PAF1 Antibody - #DF13474

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Product: PAF1 Antibody
Catalog: DF13474
Description: Rabbit polyclonal antibody to PAF1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 59kDa; 60kD(Calculated).
Uniprot: Q8N7H5
RRID: AB_2846493

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PAF1 Antibody detects endogenous levels of total PAF1.
RRID:
AB_2846493
Cite Format: Affinity Biosciences Cat# DF13474, RRID:AB_2846493.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

F23149 1; FLJ11123; hPAF1; PAF 1; PAF1; Paf1 RNA polymerase II associated factor homolog; PAF1_HUMAN; Pancreatic differentiation protein 2; PD 2; PD2; PD2 protein; RNA polymerase II associated factor homolog; RNA polymerase II-associated factor 1 homolog;

Immunogens

Immunogen:
Uniprot:

Q8N7H5(PAF1_HUMAN) >>Visit HPA database.

Gene(ID):
PAF1(54623)
Sequence:
MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMETEEKEAGGSDEEQEKGSSSEKEGSEDEHSGSESEREEGDRDEASDKSGSGEDESSEDEARAARDKEEIFGSDADSEDDADSDDEDRGQAQGGSDNDSDSGSNGGGQRSRSHSRSASPFPSGSEHSAQEDGSEAAASDSSEADSDSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N7H5 As Substrate

Site PTM Type Enzyme
K34 Sumoylation
K34 Ubiquitination
S38 Phosphorylation
T50 Phosphorylation
Y61 Phosphorylation
K62 Ubiquitination
T64 Phosphorylation
S65 Phosphorylation
K68 Ubiquitination
K71 Ubiquitination
K106 Ubiquitination
S117 Phosphorylation
K119 Acetylation
K119 Methylation
K119 Ubiquitination
K126 Ubiquitination
K133 Sumoylation
K133 Ubiquitination
Y136 Phosphorylation
Y144 Phosphorylation
S147 Phosphorylation
K150 Ubiquitination
K160 Ubiquitination
Y169 Phosphorylation
K170 Ubiquitination
K181 Ubiquitination
K188 Ubiquitination
S191 Phosphorylation
S195 Phosphorylation
K196 Ubiquitination
T200 Phosphorylation
K269 Ubiquitination
K272 Ubiquitination
K290 Ubiquitination
K300 Ubiquitination
K305 Ubiquitination
K338 Ubiquitination
T345 Phosphorylation
K352 Ubiquitination
K359 Ubiquitination
T386 Phosphorylation
S394 Phosphorylation
S409 Phosphorylation
S414 Phosphorylation
S416 Phosphorylation
S418 Phosphorylation
S429 Phosphorylation
S439 Phosphorylation
S440 Phosphorylation
S456 Phosphorylation
S460 Phosphorylation
S466 Phosphorylation
S484 Phosphorylation
S486 Phosphorylation
S493 Phosphorylation
S505 Phosphorylation
S510 Phosphorylation
S516 Phosphorylation
S521 Phosphorylation

Research Backgrounds

Function:

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro.

Subcellular Location:

Nucleus.
Note: Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. The PAF1 complex interacts with PHF5A (By similarity). Interacts with POLR2A, TCEA1, TTC37, KMT2A/MLL1, SUPT5H, RNF20 and RNF40. Interacts with UBE2E1. Interacts with influenza A strain H3N2 NS1 protein; the interaction interferes with host cell gene transcription, specifically with that of antiviral genes.

Family&Domains:

Belongs to the PAF1 family.

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