSDFLAVLSDYPSPDISPPIFRRGEKLRVISDEGGWWKAISLSTGRESYIPGICVARVYHGWLFEGLGRDKAEELLQLPDTKVGSFMIRESETKKGFYSLSVRHRQVKHYRIFRLPNNWYYISPRLTFQCLEDLVNHYSEVADGLCCVLTTPCLTQSTAAPAVRASSSPVTLRQKTVDWRRVSRLQEDPEGTENPLGVDESLFSYGLRESIASYLSLTSEDNTSFDRKKKSISLMYGGSKRKSSFFSSPPYFED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13239 As Substrate

Site PTM Type Enzyme
K60 Ubiquitination
K93 Ubiquitination
K104 Ubiquitination
K117 Ubiquitination
Y120 Phosphorylation
S189 Phosphorylation
S190 Phosphorylation
T193 Phosphorylation
K197 Ubiquitination
S205 Phosphorylation
S235 Phosphorylation
S238 Phosphorylation
T240 Phosphorylation
K252 Acetylation
K252 Ubiquitination
S253 Phosphorylation
S255 Phosphorylation
Y258 Phosphorylation
S261 Phosphorylation
K262 Acetylation
K262 Ubiquitination
K264 Ubiquitination
S266 Phosphorylation
Y273 Phosphorylation

Research Backgrounds

Function:

Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.

Subcellular Location:

Cytoplasm. Endosome.
Note: Colocalizes with endosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in lung and fetal brain. Weakly expressed in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas.

Subunit Structure:

Interacts with EPHA2, VAV1, LCP2 and PDGFRB (By similarity). Homodimer. Homodimerization and interaction with phosphorylated CBL occurs via its C-terminal domain. Interacts with phosphorylated proteins ZAP70, CD3Z, SYK and LAT via its SH2 domain.

Family&Domains:

The C-terminal domain is essential for the homodimerization and the interaction with CBL. While the interaction with CBL is apparently mediated via the hydrophobic region of this domain, the highly charged region is apparently required for the homodimerization.

Restrictive clause

 

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