HIRA Antibody - #DF13473
| Product: | HIRA Antibody |
| Catalog: | DF13473 |
| Description: | Rabbit polyclonal antibody to HIRA |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 111kDa; 112kD(Calculated). |
| Uniprot: | P54198 |
| RRID: | AB_2846492 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13473, RRID:AB_2846492.
Fold/Unfold
DGCR1; DiGeorge critical region gene 1; HIR; HIR histone cell cycle regulation defective homolog A; HIRA; HIRA protein; HIRA_HUMAN; Histone cell cycle regulator; Protein HIRA; TUP 1; TUP1 like enhancer of split protein 1; TUP1-like enhancer of split protein 1; TUPLE1;
Immunogens
Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
- P54198 HIRA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQDSGKVVIWNMSPVLQEDDEKDENIPKMLCQMDNHLACVNCVRWSNSGMYLASGGDDKLIMVWKRATYIGPSTVFGSSGKLANVEQWRCVSILRNHSGDVMDVAWSPHDAWLASCSVDNTVVIWNAVKFPEILATLRGHSGLVKGLTWDPVGKYIASQADDRSLKVWRTLDWQLETSITKPFDECGGTTHVLRLSWSPDGHYLVSAHAMNNSGPTAQIIEREGWKTNMDFVGHRKAVTVVKFNPKIFKKKQKNGSSAKPSCPYCCCAVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDGSVAFLDFSQDELGDPLSEEEKSRIHQSTYGKSLAIMTEAQLSTAVIENPEMLKYQRRQQQQQLDQKSAATREMGSATSVAGVVNGESLEDIRKNLLKKQVETRTADGRRRITPLCIAQLDTGDFSTAFFNSIPLSGSLAGTMLSSHSSPQLLPLDSSTPNSFGASKPCTEPVVAASARPAGDSVNKDSMNATSTPAALSPSVLTTPSKIEPMKAFDSRFTERSKATPGAPALTSMTPTAVERLKEQNLVKELRPRDLLESSSDSDEKVPLAKASSLSKRKLELEVETVEKKKKGRPRKDSRLMPVSLSVQSPAALTAEKEAMCLSAPALALKLPIPSPQRAFTLQVSSDPSMYIEVENEVTVVGGVKLSRLKCNREGKEWETVLTSRILTAAGSCDVVCVACEKRMLSVFSTCGRRLLSPILLPSPISTLHCTGSYVMALTAAATLSVWDVHRQVVVVKEESLHSILAGSDMTVSQILLTQHGIPVMNLSDGKAYCFNPSLSTWNLVSDKQDSLAQCADFRSSLPSQDAMLCSGPLAIIQGRTSNSGRQAARLFSVPHVVQQETTLAYLENQVAAALTLQSSHEYRHWLLVYARYLVNEGFEYRLREICKDLLGPVHYSTGSQWESTVVGLRKRELLKELLPVIGQNLRFQRLFTECQEQLDILRDK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P54198 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K27 | Ubiquitination | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| Y404 | Phosphorylation | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| T544 | Phosphorylation | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| T554 | Phosphorylation | Uniprot | |
| T555 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S557 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| S573 | Phosphorylation | Uniprot | |
| T576 | Phosphorylation | Uniprot | |
| T583 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S584 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T586 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T588 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K594 | Ubiquitination | Uniprot | |
| K600 | Acetylation | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| S612 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| T637 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S656 | Phosphorylation | Uniprot | |
| S658 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| S675 | Phosphorylation | Uniprot | |
| S687 | Phosphorylation | Uniprot | |
| S697 | Phosphorylation | Uniprot | |
| T711 | Phosphorylation | Uniprot | |
| K728 | Ubiquitination | Uniprot | |
| K754 | Ubiquitination | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| K860 | Ubiquitination | Uniprot | |
| Y945 | Phosphorylation | Uniprot | |
| Y953 | Phosphorylation | Uniprot | |
| K988 | Ubiquitination | Uniprot |
Research Backgrounds
Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Sumoylated.
Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
Nucleus. Nucleus>PML body.
Note: Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
Interacts with histone H3-3B, PAX3 and PAX7 (By similarity). Interacts with histone H3.Y. Interacts with CCNA1, HIRIP3, NFU1/HIRIP5 and histone H2B. Part of a complex which includes ASF1A, CABIN1, histone H3.3, histone H4 and UBN1.
Belongs to the WD repeat HIR1 family.
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