p400 Antibody - #DF13490

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Product: p400 Antibody
Catalog: DF13490
Description: Rabbit polyclonal antibody to p400
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 343kDa; 343kD(Calculated).
Uniprot: Q96L91
RRID: AB_2846509

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Horse(88%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
p400 Antibody detects endogenous levels of total p400.
RRID:
AB_2846509
Cite Format: Affinity Biosciences Cat# DF13490, RRID:AB_2846509.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAG repeat protein 32; CAGH 32; CAGH32; DKFZP434I225; Domino homolog; E1A binding protein p400; E1A-binding protein p400; EP 400; EP400; EP400_HUMAN; FLJ42018; FLJ45115; hDomino; KIAA1498; KIAA1818; p400 kDa SWI2/SNF2 related protein; p400 kDa SWI2/SNF2-related protein; P400 SWI2/SNF2 related protein; TNRC 12; TNRC12; Trinucleotide repeat containing 12; Trinucleotide repeat containing gene 12 protein; Trinucleotide repeat-containing gene 12 protein;

Immunogens

Immunogen:
Uniprot:

Q96L91(EP400_HUMAN) >>Visit HPA database.

Gene(ID):
EP400(57634)
Expression:
Q96L91 EP400_HUMAN:

Ubiquitously expressed.

Sequence:
MHHGTGPQNVQHQLQRSRACPGSEGEEQPAHPNPPPSPAAPFAPSASPSAPQSPSYQIQQLMNRSPATGQNVNITLQSVGPVVGGNQQITLAPLPLPSPTSPGFQFSAQPRRFEHGSPSYIQVTSPLSQQVQTQSPTQPSPGPGQALQNVRAGAPGPGLGLCSSSPTGGFVDASVLVRQISLSPSSGGHFVFQDGSGLTQIAQGAQVQLQHPGTPITVRERRPSQPHTQSGGTIHHLGPQSPAAAGGAGLQPLASPSHITTANLPPQISSIIQGQLVQQQQVLQGPPLPRPLGFERTPGVLLPGAGGAAGFGMTSPPPPTSPSRTAVPPGLSSLPLTSVGNTGMKKVPKKLEEIPPASPEMAQMRKQCLDYHYQEMQALKEVFKEYLIELFFLQHFQGNMMDFLAFKKKHYAPLQAYLRQNDLDIEEEEEEEEEEEEKSEVINDEVKVVTGKDGQTGTPVAIATQLPPKVSAAFSSQQQPFQQALAGSLVAGAGSTVETDLFKRQQAMPSTGMAEQSKRPRLEVGHQGVVFQHPGADAGVPLQQLMPTAQGGMPPTPQAAQLAGQRQSQQQYDPSTGPPVQNAASLHTPLPQLPGRLPPAGVPTAALSSALQFAQQPQVVEAQTQLQIPVKTQQPNVPIPAPPSSQLPIPPSQPAQLALHVPTPGKVQVQASQLSSLPQMVASTRLPVDPAPPCPRPLPTSSTSSLAPVSGSGPGPSPARSSPVNRPSSATNKALSPVTSRTPGVVASAPTKPQSPAQNATSSQDSSQDTLTEQITLENQVHQRIAELRKAGLWSQRRLPKLQEAPRPKSHWDYLLEEMQWMATDFAQERRWKVAAAKKLVRTVVRHHEEKQLREERGKKEEQSRLRRIAASTAREIECFWSNIEQVVEIKLRVELEEKRKKALNLQKVSRRGKELRPKGFDALQESSLDSGMSGRKRKASISLTDDEVDDEEETIEEEEANEGVVDHQTELSNLAKEAELPLLDLMKLYEGAFLPSSQWPRPKPDGEDTSGEEDADDCPGDRESRKDLVLIDSLFIMDQFKAAERMNIGKPNAKDIADVTAVAEAILPKGSARVTTSVKFNAPSLLYGALRDYQKIGLDWLAKLYRKNLNGILADEAGLGKTVQIIAFFAHLACNEGNWGPHLVVVRSCNILKWELELKRWCPGLKILSYIGSHRELKAKRQEWAEPNSFHVCITSYTQFFRGLTAFTRVRWKCLVIDEMQRVKGMTERHWEAVFTLQSQQRLLLIDSPLHNTFLELWTMVHFLVPGISRPYLSSPLRAPSEESQDYYHKVVIRLHRVTQPFILRRTKRDVEKQLTKKYEHVLKCRLSNRQKALYEDVILQPGTQEALKSGHFVNVLSILVRLQRICNHPGLVEPRHPGSSYVAGPLEYPSASLILKALERDFWKEADLSMFDLIGLENKITRHEAELLSKKKIPRKLMEEISTSAAPAARPAAAKLKASRLFQPVQYGQKPEGRTVAFPSTHPPRTAAPTTASAAPQGPLRGRPPIATFSANPEAKAAAAPFQTSQASASAPRHQPASASSTAASPAHPAKLRAQTTAQASTPGQPPPQPQAPSHAAGQSALPQRLVLPSQAQARLPSGEVVKIAQLASITGPQSRVAQPETPVTLQFQGSKFTLSHSQLRQLTAGQPLQLQGSVLQIVSAPGQPYLRAPGPVVMQTVSQAGAVHGALGSKPPAGGPSPAPLTPQVGVPGRVAVNALAVGEPGTASKPASPIGGPTQEEKTRLLKERLDQIYLVNERRCSQAPVYGRDLLRICALPSHGRVQWRGSLDGRRGKEAGPAHSYTSSSESPSELMLTLCRCGESLQDVIDRVAFVIPPVVAAPPSLRVPRPPPLYSHRMRILRQGLREHAAPYFQQLRQTTAPRLLQFPELRLVQFDSGKLEALAILLQKLKSEGRRVLILSQMILMLDILEMFLNFHYLTYVRIDENASSEQRQELMRSFNRDRRIFCAILSTHSRTTGINLVEADTVVFYDNDLNPVMDAKAQEWCDRIGRCKDIHIYRLVSGNSIEEKLLKNGTKDLIREVAAQGNDYSMAFLTQRTIQELFEVYSPMDDAGFPVKAEEFVVLSQEPSVTETIAPKIARPFIEALKSIEYLEEDAQKSAQEGVLGPHTDALSSDSENMPCDEEPSQLEELADFMEQLTPIEKYALNYLELFHTSIEQEKERNSEDAVMTAVRAWEFWNLKTLQEREARLRLEQEEAELLTYTREDAYSMEYVYEDVDGQTEVMPLWTPPTPPQDDSDIYLDSVMCLMYEATPIPEAKLPPVYVRKERKRHKTDPSAAGRKKKQRHGEAVVPPRSLFDRATPGLLKIRREGKEQKKNILLKQQVPFAKPLPTFAKPTAEPGQDNPEWLISEDWALLQAVKQLLELPLNLTIVSPAHTPNWDLVSDVVNSCSRIYRSSKQCRNRYENVIIPREEGKSKNNRPLRTSQIYAQDENATHTQLYTSHFDLMKMTAGKRSPPIKPLLGMNPFQKNPKHASVLAESGINYDKPLPPIQVASLRAERIAKEKKALADQQKAQQPAVAQPPPPQPQPPPPPQQPPPPLPQPQAAGSQPPAGPPAVQPQPQPQPQTQPQPVQAPAKAQPAITTGGSAAVLAGTIKTSVTGTSMPTGAVSGNVIVNTIAGVPAATFQSINKRLASPVAPGALTTPGGSAPAQVVHTQPPPRAVGSPATATPDLVSMATTQGVRAVTSVTASAVVTTNLTPVQTPARSLVPQVSQATGVQLPGKTITPAHFQLLRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTTTTSQVQVPQIQGQAQSPAQIKAVGKLTPEHLIKMQKQKLQMPPQPPPPQAQSAPPQPTAQVQVQTSQPPQQQSPQLTTVTAPRPGALLTGTTVANLQVARLTRVPTSQLQAQGQMQTQAPQPAQVALAKPPVVSVPAAVVSSPGVTTLPMNVAGISVAIGQPQKAAGQTVVAQPVHMQQLLKLKQQAVQQQKAIQPQAAQGPAAVQQKITAQQITTPGAQQKVAYAAQPALKTQFLTTPISQAQKLAGAQQVQTQIQVAKLPQVVQQQTPVASIQQVASASQQASPQTVALTQATAAGQQVQMIPAVTATAQVVQQKLIQQQVVTTASAPLQTPGAPNPAQVPASSDSPSQQPKLQMRVPAVRLKTPTKPPCQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Dog
100
Pig
88
Horse
88
Bovine
88
Xenopus
57
Chicken
57
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96L91 As Substrate

Site PTM Type Enzyme
S37 Phosphorylation
S53 Phosphorylation
S117 Phosphorylation
S119 Phosphorylation
Y120 Phosphorylation
S125 Phosphorylation
S128 Phosphorylation
T133 Phosphorylation
S135 Phosphorylation
T137 Phosphorylation
S140 Phosphorylation
R151 Methylation
S164 Phosphorylation
S165 Phosphorylation
S181 Phosphorylation
S183 Phosphorylation
S185 Phosphorylation
S186 Phosphorylation
T214 Phosphorylation
T217 Phosphorylation
R219 Methylation
R221 Methylation
S224 Phosphorylation
T233 Phosphorylation
T314 Phosphorylation
S315 Phosphorylation
T320 Phosphorylation
S321 Phosphorylation
S323 Phosphorylation
K345 Acetylation
K346 Acetylation
S358 Phosphorylation
Y386 Phosphorylation
K409 Ubiquitination
S717 Phosphorylation
S721 Phosphorylation
S722 Phosphorylation
S728 Phosphorylation
S729 Phosphorylation
T731 Phosphorylation
K733 Acetylation
S736 Phosphorylation
T739 Phosphorylation
S755 Phosphorylation
S864 Phosphorylation
K899 Ubiquitination
K902 Ubiquitination
K908 Ubiquitination
K919 Ubiquitination
S928 Phosphorylation
S934 Phosphorylation
S941 Phosphorylation
S943 Phosphorylation
T945 Phosphorylation
T955 Phosphorylation
S973 Phosphorylation
T1010 Phosphorylation
S1011 Phosphorylation
K1070 Ubiquitination
K1096 Ubiquitination
K1108 Ubiquitination
K1122 Acetylation
S1190 Phosphorylation
T1209 Phosphorylation
S1275 Phosphorylation
S1276 Phosphorylation
K1291 Ubiquitination
K1319 Ubiquitination
K1333 Ubiquitination
K1398 Ubiquitination
K1421 Ubiquitination
S1431 Phosphorylation
K1432 Ubiquitination
K1438 Ubiquitination
K1457 Acetylation
K1459 Methylation
K1472 Acetylation
K1472 Ubiquitination
S1482 Phosphorylation
T1483 Phosphorylation
S1547 Phosphorylation
K1553 Acetylation
K1553 Ubiquitination
R1555 Methylation
T1559 Phosphorylation
T1564 Phosphorylation
R1597 Methylation
K1605 Ubiquitination
T1624 Phosphorylation
K1634 Acetylation
K1634 Ubiquitination
S1638 Phosphorylation
S1700 Phosphorylation
T1726 Phosphorylation
S1728 Phosphorylation
K1729 Ubiquitination
S1732 Phosphorylation
T1738 Phosphorylation
K1742 Ubiquitination
K1795 Ubiquitination
S1912 Phosphorylation
K2014 Ubiquitination
K2030 Ubiquitination
K2037 Ubiquitination
S2086 Phosphorylation
K2098 Ubiquitination
K2108 Ubiquitination
K2181 Ubiquitination
K2202 Ubiquitination
Y2284 Phosphorylation
K2293 Ubiquitination
T2294 Phosphorylation
S2297 Phosphorylation
T2322 Phosphorylation
K2342 Ubiquitination
K2349 Acetylation
T2353 Phosphorylation
K2356 Acetylation
K2356 Ubiquitination
T2471 Phosphorylation
S2476 Phosphorylation
K2480 Acetylation
K2493 Ubiquitination
S2608 Phosphorylation
T2615 Phosphorylation
S2656 Phosphorylation
T2665 Phosphorylation
S2686 Phosphorylation
T2691 Phosphorylation
S2696 Phosphorylation
S2734 Phosphorylation
T2747 Phosphorylation
T2813 Phosphorylation
K2970 Ubiquitination
T2996 Phosphorylation
T3002 Phosphorylation
Y3011 Phosphorylation
R3149 Methylation
T3152 Phosphorylation
K3155 Acetylation

Research Backgrounds

Function:

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. May also participate in the formation of NuA4 related complexes which lack the KAT5/TIP60 catalytic subunit, but which include the SWI/SNF related protein SRCAP. The NuA4 complex interacts with MYC and the adenovirus E1A protein. EP400 interacts with TRRAP, RUVBL1 and RUVBL2. Component of a SWR1-like complex. Interacts with ZNF42. Interacts with PHF5A. Interacts with human cytomegalovirus UL27. Interacts with human adenovirus 5 E1A protein; this interaction stabilizes MYC.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.

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