SCC112 Antibody - #DF13456

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Product: SCC112 Antibody
Catalog: DF13456
Description: Rabbit polyclonal antibody to SCC112
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 150kDa; 151kD(Calculated).
Uniprot: Q29RF7
RRID: AB_2846475

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SCC112 Antibody detects endogenous levels of total SCC112.
RRID:
AB_2846475
Cite Format: Affinity Biosciences Cat# DF13456, RRID:AB_2846475.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cell proliferation inducing protein 54; Cell proliferation-inducing gene 54 protein; KIAA0648; PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae); PDS5A; PDS5A_HUMAN; PIG54; SCC-112; SCC112; Sister chromatid cohesion protein 112; Sister chromatid cohesion protein PDS5 homolog A;

Immunogens

Immunogen:
Uniprot:

Q29RF7(PDS5A_HUMAN) >>Visit HPA database.

Gene(ID):
PDS5A(23244)
Expression:
Q29RF7 PDS5A_HUMAN:

Highest level in colon. Low levels in lung, ovary, breast and kidney. Reduced level in renal tumor tissue. Isoform 2 is expressed in kidney.

Sequence:
MDFTAQPKPATALCGVVSADGKIAYPPGVKEITDKITTDEMIKRLKMVVKTFMDMDQDSEDEKQQYLPLALHLASEFFLRNPNKDVRLLVACCLADIFRIYAPEAPYTSHDKLKDIFLFITRQLKGLEDTKSPQFNRYFYLLENLAWVKSYNICFELEDCNEIFIQLFRTLFSVINNSHNKKVQMHMLDLMSSIIMEGDGVTQELLDSILINLIPAHKNLNKQSFDLAKVLLKRTVQTIEACIANFFNQVLVLGRSSVSDLSEHVFDLIQELFAIDPHLLLSVMPQLEFKLKSNDGEERLAVVRLLAKLFGSKDSDLATQNRPLWQCFLGRFNDIHVPVRLESVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVTIITAAKRDLALVNDQLLGFVRERTLDKRWRVRKEAMMGLAQLYKKYCLHGEAGKEAAEKVSWIKDKLLHIYYQNSIDDKLLVEKIFAQYLVPHNLETEERMKCLYYLYASLDPNAVKALNEMWKCQNMLRSHVRELLDLHKQPTSEANCSAMFGKLMTIAKNLPDPGKAQDFVKKFNQVLGDDEKLRSQLELLISPTCSCKQADICVREIARKLANPKQPTNPFLEMVKFLLERIAPVHIDSEAISALVKLMNKSIEGTADDEEEGVSPDTAIRSGLELLKVLSFTHPTSFHSAETYESLLQCLRMEDDKVAEAAIQIFRNTGHKIETDLPQIRSTLIPILHQKAKRGTPHQAKQAVHCIHAIFTNKEVQLAQIFEPLSRSLNADVPEQLITPLVSLGHISMLAPDQFASPMKSVVANFIVKDLLMNDRSTGEKNGKLWSPDEEVSPEVLAKVQAIKLLVRWLLGMKNNQSKSANSTLRLLSAMLVSEGDLTEQKRISKSDMSRLRLAAGSAIMKLAQEPCYHEIITPEQFQLCALVINDECYQVRQIFAQKLHKALVKLLLPLEYMAIFALCAKDPVKERRAHARQCLLKNISIRREYIKQNPMATEKLLSLLPEYVVPYMIHLLAHDPDFTRSQDVDQLRDIKECLWFMLEVLMTKNENNSHAFMKKMAENIKLTRDAQSPDESKTNEKLYTVCDVALCVINSKSALCNADSPKDPVLPMKFFTQPEKDFCNDKSYISEETRVLLLTGKPKPAGVLGAVNKPLSATGRKPYVRSTGTETGSNINVNSELNPSTGNRSREQSSEAAETGVSENEENPVRIISVTPVKNIDPVKNKEINSDQATQGNISSDRGKKRTVTAAGAENIQQKTDEKVDESGPPAPSKPRRGRRPKSESQGNATKNDDLNKPINKGRKRAAVGQESPGGLEAGNAKAPKLQDLAKKAAPAERQIDLQR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Zebrafish
86
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q29RF7 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K30 Ubiquitination
K35 Ubiquitination
T38 Phosphorylation
K43 Ubiquitination
K50 Ubiquitination
T51 Phosphorylation
S59 Phosphorylation
Y101 Phosphorylation
Y107 Phosphorylation
T108 Phosphorylation
S109 Phosphorylation
K131 Ubiquitination
S192 Phosphorylation
S193 Phosphorylation
K222 Ubiquitination
K292 Ubiquitination
K308 Ubiquitination
K359 Ubiquitination
Y364 Phosphorylation
K366 Acetylation
K366 Ubiquitination
K389 Acetylation
Y426 Phosphorylation
K427 Acetylation
K428 Ubiquitination
K442 Ubiquitination
K507 Ubiquitination
K524 Ubiquitination
K544 Ubiquitination
K557 Acetylation
K558 Ubiquitination
K568 Ubiquitination
K601 Ubiquitination
K633 Ubiquitination
K637 Ubiquitination
K708 Ubiquitination
K727 Ubiquitination
S762 Phosphorylation
S793 Phosphorylation
K805 Ubiquitination
R812 Methylation
K820 Acetylation
K820 Ubiquitination
S823 Phosphorylation
K835 Ubiquitination
K855 Ubiquitination
S865 Phosphorylation
S870 Phosphorylation
T875 Phosphorylation
K882 Acetylation
K882 Ubiquitination
S883 Phosphorylation
S894 Phosphorylation
K938 Ubiquitination
Y949 Phosphorylation
K974 Acetylation
K974 Ubiquitination
S977 Phosphorylation
Y982 Phosphorylation
K984 Ubiquitination
K1058 Ubiquitination
S1065 Phosphorylation
C1079 S-Nitrosylation
C1084 S-Nitrosylation
S1097 Phosphorylation
K1106 Ubiquitination
K1113 Acetylation
K1113 Ubiquitination
K1119 Ubiquitination
K1134 Ubiquitination
K1136 Ubiquitination
K1146 Acetylation
K1146 Ubiquitination
S1149 Phosphorylation
T1151 Phosphorylation
T1162 Phosphorylation
S1172 Phosphorylation
S1177 Phosphorylation
T1178 Phosphorylation
S1182 Phosphorylation
S1186 Phosphorylation
S1187 Phosphorylation
T1192 Phosphorylation
S1195 Phosphorylation
S1206 Phosphorylation
T1208 Phosphorylation
K1211 Acetylation
K1211 Ubiquitination
K1219 Acetylation
K1219 Ubiquitination
S1223 Phosphorylation
T1227 Phosphorylation
S1232 Phosphorylation
S1233 Phosphorylation
R1235 Methylation
T1240 Phosphorylation
K1267 Acetylation
S1276 Phosphorylation
S1278 Phosphorylation
K1290 Acetylation
K1294 Acetylation
S1305 Phosphorylation
K1315 Ubiquitination

Research Backgrounds

Function:

Probable regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.

Subcellular Location:

Nucleus.
Note: Associated with chromatin through most of the cell cycle. Dissociates from chromatin in late prophase, reassociates during late telophase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest level in colon. Low levels in lung, ovary, breast and kidney. Reduced level in renal tumor tissue. Isoform 2 is expressed in kidney.

Subunit Structure:

Interacts with the cohesin complex. Interacts with WAPL (via FGF motifs) or CDCA5 (via the FGF motif); the interaction is direct, cohesin-dependent and competitive. Interacts with SMC3. Interacts with TP63.

Family&Domains:

Belongs to the PDS5 family.

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