TIMM50 Antibody - #DF13524
| Product: | TIMM50 Antibody |
| Catalog: | DF13524 |
| Description: | Rabbit polyclonal antibody to TIMM50 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 39kDa; 40kD(Calculated). |
| Uniprot: | Q3ZCQ8 |
| RRID: | AB_2846543 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13524, RRID:AB_2846543.
Fold/Unfold
Homolog of yeast Tim50; Import inner membrane translocase subunit TIM 50; Import inner membrane translocase subunit TIM50; Import inner membrane translocase subunit TIM50 mitochondrial; MGC102733; Mitochondrial import inner membrane translocase subunit TIM50; TIM 50; TIM 50L; TIM50; Tim50 like protein; TIM50_HUMAN; TIM50L; TIMM 50; TIMM50; translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae); Translocase of inner mitochondrial membrane 50 homolog (yeast); Translocase of inner mitochondrial membrane 50 homolog; Translocase of inner mitochondrial membrane 50, yeast, homolog of;
Immunogens
Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
- Q3ZCQ8 TIM50_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASAAVFSRLRSGLRLGSRGLCTRLATPPRRAPDQAAEIGSRGSTKAQGPQQQPGSEGPSYAKKVALWLAGLLGAGGTVSVVYIFGNNPVDENGAKIPDEFDNDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLQEPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMDGHHVKDISCLNRDPARVVVVDCKKEAFRLQPYNGVALRPWDGNSDDRVLLDLSAFLKTIALNGVEDVRTVLEHYALEDDPLAAFKQRQSRLEQEEQQRLAELSKSNKQNLFLGSLTSRLWPRSKQP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q3ZCQ8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| R43 | Methylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| Y62 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| C249 | S-Nitrosylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| S341 | Phosphorylation | Uniprot |
Research Backgrounds
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.
May participate in the release of snRNPs and SMN from the Cajal body.
Mitochondrion inner membrane>Single-pass membrane protein.
Nucleus speckle.
Note: Nuclear and enriched in speckles with snRNPs.
Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly interacts with TIMM23. The complex interacts with the TIMM44 component of the PAM complex and with DNAJC15.
Interacts with COIL and snRNPs.
The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
Belongs to the TIM50 family.
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