SEC16A Antibody - #DF13492

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Product: SEC16A Antibody
Catalog: DF13492
Description: Rabbit polyclonal antibody to SEC16A
Application: WB
Reactivity: Human
Prediction: Bovine, Sheep, Dog
Mol.Wt.: 233kDa; 252kD(Calculated).
Uniprot: O15027
RRID: AB_2846511

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(88%), Sheep(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SEC16A Antibody detects endogenous levels of total SEC16A.
RRID:
AB_2846511
Cite Format: Affinity Biosciences Cat# DF13492, RRID:AB_2846511.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ26737; KIAA0310; OTTHUMP00000022589; OTTHUMP00000022592; OTTHUMP00000022593; p250; Protein SEC16 homolog A; Protein transport protein Sec16A; RP11 413M3.10; SEC 16; SEC 16A; SEC16; SEC16 homolog A (S. cerevisiae); SEC16 homolog A; SEC16L;

Immunogens

Immunogen:
Uniprot:

O15027(SC16A_HUMAN) >>Visit HPA database.

Gene(ID):
SEC16A(9919)
Expression:
O15027 SC16A_HUMAN:

Ubiquitous. Expressed at higher levels in the pancreas.

Sequence:
MQPPPQTVPSGMAGPPPAGNPRSVFWASSPYRRRANNNAAVAPTTCPLQPVTDPFAFSRQALQSTPLGSSSKSSPPVLQGPAPAGFSQHPGLLVPHTHARDSSQGPCEPLPGPLTQPRAHASPFSGALTPSAPPGPEMNRSAEVGPSSEPEVQTLPYLPHYIPGVDPETSHGGHPHGNMPGLDRPLSRQNPHDGVVTPAASPSLPQPGLQMPGQWGPVQGGPQPSGQHRSPCPEGPVPSGVPCATSVPHFPTPSILHQGPGHEQHSPLVAPPAALPSDGRDEVSHLQSGSHLANNSDPESTFRQNPRIVNHWASPELRQNPGVKNEHRPASALVNPLARGDSPENRTHHPLGAGAGSGCAPLEADSGASGALAMFFQGGETENEENLSSEKAGLSGQADFDDFCSSPGLGRPPAPTHVGAGSLCQALLPGPSNEAAGDVWGDTASTGVPDASGSQYENVENLEFVQNQEVLPSEPLNLDPSSPSDQFRYGPLPGPAVPRHGAVCHTGAPDATLHTVHPDSVSSSYSSRSHGRLSGSARPQELVGTFIQQEVGKPEDEASGSFFKQIDSSPVGGETDETTVSQNYRGSVSQPSTPSPPKPTGIFQTSANSSFEPVKSHLVGVKPFEADRANVVGEVRETCVRQKQCRPAAALPDASPGNLEQPPDNMETLCAPQVCPLPLNSTTEAVHMLPHAGAPPLDTVYPAPEKRPSARTQGPVKCESPATTLWAQSELPDFGGNVLLAPAAPALYVCAKPQPPVVQPPEEAMSGQQSRNPSSAAPVQSRGGIGASENLENPPKMGEEEALQSQASSGYASLLSSPPTESLQNPPVLIAQPDHSYNLAQPINFSVSLSNSHEKNQSWREALVGDRPAVSSWALGGDSGENTSLSGIPTSSVLSLSLPSSVAQSNFPQGSGASEMVSNQPANLLVQPPSQPVPENLVPESQKDRKAGSALPGFANSPAGSTSVVLVPPAHGTLVPDGNKANHSSHQEDTYGALDFTLSRTLENPVNVYNPSHSDSLASQQSVASHPRQSGPGAPNLDRFYQQVTKDAQGQPGLERAQQELVPPQQQASPPQLPKAMFSELSNPESLPAQGQAQNSAQSPASLVLVDAGQQLPPRPPQSSSVSLVSSGSGQAAVPSEQPWPQPVPALAPGPPPQDLAAYYYYRPLYDAYQPQYSLPYPPEPGAASLYYQDVYSLYEPRYRPYDGAASAYAQNYRYPEPERPSSRASHSSERPPPRQGYPEGYYSSKSGWSSQSDYYASYYSSQYDYGDPGHWDRYHYSARVRDPRTYDRRYWCDAEYDAYRREHSAFGDRPEKRDNNWRYDPRFTGSFDDDPDPHRDPYGEEVDRRSVHSEHSARSLHSAHSLASRRSSLSSHSHQSQIYRSHNVAAGSYEAPLPPGSFHGDFAYGTYRSNFSSGPGFPEYGYPADTVWPAMEQVSSRPTSPEKFSVPHVCARFGPGGQLIKVIPNLPSEGQPALVEVHSMEALLQHTSEQEEMRAFPGPLAKDDTHKVDVINFAQNKAMKCLQNENLIDKESASLLWNFIVLLCRQNGTVVGTDIAELLLRDHRTVWLPGKSPNEANLIDFTNEAVEQVEEEESGEAQLSFLTGGPAAAASSLERETERFRELLLYGRKKDALESAMKNGLWGHALLLASKMDSRTHARVMTRFANSLPINDPLQTVYQLMSGRMPAASTCCGDEKWGDWRPHLAMVLSNLNNNMDVESRTMATMGDTLASRGLLDAAHFCYLMAQAGFGVYTKKTTKLVLIGSNHSLPFLKFATNEAIQRTEAYEYAQSLGAETCPLPSFQVFKFIYSCRLAEMGLATQAFHYCEAIAKSILTQPHLYSPVLISQLVQMASQLRLFDPQLKEKPEEESLAAPTWLVHLQQVERQIKEGAGVWHQDGALPQQCPGTPSSEMEQLDRPGLSQPGALGIANPLLAVPAPSPEHSSPSVRLLPSAPQTLPDGPLASPARVPMFPVPLPPGPLEPGPGCVTPGPALGFLEPSGPGLPPGVPPLQERRHLLQEARSPDPGIVPQEAPVGNSLSELSEENFDGKFANLTPSRTVPDSEAPPGWDRADSGPTQPPLSLSPAPETKRPGQAAKKETKEPKKGESWFFRWLPGKKKTEAYLPDDKNKSIVWDEKKNQWVNLNEPEEEKKAPPPPPTSMPKTVQAAPPALPGPPGAPVNMYSRRAAGTRARYVDVLNPSGTQRSEPALAPADFVAPLAPLPIPSNLFVPTPDAEEPQLPDGTGREGPAAARGLANPEPAPEPKVLSSAASLPGSELPSSRPEGSQGGELSRCSSMSSLSREVSQHFNQAPGDLPAAGGPPSGAMPFYNPAQLAQACATSGSSRLGRIGQRKHLVLN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Bovine
88
Sheep
88
Horse
75
Xenopus
50
Chicken
50
Pig
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15027 As Substrate

Site PTM Type Enzyme
S29 Phosphorylation
Y31 Phosphorylation
S64 Phosphorylation
T65 Phosphorylation
S74 Phosphorylation
S122 Phosphorylation
T129 Phosphorylation
Y157 Phosphorylation
Y161 Phosphorylation
S187 Phosphorylation
T197 Phosphorylation
S201 Phosphorylation
S203 Phosphorylation
R229 Methylation
S288 Phosphorylation
S290 Phosphorylation
S296 Phosphorylation
T301 Phosphorylation
S314 Phosphorylation
R318 Methylation
S331 Phosphorylation
S342 Phosphorylation
S405 Phosphorylation
S482 Phosphorylation
Y489 Phosphorylation
S520 Phosphorylation
S523 Phosphorylation
S524 Phosphorylation
Y525 Phosphorylation
S526 Phosphorylation
S527 Phosphorylation
S529 Phosphorylation
S534 Phosphorylation
S536 Phosphorylation
T545 Phosphorylation
S559 Phosphorylation
S561 Phosphorylation
S568 Phosphorylation
S569 Phosphorylation
T575 Phosphorylation
Y584 Phosphorylation
S587 Phosphorylation
S589 Phosphorylation
S592 Phosphorylation
T593 Phosphorylation
S595 Phosphorylation
T600 Phosphorylation
S610 Phosphorylation
K622 Acetylation
K622 Ubiquitination
S720 Phosphorylation
Y748 Phosphorylation
S770 Phosphorylation
S774 Phosphorylation
S775 Phosphorylation
R782 Methylation
S846 Phosphorylation
S957 Phosphorylation
S961 Phosphorylation
T962 Phosphorylation
S984 Phosphorylation
S985 Phosphorylation
Y991 Phosphorylation
T997 Phosphorylation
Y1009 Phosphorylation
S1014 Phosphorylation
S1016 Phosphorylation
S1019 Phosphorylation
S1022 Phosphorylation
S1030 Phosphorylation
Y1041 Phosphorylation
K1046 Ubiquitination
S1069 Phosphorylation
S1079 Phosphorylation
S1174 Phosphorylation
Y1188 Phosphorylation
Y1192 Phosphorylation
S1193 Phosphorylation
Y1195 Phosphorylation
Y1199 Phosphorylation
Y1202 Phosphorylation
S1207 Phosphorylation
Y1209 Phosphorylation
Y1213 Phosphorylation
R1214 Methylation
Y1215 Phosphorylation
R1220 Methylation
S1222 Phosphorylation
S1223 Phosphorylation
S1226 Phosphorylation
S1229 Phosphorylation
Y1242 Phosphorylation
Y1243 Phosphorylation
S1244 Phosphorylation
S1250 Phosphorylation
S1261 Phosphorylation
Y1275 Phosphorylation
Y1277 Phosphorylation
S1278 Phosphorylation
Y1291 Phosphorylation
Y1297 Phosphorylation
Y1300 Phosphorylation
S1305 Phosphorylation
K1313 Ubiquitination
Y1320 Phosphorylation
T1325 Phosphorylation
S1327 Phosphorylation
S1347 Phosphorylation
S1350 Phosphorylation
S1353 Phosphorylation
S1356 Phosphorylation
S1359 Phosphorylation
S1362 Phosphorylation
S1365 Phosphorylation
S1368 Phosphorylation
S1369 Phosphorylation
S1371 Phosphorylation
S1372 Phosphorylation
S1374 Phosphorylation
S1377 Phosphorylation
Y1380 Phosphorylation
S1382 Phosphorylation
S1389 Phosphorylation
Y1390 Phosphorylation
S1398 Phosphorylation
Y1405 Phosphorylation
S1410 Phosphorylation
S1413 Phosphorylation
S1414 Phosphorylation
T1427 Phosphorylation
S1436 Phosphorylation
S1437 Phosphorylation
T1440 Phosphorylation
S1441 Phosphorylation
S1446 Phosphorylation
K1462 Sumoylation
K1462 Ubiquitination
K1503 Ubiquitination
K1508 Ubiquitination
K1518 Ubiquitination
K1521 Ubiquitination
S1573 Phosphorylation
T1583 Phosphorylation
S1601 Phosphorylation
Y1627 Phosphorylation
T1754 Phosphorylation
K1773 Ubiquitination
S1801 Phosphorylation
S1810 Phosphorylation
S1841 Phosphorylation
K1865 Ubiquitination
T1907 Phosphorylation
S1939 Phosphorylation
S1946 Phosphorylation
T1956 Phosphorylation
S1964 Phosphorylation
T1988 Phosphorylation
S2022 Phosphorylation
S2037 Phosphorylation
S2039 Phosphorylation
S2042 Phosphorylation
T2054 Phosphorylation
S2056 Phosphorylation
S2073 Phosphorylation
T2076 Phosphorylation
S2081 Phosphorylation
S2083 Phosphorylation
K2097 Acetylation
T2099 Phosphorylation
K2103 Acetylation
K2104 Acetylation
S2107 Phosphorylation
Y2122 Phosphorylation
K2129 Ubiquitination
S2130 Phosphorylation
K2137 Ubiquitination
T2158 O-Glycosylation
Y2182 Phosphorylation
S2183 Phosphorylation
Y2193 Phosphorylation
T2231 Phosphorylation
S2271 Phosphorylation
S2275 Phosphorylation
S2279 Phosphorylation
S2285 Phosphorylation
S2291 Phosphorylation
S2295 Phosphorylation
S2297 Phosphorylation
S2298 Phosphorylation
S2300 Phosphorylation
S2304 Phosphorylation
T2339 Phosphorylation
S2340 Phosphorylation
S2342 Phosphorylation

Research Backgrounds

Function:

Acts as a molecular scaffold that plays a key role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. Mediates the recruitment of MIA3/TANGO to ERES. Regulates both conventional (ER/Golgi-dependent) and GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane. Positively regulates the protein stability of E3 ubiquitin-protein ligases RNF152 and RNF183 and the ER localization of RNF183. Acts as a RAB10 effector in the regulation of insulin-induced SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the cell membrane in adipocytes (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein. Cytoplasm>Perinuclear region. Cytoplasm>Cytosol. Microsome membrane.
Note: SAR1A activity is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation (PubMed:17192411). Localizes to endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). MIA3 and LRRK2 are required for its proper localization to ERES (PubMed:25201882, PubMed:28442536, PubMed:19638414, PubMed:17428803, PubMed:22355596). Recruited to microsomal membrane in SAR1-dependent manner (PubMed:17428803).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Expressed at higher levels in the pancreas.

Subunit Structure:

SEC16A and SEC16B are each present in multiple copies in a heteromeric complex. Interacts with SEC23A. Interacts with RNF183 and RNF152. Interacts with LRRK2 (via ROC domain). Interacts with SEC13. Interacts with RAB10 (By similarity). Interacts with MIA3. Interacts with GORASP2 in response to ER stress.

Family&Domains:

Belongs to the SEC16 family.

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