PARG Antibody - #DF13517
| Product: | PARG Antibody |
| Catalog: | DF13517 |
| Description: | Rabbit polyclonal antibody to PARG |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 111kDa, 70 kDa; 111kD(Calculated). |
| Uniprot: | Q86W56 |
| RRID: | AB_2846536 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13517, RRID:AB_2846536.
Fold/Unfold
ADP riboseglycohydrolase; Mitochondrial poly(ADP ribose) glycohydrolase; Parg; PARG_HUMAN; PARG99; Poly; poly (ADP ribose) glycohydrolase; poly(ADP-ribose) glycohydrolase 60 kDa isoform; Poly(ADP-ribose) glycohydrolase;
Immunogens
- Q86W56 PARG_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNAGPGCEPCTKRPRWGAATTSPAASDARSFPSRQRRVLDPKDAHVQFRVPPSSPACVPGRAGQHRGSATSLVFKQKTITSWMDTKGIKTAESESLDSKENNNTRIESMMSSVQKDNFYQHNVEKLENVSQLSLDKSPTEKSTQYLNQHQTAAMCKWQNEGKHTEQLLESEPQTVTLVPEQFSNANIDRSPQNDDHSDTDSEENRDNQQFLTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNKLTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDADIEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKAEDRRKEQWETKHQRTERKIPKYVPPHLSPDKKWLGTPIEEMRRMPRCGIRLPLLRPSANHTVTIRVDLLRAGEVPKPFPTHYKDLWDNKHVKMPCSEQNLYPVEDENGERTAGSRWELIQTALLNKFTRPQNLKDAILKYNVAYSKKWDFTALIDFWDKVLEEAEAQHLYQSILPDMVKIALCLPNICTQPIPLLKQKMNHSITMSQEQIASLLANAFFCTFPRRNAKMKSEYSSYPDINFNRLFEGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQSLEDFPEWERCEKPLTRLHVTYEGTIEENGQGMLQVDFANRFVGGGVTSAGLVQEEIRFLINPELIISRLFTEVLDHNECLIITGTEQYSEYTGYAETYRWSRSHEDGSERDDWQRRCTEIVAIDALHFRRYLDQFVPEKMRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYFTFGDSELMRDIYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCSTPGPDIKLYPFIYHAVESCAETADHSGQRTGT
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86W56 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T21 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| R66 | Methylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| K75 | Methylation | Uniprot | |
| K77 | Methylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| Y119 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| T139 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| Y442 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| K503 | Acetylation | Uniprot | |
| K509 | Ubiquitination | Uniprot | |
| T541 | Phosphorylation | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| T548 | Phosphorylation | Uniprot | |
| K554 | Ubiquitination | Uniprot | |
| K616 | Methylation | Uniprot | |
| S632 | Phosphorylation | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| Y656 | Phosphorylation | Uniprot | |
| Y912 | Phosphorylation | Uniprot | |
| S913 | Phosphorylation | Uniprot | |
| T919 | Phosphorylation | Uniprot | |
| T945 | Phosphorylation | Uniprot |
Research Backgrounds
Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose). PARG acts both as an endo- and exoglycosidase, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers. It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated. Poly(ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG. Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress. Required for retinoid acid-dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters. Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming.
Nucleus.
Note: Colocalizes with PCNA at replication foci (PubMed:21398629). Relocalizes to the cytoplasm in response to DNA damage (PubMed:16460818).
Cytoplasm.
Note: Translocates to the nucleus in response to DNA damage.
Cytoplasm.
Cytoplasm. Mitochondrion.
Mitochondrion matrix.
Ubiquitously expressed.
Interacts with PCNA. Interacts with NUDT5.
The PIP-box mediates interaction with PCNA and localization to replication foci.
Belongs to the poly(ADP-ribose) glycohydrolase family.
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