HLTF Antibody - #DF13471

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Product: HLTF Antibody
Catalog: DF13471
Description: Rabbit polyclonal antibody to HLTF
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 113kDa; 114kD(Calculated).
Uniprot: Q14527
RRID: AB_2846490

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Zebrafish(83%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
HLTF Antibody detects endogenous levels of total HLTF.
RRID:
AB_2846490
Cite Format: Affinity Biosciences Cat# DF13471, RRID:AB_2846490.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA-binding protein/plasminogen activator inhibitor 1 regulator; Helicase like transcription factor; Helicase-like transcription factor; HIP116; HIP116A; HLTF 1; Hltf; HLTF_HUMAN; HLTF1; p113; RING finger protein 80; RNF80; SMARC A3; SMARCA 3; SMARCA3; SNF2-like 3; SNF2L3; Sucrose nonfermenting protein 2 like 3; Sucrose nonfermenting protein 2-like 3; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 3; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a3; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; ZBU 1; ZBU1;

Immunogens

Immunogen:
Uniprot:

Q14527(HLTF_HUMAN) >>Visit HPA database.

Gene(ID):
HLTF(6596)
Expression:
Q14527 HLTF_HUMAN:

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.

Sequence:
MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSLRGHVVGLRYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANNAFTMPLHMTFWGKEENRKAVSDQLKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKADGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKGKLKNVQSETKGRAKAGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWHRTIQRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICLDSLTVPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECPPEELARDSEKKSDMEWTSSSKINALMHALTDLRKKNPNIKSLVVSQFTTFLSLIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNKKRELAAGAFGTKKPNADEMKQAKINEIRTLIDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
88
Horse
88
Sheep
88
Xenopus
88
Rabbit
88
Zebrafish
83
Bovine
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14527 As Substrate

Site PTM Type Enzyme
K6 Ubiquitination
K12 Ubiquitination
R27 Methylation
S29 Phosphorylation
Y93 Phosphorylation
K95 Ubiquitination
K99 Ubiquitination
K112 Ubiquitination
K113 Ubiquitination
Y121 Phosphorylation
K158 Acetylation
K158 Ubiquitination
K165 Acetylation
K165 Ubiquitination
K170 Ubiquitination
K176 Ubiquitination
Y195 Phosphorylation
K216 Ubiquitination
K222 Ubiquitination
T238 Phosphorylation
K245 Ubiquitination
K258 Ubiquitination
K281 Acetylation
K281 Ubiquitination
K329 Acetylation
K330 Acetylation
K330 Ubiquitination
K340 Ubiquitination
K349 Ubiquitination
S354 Phosphorylation
K355 Ubiquitination
K370 Ubiquitination
S373 Phosphorylation
S378 Phosphorylation
S381 Phosphorylation
T390 Phosphorylation
Y394 Phosphorylation
S397 Phosphorylation
S398 Phosphorylation
S400 Phosphorylation
T405 Phosphorylation
K417 Sumoylation
S421 Phosphorylation
K424 Ubiquitination
K433 Ubiquitination
K452 Ubiquitination
K458 Ubiquitination
T469 Phosphorylation
K523 Ubiquitination
K541 Ubiquitination
S544 Phosphorylation
S548 Phosphorylation
K571 Ubiquitination
K606 Ubiquitination
S635 Phosphorylation
K638 Ubiquitination
T641 Phosphorylation
T645 Phosphorylation
K653 Ubiquitination
K662 Ubiquitination
K676 Ubiquitination
K682 Ubiquitination
T736 Phosphorylation
K824 Ubiquitination
S858 Phosphorylation
S884 Phosphorylation
K952 Ubiquitination
K959 Ubiquitination
K963 Ubiquitination
K972 Ubiquitination
K996 Ubiquitination
K999 Ubiquitination

Research Backgrounds

Function:

Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.

Subcellular Location:

Cytoplasm. Nucleus. Nucleus>Nucleolus. Nucleus>Nucleoplasm.
Note: Nuclear localization is stimulated by progesterone.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.

Subunit Structure:

Interacts with SP1 and SP3 independently of DNA; the interaction with these transcriptional factors may be required for basal transcription of target genes. Interacts with EGR1; the interaction requires prior binding to DNA and represses c-Rel via a DNA looping mechanism (By similarity). Interacts with GATA4 (By similarity). Interacts with PCNA; the interaction promotes polyubiquitination of PCNA through association with the UBE2B-RAD18 and UBE2V2-UBE2N ubiquitin ligase complexes. Interacts with RAD18, SHPRH, UBE2V2 and UBE2N.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.

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