SAFB Antibody - #DF13446

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Product: SAFB Antibody
Catalog: DF13446
Description: Rabbit polyclonal antibody to SAFB
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 102kDa; 103kD(Calculated).
Uniprot: Q15424
RRID: AB_2846465

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
SAFB Antibody detects endogenous levels of total SAFB.
RRID:
AB_2846465
Cite Format: Affinity Biosciences Cat# DF13446, RRID:AB_2846465.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp779C1727; glutathione S transferase fusion protein; HAP; HET; HSP27 ERE TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAF B; SAF-B; SAF-B1; SAFB 1; SAFB; SAFB1; SAFB1_HUMAN; Scaffold attachment factor B1;

Immunogens

Immunogen:
Uniprot:

Q15424(SAFB1_HUMAN) >>Visit HPA database.

Gene(ID):
SAFB(6294)
Expression:
Q15424 SAFB1_HUMAN:

Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.

Sequence:
MAETLSGLGDSGAAGAAALSSASSETGTRRLSDLRVIDLRAELRKRNVDSSGNKSVLMERLKKAIEDEGGNPDEIEITSEGNKKTSKRSSKGRKPEEEGVEDNGLEENSGDGQEDVETSLENLQDIDIMDISVLDEAEIDNGSVADCVEDDDADNLQESLSDSRELVEGEMKELPEQLQEHAIEDKETINNLDTSSSDFTILQEIEEPSLEPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASESTAHAQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSPEARDSKEDGRKFDFDACNEVPPAPKESSTSEGADQKMSSPEDDSDTKRLSKEEKGRSSCGRNFWVSGLSSTTRATDLKNLFSKYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCINHLHKTELHGKMISVEKAKNEPVGKKTSDKRDSDGKKEKSSNSDRSTNLKRDDKCDRKDDAKKGDDGSGEKSKDQDDQKPGPSERSRATKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKEPRKSRDSESHSRVRERSEREQRMQAQWEREERERLEIARERLAFQRQRLERERMERERLERERMHVEHERRREQERIHREREELRRQQELRYEQERRPAVRRPYDLDRRDDAYWPEAKRAALDERYHSDFNRQDRFHDFDHRDRGRYPDHSVDRREGSRSMMGEREGQHYPERHGGPERHGRDSRDGWGGYGSDKRMSEGRGLPPPPRRDWGDHGRREDDRSWQGTADGGMMDRDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHPIPHGGMQGGFGGQSRGSRPSDARFTRRY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Chicken
88
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15424 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T4 Phosphorylation
S6 Phosphorylation
S11 Phosphorylation
S20 Phosphorylation
S21 Phosphorylation
S23 Phosphorylation
S24 Phosphorylation
T26 Phosphorylation
R29 Methylation
S32 Phosphorylation
R35 Methylation
S55 Phosphorylation
R60 Methylation
S79 Phosphorylation
K83 Acetylation
K83 Ubiquitination
K84 Acetylation
S143 Phosphorylation
K172 Sumoylation
T188 Phosphorylation
T194 Phosphorylation
S195 Phosphorylation
S196 Phosphorylation
S197 Phosphorylation
T200 Phosphorylation
S209 Phosphorylation
K226 Methylation
K226 Sumoylation
K226 Ubiquitination
S227 Phosphorylation
K231 Sumoylation
K231 Ubiquitination
S234 Phosphorylation
S235 Phosphorylation
T245 Phosphorylation
S246 Phosphorylation
S247 Phosphorylation
K253 Sumoylation
S263 Phosphorylation
S275 Phosphorylation
K285 Ubiquitination
S288 Phosphorylation
K294 Acetylation
K294 Sumoylation
K294 Ubiquitination
S321 Phosphorylation
S322 Phosphorylation
S331 Phosphorylation
S332 Phosphorylation
T340 Phosphorylation
S344 Phosphorylation
S350 Phosphorylation
K356 Acetylation
K356 Ubiquitination
K370 Ubiquitination
S373 Phosphorylation
T374 Phosphorylation
S375 Phosphorylation
K381 Sumoylation
S383 Phosphorylation
S384 Phosphorylation
S389 Phosphorylation
K392 Ubiquitination
S395 Phosphorylation
S414 Phosphorylation
S415 Phosphorylation
T416 Phosphorylation
K423 Acetylation
K423 Ubiquitination
K428 Acetylation
K428 Ubiquitination
K431 Ubiquitination
K436 Acetylation
K436 Ubiquitination
T439 Phosphorylation
S443 Phosphorylation
T453 Phosphorylation
K469 Ubiquitination
K475 Acetylation
K475 Ubiquitination
S492 Phosphorylation
K500 Acetylation
K501 Acetylation
S504 Phosphorylation
S507 Phosphorylation
R509 Methylation
K543 Sumoylation
S555 Phosphorylation
S557 Phosphorylation
T560 Phosphorylation
T563 Phosphorylation
K568 Acetylation
S569 Phosphorylation
S576 Phosphorylation
K578 Sumoylation
T579 Phosphorylation
S580 Phosphorylation
S582 Phosphorylation
S587 Phosphorylation
S589 Phosphorylation
S601 Phosphorylation
S604 Phosphorylation
K607 Acetylation
K607 Ubiquitination
K609 Acetylation
S614 Phosphorylation
S617 Phosphorylation
S621 Phosphorylation
S627 Phosphorylation
R712 Methylation
Y723 Phosphorylation
K728 Methylation
S738 Phosphorylation
R754 Methylation
S761 Phosphorylation
R764 Methylation
S770 Phosphorylation
R783 Methylation
S794 Phosphorylation
Y801 Phosphorylation
S803 Phosphorylation
K805 Acetylation
K805 Methylation
S808 Phosphorylation
R811 Methylation
R818 Methylation
S832 Phosphorylation
S855 Phosphorylation
R868 Methylation
R874 Methylation
R884 Methylation
R902 Methylation
S904 Phosphorylation
R905 Methylation
S907 Phosphorylation
R910 Methylation

Research Backgrounds

Function:

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity). Can inhibit cell proliferation.

PTMs:

Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.

Subunit Structure:

Monomer. Can form homodimers. Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and TRA2B/SFRS10 (By similarity). Interacts with isoform 1 and isoform 2 of SRPK1 and inhibits its activity. Interacts with RBMX (By similarity). Interacts with FUS.

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