MAFK Antibody - #DF13483

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Product: MAFK Antibody
Catalog: DF13483
Description: Rabbit polyclonal antibody to MAFK
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Dog, Chicken
Mol.Wt.: 17kDa; 18kD(Calculated).
Uniprot: O60675
RRID: AB_2846502

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
MAFK Antibody detects endogenous levels of total MAFK.
RRID:
AB_2846502
Cite Format: Affinity Biosciences Cat# DF13483, RRID:AB_2846502.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Erythroid transcription factor NF E2 p18 subunit; Erythroid transcription factor NF-E2 p18 subunit; FLJ32205; MAFK; MAFK_HUMAN; MGC71717; Nfe2u; P18; Transcription factor MafK; V maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian);

Immunogens

Immunogen:

A synthesized peptide derived from human MAFK, corresponding to a region within N-terminal amino acids.

Uniprot:

O60675(MAFK_HUMAN) >>Visit HPA database.

Gene(ID):
MAFK(7975)
Sequence:
MTTNPKPNKALKVKKEAGENAPVLSDDELVSMSVRELNQHLRGLTKEEVTRLKQRRRTLKNRGYAASCRIKRVTQKEELERQRVELQQEVEKLARENSSMRLELDALRSKYEALQTFARTVARGPVAPSKVATTSVITIVKSTELSSTSVPFSAAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Chicken
100
Zebrafish
88
Horse
0
Sheep
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the bZIP family. Maf subfamily.

References

1). Physical Exercise Improves the Neuronal Function in Ischemic Stroke Via Microglial CB2R/P2Y12 Signaling. Molecular neurobiology, 2024 (PubMed: 39066973) [IF=4.6]
2). Ciliary neurotrophic factor promotes the development of homocysteine-induced vascular endothelial injury through inflammation mediated by the JAK2/STAT3 signaling pathway. Experimental cell research, 2024 (PubMed: 38848951) [IF=3.3]

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