eIF4ENIF1 Antibody - #DF13486

(3)  
Product: eIF4ENIF1 Antibody
Catalog: DF13486
Description: Rabbit polyclonal antibody to eIF4ENIF1
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 108kDa; 108kD(Calculated).
Uniprot: Q9NRA8
RRID: AB_2846505

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
eIF4ENIF1 Antibody detects endogenous levels of total eIF4ENIF1.
RRID:
AB_2846505
Cite Format: Affinity Biosciences Cat# DF13486, RRID:AB_2846505.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2610509L04Rik; 4E T; 4E-T; 4ET; 4ET_HUMAN; A930019J01Rik; AA410001; AU021239; Clast 4; Clast4; D11Ertd166e; eIF4E transporter; Eif4enif1; Eukaryotic translation initiation factor 4E nuclear import factor 1; Eukaryotic translation initiation factor 4E transporter; FLJ21601; FLJ26551; OTTMUSP00000000698; OTTMUSP00000005193; OTTMUSP00000005194; OTTMUSP00000036075;

Immunogens

Immunogen:
Uniprot:

Q9NRA8(4ET_HUMAN) >>Visit HPA database.

Gene(ID):
EIF4ENIF1(56478)
Expression:
Q9NRA8 4ET_HUMAN:

Widely expressed.

Sequence:
MDRRSMGETESGDAFLDLKKPPASKCPHRYTKEELLDIKELPHSKQRPSCLSEKYDSDGVWDPEKWHASLYPASGRSSPVESLKKELDTDRPSLVRRIVDPRERVKEDDLDVVLSPQRRSFGGGCHVTAAVSSRRSGSPLEKDSDGLRLLGGRRIGSGRIISARTFEKDHRLSDKDLRDLRDRDRERDFKDKRFRREFGDSKRVFGERRRNDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGRKRTRRRTASVKEGIVECNGGVAEEDEVEVILAQEPAADQEVPRDAVLPEQSPGDFDFNEFFNLDKVPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSRSSSLGSTPHEELERLAGLEQAILSPGQNSGNYFAPIPLEDHAENKVDILEMLQKAKVDLKPLLSSLSANKEKLKESSHSGVVLSVEEVEAGLKGLKVDQQVKNSTPFMAEHLEETLSAVTNNRQLKKDGDMTAFNKLVSTMKASGTLPSQPKVSRNLESHLMSPAEIPGQPVPKNILQELLGQPVQRPASSNLLSGLMGSLEPTTSLLGQRAPSPPLSQVFQTRAASADYLRPRIPSPIGFTPGPQQLLGDPFQGMRKPMSPITAQMSQLELQQAALEGLALPHDLAVQAANFYQPGFGKPQVDRTRDGFRNRQQRVTKSPAPVHRGNSSSPAPAASITSMLSPSFTPTSVIRKMYESKEKSKEEPASGKAALGDSKEDTQKASEENLLSSSSVPSADRDSSPTTNSKLSALQRSSCSTPLSQANRYTKEQDYRPKATGRKTPTLASPVPTTPFLRPVHQVPLVPHVPMVRPAHQLHPGLVQRMLAQGVHPQHLPSLLQTGVLPPGMDLSHLQGISGPILGQPFYPLPAASHPLLNPRPGTPLHLAMVQQQLQRSVLHPPGSGSHAAAVSVQTTPQNVPSRSGLPHMHSQLEHRPSQRSSSPVGLAKWFGSDVLQQPLPSMPAKVISVDELEYRQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NRA8 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
S24 Phosphorylation
Y30 Phosphorylation
T31 Phosphorylation
K32 Ubiquitination
K39 Ubiquitination
K54 Ubiquitination
Y71 Phosphorylation
S74 Phosphorylation
S77 Phosphorylation
S78 Phosphorylation
S82 Phosphorylation
K85 Ubiquitination
K106 Ubiquitination
S115 Phosphorylation P28482 (MAPK1)
S120 Phosphorylation
S136 Phosphorylation
S138 Phosphorylation
S144 Phosphorylation
S173 Phosphorylation
K202 Acetylation
S213 Phosphorylation
Y214 Phosphorylation
T215 Phosphorylation
S223 Phosphorylation
T227 Phosphorylation
T253 Phosphorylation
T257 Phosphorylation
S259 Phosphorylation
S301 Phosphorylation P45983 (MAPK8)
S321 Phosphorylation
S331 Phosphorylation
S342 Phosphorylation
S345 Phosphorylation
S351 Phosphorylation
S352 Phosphorylation
S353 Phosphorylation
S356 Phosphorylation
T357 Phosphorylation
S374 Phosphorylation P45983 (MAPK8)
S379 Phosphorylation
Y382 Phosphorylation
K404 Ubiquitination
K406 Ubiquitination
K410 Ubiquitination
S417 Phosphorylation
K420 Ubiquitination
K443 Ubiquitination
K446 Ubiquitination
K452 Ubiquitination
S454 Phosphorylation
T455 Phosphorylation
T465 Phosphorylation
S467 Phosphorylation
K477 Ubiquitination
K486 Acetylation
K486 Ubiquitination
K492 Methylation
K492 Ubiquitination
S494 Phosphorylation
T496 Phosphorylation
S499 Phosphorylation
K502 Ubiquitination
S509 Phosphorylation
S513 Phosphorylation P45983 (MAPK8)
S541 Phosphorylation
S564 Phosphorylation
S568 Phosphorylation
R574 Methylation
S577 Phosphorylation
Y580 Phosphorylation
R582 Methylation
R584 Methylation
S587 Phosphorylation P45983 (MAPK8)
T592 Phosphorylation
S611 Phosphorylation
T614 Phosphorylation
S618 Phosphorylation
S670 Phosphorylation
S680 Phosphorylation
S681 Phosphorylation
S693 Phosphorylation P45983 (MAPK8)
K732 Ubiquitination
S746 Phosphorylation
S751 Phosphorylation
S752 Phosphorylation P45983 (MAPK8)
T754 Phosphorylation
T755 Phosphorylation
S757 Phosphorylation
K758 Ubiquitination
S765 Phosphorylation
S766 Phosphorylation
S768 Phosphorylation
T769 Phosphorylation
S772 Phosphorylation
K779 Ubiquitination
T792 Phosphorylation
T891 Phosphorylation
S912 Phosphorylation
S914 Phosphorylation
S920 Phosphorylation
T924 Phosphorylation
S946 Phosphorylation
R948 Methylation
S951 Phosphorylation
S977 Phosphorylation
Y983 Phosphorylation

Research Backgrounds

Function:

Nucleoplasmic shuttling protein, which inhibits translation initiation. Mediates the nuclear import of EIF4E by a piggy-back mechanism.

Subcellular Location:

Cytoplasm. Nucleus. Nucleus>PML body. Nucleus speckle.
Note: Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Localization to nuclear foci and speckles requires active transcription.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with EIF4E. Interacts with importin beta only in the presence of importin alpha, suggesting a direct interaction with importin alpha. Interacts with APOBEC3G in an RNA-dependent manner.

Restrictive clause

 

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