PARG Antibody - #DF13517

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Product: PARG Antibody
Catalog: DF13517
Description: Rabbit polyclonal antibody to PARG
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 111kDa, 70 kDa; 111kD(Calculated).
Uniprot: Q86W56
RRID: AB_2846536

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PARG Antibody detects endogenous levels of total PARG.
RRID:
AB_2846536
Cite Format: Affinity Biosciences Cat# DF13517, RRID:AB_2846536.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP riboseglycohydrolase; Mitochondrial poly(ADP ribose) glycohydrolase; Parg; PARG_HUMAN; PARG99; Poly; poly (ADP ribose) glycohydrolase; poly(ADP-ribose) glycohydrolase 60 kDa isoform; Poly(ADP-ribose) glycohydrolase;

Immunogens

Immunogen:
Uniprot:

Q86W56(PARG_HUMAN) >>Visit HPA database.

Gene(ID):
PARG(670)
Expression:
Q86W56 PARG_HUMAN:

Ubiquitously expressed.

Sequence:
MNAGPGCEPCTKRPRWGAATTSPAASDARSFPSRQRRVLDPKDAHVQFRVPPSSPACVPGRAGQHRGSATSLVFKQKTITSWMDTKGIKTAESESLDSKENNNTRIESMMSSVQKDNFYQHNVEKLENVSQLSLDKSPTEKSTQYLNQHQTAAMCKWQNEGKHTEQLLESEPQTVTLVPEQFSNANIDRSPQNDDHSDTDSEENRDNQQFLTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNKLTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDADIEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKAEDRRKEQWETKHQRTERKIPKYVPPHLSPDKKWLGTPIEEMRRMPRCGIRLPLLRPSANHTVTIRVDLLRAGEVPKPFPTHYKDLWDNKHVKMPCSEQNLYPVEDENGERTAGSRWELIQTALLNKFTRPQNLKDAILKYNVAYSKKWDFTALIDFWDKVLEEAEAQHLYQSILPDMVKIALCLPNICTQPIPLLKQKMNHSITMSQEQIASLLANAFFCTFPRRNAKMKSEYSSYPDINFNRLFEGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQSLEDFPEWERCEKPLTRLHVTYEGTIEENGQGMLQVDFANRFVGGGVTSAGLVQEEIRFLINPELIISRLFTEVLDHNECLIITGTEQYSEYTGYAETYRWSRSHEDGSERDDWQRRCTEIVAIDALHFRRYLDQFVPEKMRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYFTFGDSELMRDIYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCSTPGPDIKLYPFIYHAVESCAETADHSGQRTGT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Chicken
100
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
88
Xenopus
63
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86W56 As Substrate

Site PTM Type Enzyme
T21 Phosphorylation
S22 Phosphorylation
S54 Phosphorylation
R66 Methylation
S68 Phosphorylation
K75 Methylation
K77 Methylation
S112 Phosphorylation
K115 Ubiquitination
Y119 Phosphorylation
S130 Phosphorylation
S133 Phosphorylation
S137 Phosphorylation
T139 Phosphorylation
S190 Phosphorylation
S197 Phosphorylation
T199 Phosphorylation
S201 Phosphorylation
S240 Phosphorylation
S261 Phosphorylation
S264 Phosphorylation
S268 Phosphorylation
S286 Phosphorylation
S291 Phosphorylation
S298 Phosphorylation
S302 Phosphorylation
S316 Phosphorylation
S323 Phosphorylation
S375 Phosphorylation
K385 Ubiquitination
K431 Ubiquitination
Y442 Phosphorylation
S448 Phosphorylation
K503 Acetylation
K509 Ubiquitination
T541 Phosphorylation
K546 Ubiquitination
T548 Phosphorylation
K554 Ubiquitination
K616 Methylation
S632 Phosphorylation
K650 Ubiquitination
Y656 Phosphorylation
Y912 Phosphorylation
S913 Phosphorylation
T919 Phosphorylation
T945 Phosphorylation

Research Backgrounds

Function:

Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose). PARG acts both as an endo- and exoglycosidase, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers. It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated. Poly(ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG. Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress. Required for retinoid acid-dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters. Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming.

Subcellular Location:

Nucleus.
Note: Colocalizes with PCNA at replication foci (PubMed:21398629). Relocalizes to the cytoplasm in response to DNA damage (PubMed:16460818).

Cytoplasm.
Note: Translocates to the nucleus in response to DNA damage.

Cytoplasm.

Cytoplasm. Mitochondrion.

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with PCNA. Interacts with NUDT5.

Family&Domains:

The PIP-box mediates interaction with PCNA and localization to replication foci.

Belongs to the poly(ADP-ribose) glycohydrolase family.

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