PURA Antibody - #DF13493

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Product: PURA Antibody
Catalog: DF13493
Description: Rabbit polyclonal antibody to PURA
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Rabbit, Dog
Mol.Wt.: 34kDa, 42 kDa; 35kD(Calculated).
Uniprot: Q00577
RRID: AB_2846512

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PURA Antibody detects endogenous levels of total PURA.
RRID:
AB_2846512
Cite Format: Affinity Biosciences Cat# DF13493, RRID:AB_2846512.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PUR ALPHA; PUR1; purA; PURA_HUMAN; PURALPHA; Purine rich element binding protein A; Purine rich single stranded DNA binding protein alpha; Purine-rich single-stranded DNA-binding protein alpha; Transcriptional activator protein PUR alpha; Transcriptional activator protein Pur-alpha;

Immunogens

Immunogen:
Uniprot:

Q00577(PURA_HUMAN) >>Visit HPA database.

Gene(ID):
PURA(5813)
Sequence:
MADRDSGSEQGGAALGSGGSLGHPGSGSGSGGGGGGGGGGGGSGGGGGGAPGGLQHETQELASKRVDIQNKRFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDLAQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGVEEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPYKVWAKFGHTFCKYSEEMKKIQEKQREKRAACEQLHQQQQQQQEETAAATLLLQGEEEGEED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Pig
0
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q00577 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S6 Phosphorylation
S8 Phosphorylation
S26 Phosphorylation
S28 Phosphorylation
S30 Phosphorylation
S43 Phosphorylation
T58 Phosphorylation
S63 Phosphorylation
K71 Ubiquitination
Y74 Phosphorylation
K87 Ubiquitination
K97 Ubiquitination
S98 Phosphorylation
S127 Phosphorylation
K144 Ubiquitination
S145 Phosphorylation
K154 Methylation
Y156 Phosphorylation
K160 Methylation
T173 Phosphorylation
S182 Phosphorylation
T183 Phosphorylation
T187 Phosphorylation
T222 Phosphorylation
S223 Phosphorylation
K229 Ubiquitination
K239 Ubiquitination
Y240 Phosphorylation
K250 Ubiquitination
T252 Phosphorylation
Y253 Phosphorylation
S256 Phosphorylation
Y261 Phosphorylation
K262 Acetylation
K262 Ubiquitination
K266 Ubiquitination
K273 Acetylation
K273 Ubiquitination
Y274 Phosphorylation

Research Backgrounds

Function:

This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer, heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. Interacts with FMR1; this interaction occurs in association with polyribosome.

Family&Domains:

Belongs to the PUR DNA-binding protein family.

Restrictive clause

 

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For Research Use Only.
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