CASC5 Antibody - #DF13491

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Product: CASC5 Antibody
Catalog: DF13491
Description: Rabbit polyclonal antibody to CASC5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit
Mol.Wt.: 265kDa; 265kD(Calculated).
Uniprot: Q8NG31
RRID: AB_2846510

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Horse(88%), Rabbit(86%)
Clonality:
Polyclonal
Specificity:
CASC5 Antibody detects endogenous levels of total CASC5.
RRID:
AB_2846510
Cite Format: Affinity Biosciences Cat# DF13491, RRID:AB_2846510.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

;Protein D40/AF15q14; AF15Q14; ALL1 fused gene from chromosome 15q14; Blinkin bub linking kinetochore protein; Cancer susceptibility candidate 5; Cancer susceptibility candidate gene 5 protein; Cancer/testis antigen 29; CT29; D40; hKNL 1; hSpc105; KIAA1570; Kinetochore null 1 homolog; Kinetochore null protein 1; Kinetochore scaffold 1; KNL1;

Immunogens

Immunogen:
Uniprot:

Q8NG31(KNL1_HUMAN) >>Visit HPA database.

Gene(ID):
KNL1(57082)
Expression:
Q8NG31 KNL1_HUMAN:

Highly expressed in testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. Detected in the acrosome of ejaculated spermatozoa. Detected in adult thymus, bone marrow, colon, small intestine, appendix and placenta, and in fetal liver and thymus.

Sequence:
MDGVSSEANEENDNIERPVRRRHSSILKPPRSPLQDLRGGNERVQESNALRNKKNSRRVSFADTIKVFQTESHMKIVRKSEMEGCSAMVPSQLQLLPPGFKRFSCLSLPETETGENLLLIQNKKLEDNYCEITGMNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLTSSHTVMITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKIDFNDFIKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLFREKDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATGNFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMGAETHIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEKNLLKHDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQIAAAPTPEKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTELLSGENMDLTESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQRSDIIAKNSLTDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQITNRNTVSWEQSLFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSLSNPTPDYCHDKMIICSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMNRQIAVKVEKCGKSPIEKSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGPKIDKTIVFSEDDKNDMDITKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDMEITRSHTTALECKTVSPDEITTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDRPNFELSQRKSLGTPTVICTPTEESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEVADNMELSKSATCKNIKDVQSPGFLNEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDITQSCMVEIDNESALEDKEDFHLAGASKTILYSCGQDDMEITRSHTTALECKTLLPNEIAIRPMDKTVLFTDNYSDLEVTDSHTVFIDCQATEKILEENPKFGIGKGKNLGVSFPKDNSCVQEIAEKQALAVGNKIVLHTEQKQQLFAATNRTTNEIIKFHSAAMDEKVIGKVVDQACTLEKAQVESCQLNNRDRRNVDFTSSHATAVCGSSDNYSCLPNVISCTDNLEGSAMLLCDKDEEKANYCPVQNDLAYANDFASEYYLESEGQPLSAPCPLLEKEEVIQTSTKGQLDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMTKLNSKRVSFKLPKDQMKVYVDDIYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKSLNIIENSSAPICENKPKILNSEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQTHVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAHNDMHIVQATEIHNINIISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEENLPVYPDEINSSDSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKEKKIRKNEIKFSDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRSSQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNFTVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWEKMRHCSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDEMDKILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLLELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTITFEESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTCTTQHQLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAAFAKFEITLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQDLFQDCHFYH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Rabbit
86
Dog
75
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NG31 As Substrate

Site PTM Type Enzyme
S24 Phosphorylation Q96GD4 (AURKB)
S25 Phosphorylation
K28 Ubiquitination
S32 Phosphorylation
S60 Phosphorylation Q96GD4 (AURKB)
K75 Ubiquitination
S80 Phosphorylation
K124 Ubiquitination
Y129 Phosphorylation
K150 Ubiquitination
S153 Phosphorylation
K199 Ubiquitination
K202 Sumoylation
K202 Ubiquitination
K213 Acetylation
K213 Ubiquitination
K223 Acetylation
K223 Ubiquitination
K238 Ubiquitination
K246 Ubiquitination
K252 Sumoylation
K252 Ubiquitination
K262 Ubiquitination
Y270 Phosphorylation
K272 Ubiquitination
K288 Sumoylation
K288 Ubiquitination
S294 Phosphorylation
K303 Ubiquitination
K387 Ubiquitination
K393 Ubiquitination
S402 Phosphorylation
S405 Phosphorylation
K408 Ubiquitination
T438 Phosphorylation
Y443 Phosphorylation
S458 Phosphorylation
K467 Ubiquitination
K476 Ubiquitination
K480 Ubiquitination
K487 Ubiquitination
Y489 Phosphorylation
S497 Phosphorylation
T499 Phosphorylation
K501 Ubiquitination
S505 Phosphorylation
T513 Phosphorylation
K514 Ubiquitination
T517 Phosphorylation
K526 Sumoylation
K526 Ubiquitination
T539 Phosphorylation
K557 Ubiquitination
K570 Ubiquitination
S576 Phosphorylation
S578 Phosphorylation
S584 Phosphorylation
T586 Phosphorylation
K589 Ubiquitination
S633 Phosphorylation
S634 Phosphorylation
S635 Phosphorylation
T642 Phosphorylation
K643 Ubiquitination
K657 Sumoylation
K657 Ubiquitination
T663 Phosphorylation
K666 Ubiquitination
K668 Ubiquitination
K673 Ubiquitination
K680 Ubiquitination
S682 Phosphorylation
K707 Ubiquitination
K726 Ubiquitination
K737 Ubiquitination
K749 Ubiquitination
S750 Phosphorylation
S756 Phosphorylation
K757 Ubiquitination
K764 Ubiquitination
S765 Phosphorylation
S767 Phosphorylation
T770 Phosphorylation
Y773 Phosphorylation
K777 Ubiquitination
K810 Ubiquitination
K830 Acetylation
K830 Sumoylation
K830 Ubiquitination
S837 Phosphorylation
K841 Ubiquitination
K846 Ubiquitination
S847 Phosphorylation
S858 Phosphorylation
K861 Ubiquitination
K872 Ubiquitination
S878 Phosphorylation
K883 Ubiquitination
K886 Ubiquitination
T905 Phosphorylation
K916 Ubiquitination
T943 Phosphorylation
K953 Ubiquitination
S956 Phosphorylation
S1004 Phosphorylation
K1007 Ubiquitination
S1008 Phosphorylation
T1011 Phosphorylation
T1013 Phosphorylation
T1017 Phosphorylation
T1019 Phosphorylation
S1039 Phosphorylation
T1042 Phosphorylation
K1064 Ubiquitination
K1069 Ubiquitination
K1072 Sumoylation
K1072 Ubiquitination
S1076 Phosphorylation
S1085 Phosphorylation
S1086 Phosphorylation
K1087 Ubiquitination
S1088 Phosphorylation
S1093 Phosphorylation
K1100 Ubiquitination
T1155 Phosphorylation
K1165 Ubiquitination
K1214 Ubiquitination
K1221 Ubiquitination
K1229 Ubiquitination
S1232 Phosphorylation
K1240 Ubiquitination
K1248 Ubiquitination
K1256 Ubiquitination
T1263 Phosphorylation
K1272 Ubiquitination
K1281 Ubiquitination
K1285 Ubiquitination
K1295 Ubiquitination
K1393 Ubiquitination
K1402 Ubiquitination
K1413 Ubiquitination
K1419 Ubiquitination
Y1432 Phosphorylation
K1441 Ubiquitination
S1448 Phosphorylation
K1450 Ubiquitination
K1453 Ubiquitination
K1457 Ubiquitination
Y1459 Phosphorylation
Y1464 Phosphorylation
K1480 Ubiquitination
K1481 Ubiquitination
K1488 Ubiquitination
S1494 Phosphorylation
K1495 Ubiquitination
K1499 Ubiquitination
S1500 Phosphorylation
K1515 Sumoylation
K1515 Ubiquitination
K1517 Ubiquitination
K1530 Acetylation
K1530 Ubiquitination
K1534 Ubiquitination
K1584 Ubiquitination
K1642 Ubiquitination
S1652 Phosphorylation
S1660 Phosphorylation
K1670 Methylation
S1675 Phosphorylation
S1690 Phosphorylation
T1692 Phosphorylation
K1718 Ubiquitination
S1724 Phosphorylation
K1770 Ubiquitination
S1773 Phosphorylation
K1778 Ubiquitination
T1814 Phosphorylation
T1824 Phosphorylation
K1830 Ubiquitination
S1831 Phosphorylation
S1834 Phosphorylation
K1838 Ubiquitination
S1841 Phosphorylation
T1843 Phosphorylation
S1845 Phosphorylation
S1846 Phosphorylation
S1848 Phosphorylation
S1849 Phosphorylation
S1850 Phosphorylation
K1855 Ubiquitination
S1860 Phosphorylation
T1879 Phosphorylation
K1888 Ubiquitination
S1915 Phosphorylation
T1925 Phosphorylation
K1942 Ubiquitination
K1961 Ubiquitination
K1969 Ubiquitination
K1976 Ubiquitination
K1981 Ubiquitination
S1986 Phosphorylation
Y1996 Phosphorylation
K2001 Acetylation
K2009 Ubiquitination
K2016 Acetylation
K2016 Ubiquitination
K2022 Ubiquitination
K2033 Ubiquitination
K2037 Ubiquitination
K2043 Ubiquitination
K2047 Ubiquitination
S2076 Phosphorylation
K2083 Ubiquitination
K2089 Sumoylation
K2089 Ubiquitination
T2090 Phosphorylation
K2106 Ubiquitination
K2118 Ubiquitination
K2214 Ubiquitination
K2247 Ubiquitination
K2324 Ubiquitination
K2328 Ubiquitination

Research Backgrounds

Function:

Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.

Subcellular Location:

Nucleus. Chromosome>Centromere>Kinetochore.
Note: Weakly expressed in interphase nuclei. Expression increases from prophase to late anaphase, but greatly diminishes from the telophase and cytokinesis to early G1 phase of cell cycle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. Detected in the acrosome of ejaculated spermatozoa. Detected in adult thymus, bone marrow, colon, small intestine, appendix and placenta, and in fetal liver and thymus.

Subunit Structure:

Interacts with DSN1, MIS12, BUB1, BUB1B, NSL1 and ZWINT.

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