Product: Aurora A Antibody
Catalog: AF6011
Description: Rabbit polyclonal antibody to Aurora A
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 45kDa; 46kD(Calculated).
Uniprot: O14965
RRID: AB_2834945

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(100%)
Aurora A Antibody detects endogenous levels of total Aurora A.
Cite Format: Affinity Biosciences Cat# AF6011, RRID:AB_2834945.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


AIK; ARK-1; ARK1; AURA; Aurka; Aurora 2; Aurora A; Aurora kinase A; Aurora-related kinase 1; Aurora/IPL1 like kinase; Aurora/IPL1-related kinase 1; AURORA2; Breast tumor-amplified kinase; BTAK; hARK1; IAK; IPL1 related kinase; MGC34538; OTTHUMP00000031340; OTTHUMP00000031341; OTTHUMP00000031342; OTTHUMP00000031343; OTTHUMP00000031344; OTTHUMP00000031345; OTTHUMP00000166071; OTTHUMP00000166072; PPP1R47; Protein phosphatase 1, regulatory subunit 47; Serine/threonine kinase 15; Serine/threonine kinase 6; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; STK15; STK6; STK6_HUMAN; STK7;



Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14965 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S10 Phosphorylation O14965 (AURKA)
T16 Phosphorylation O14965 (AURKA)
K23 Ubiquitination
S41 Phosphorylation O14965 (AURKA)
S51 Phosphorylation
S53 Phosphorylation
S54 Phosphorylation
K63 Ubiquitination
S66 Phosphorylation
S67 Phosphorylation O14965 (AURKA)
K69 Ubiquitination
S83 Phosphorylation O14965 (AURKA)
S89 Phosphorylation
S98 Phosphorylation O14965 (AURKA)
K99 Ubiquitination
S104 Phosphorylation O14965 (AURKA)
S116 Phosphorylation
K117 Ubiquitination
K141 Ubiquitination
K143 Ubiquitination
Y148 Phosphorylation P12931 (SRC) , O14965 (AURKA)
S226 Phosphorylation O14965 (AURKA)
K227 Ubiquitination
K250 Ubiquitination
K258 Sumoylation
K258 Ubiquitination
S266 Phosphorylation O14965 (AURKA)
S278 Phosphorylation O14965 (AURKA)
S283 Phosphorylation
S284 Phosphorylation
T287 Phosphorylation Q05513 (PRKCZ) , O14965 (AURKA)
T288 Phosphorylation Q13153 (PAK1) , O14965 (AURKA) , P17612 (PRKACA)
T292 Phosphorylation
Y295 Phosphorylation
Y334 Phosphorylation
S342 Phosphorylation O14965 (AURKA) , Q13153 (PAK1)
S369 Phosphorylation
S391 Phosphorylation O14965 (AURKA)

PTMs - O14965 As Enzyme

Substrate Site Source
O14777 (NDC80) S55 Uniprot
O14965 (AURKA) S10 Uniprot
O14965 (AURKA) T16 Uniprot
O14965 (AURKA) S41 Uniprot
O14965 (AURKA) S67 Uniprot
O14965 (AURKA) S83 Uniprot
O14965 (AURKA) S98 Uniprot
O14965 (AURKA) S104 Uniprot
O14965 (AURKA) Y148 Uniprot
O14965 (AURKA) S226 Uniprot
O14965 (AURKA) S266 Uniprot
O14965 (AURKA) S278 Uniprot
O14965 (AURKA) T287 Uniprot
O14965 (AURKA) T288 Uniprot
O14965 (AURKA) S342 Uniprot
O14965 (AURKA) S391 Uniprot
O15143 (ARPC1B) T21 Uniprot
O15350 (TP73) S235 Uniprot
O43521 (BCL2L11) S93 Uniprot
O43521 (BCL2L11) S94 Uniprot
O43521 (BCL2L11) S98 Uniprot
O75496 (GMNN) T25 Uniprot
O95983 (MBD3) S24 Uniprot
O95983 (MBD3) S85 Uniprot
P03372 (ESR1) S167 Uniprot
P03372 (ESR1) S305 Uniprot
P04637 (TP53) S106 Uniprot
P04637 (TP53) S215 Uniprot
P04637 (TP53) S315 Uniprot
P06748 (NPM1) S125 Uniprot
P10275 (AR) S215 Uniprot
P10275 (AR) T282 Uniprot
P10275 (AR) S293 Uniprot
P10275 (AR) S792 Uniprot
P11233 (RALA) S194 Uniprot
P12755 (SKI) S326 Uniprot
P12755 (SKI) S383 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P30305-3 (CDC25B) S312 Uniprot
P30305-2 (CDC25B) S339 Uniprot
P30305 (CDC25B) S353 Uniprot
P38398-1 (BRCA1) S308 Uniprot
P40337 (VHL) S72 Uniprot
P41208 (CETN2) S122 Uniprot
P41208 (CETN2) S170 Uniprot
P49450 (CENPA) S7 Uniprot
P49736 (MCM2) S220 Uniprot
P49841 (GSK3B) S9 Uniprot
P53350 (PLK1) T210 Uniprot
P53667 (LIMK1) S307 Uniprot
P53667 (LIMK1) T508 Uniprot
P53671 (LIMK2) S283 Uniprot
P53671 (LIMK2) T494 Uniprot
P53671 (LIMK2) T505 Uniprot
P61978 (HNRNPK) S379 Uniprot
P68431 (HIST1H3J) S11 Uniprot
Q02224 (CENPE) T422 Uniprot
Q13158 (FADD) S203 Uniprot
Q13526 (PIN1) S16 Uniprot
Q13563 (PKD2) S829 Uniprot
Q14194 (CRMP1) T101 Uniprot
Q14194 (CRMP1) T102 Uniprot
Q14203 (DCTN1) S19 Uniprot
Q14511 (NEDD9) S296 Uniprot
Q14980 (NUMA1) T1804 Uniprot
Q14980 (NUMA1) T1811 Uniprot
Q14980 (NUMA1) T1812 Uniprot
Q14980 (NUMA1) S1887 Uniprot
Q14980 (NUMA1) S1969 Uniprot
Q14980 (NUMA1) S1991 Uniprot
Q14980 (NUMA1) S2047 Uniprot
Q14980 (NUMA1) T2084 Uniprot
Q14980 (NUMA1) S2087 Uniprot
Q15398 (DLGAP5) S627 Uniprot
Q15398 (DLGAP5) S725 Uniprot
Q15398 (DLGAP5) S757 Uniprot
Q15398 (DLGAP5) S830 Uniprot
Q49MG5 (MAP9) S305 Uniprot
Q49MG5 (MAP9) S625 Uniprot
Q8IX03 (WWC1) S539 Uniprot
Q8NHV4 (NEDD1) S405 Uniprot
Q8TEW0 (PARD3) S962 Uniprot
Q8WV24 (PHLDA1) S95 Uniprot
Q92974 (ARHGEF2) S886 Uniprot
Q96GD4 (AURKB) T232 Uniprot
Q9BQG0 (MYBBP1A) S1303 Uniprot
Q9BXS6 (NUSAP1) S240 Uniprot
Q9GZM8 (NDEL1) S251 Uniprot
Q9HC77 (CENPJ) S467 Uniprot
Q9NRM7 (LATS2) S83 Uniprot
Q9NRM7 (LATS2) S380 Uniprot
Q9NS23-2 (RASSF1) T202 Uniprot
Q9NS23-2 (RASSF1) S203 Uniprot
Q9NS23 (RASSF1) T206 Uniprot
Q9NS23 (RASSF1) S207 Uniprot
Q9NWT8 (AURKAIP1) S70 Uniprot
Q9ULW0 (TPX2) S121 Uniprot
Q9ULW0 (TPX2) S125 Uniprot
Q9UNN5 (FAF1) S289 Uniprot
Q9UNN5 (FAF1) S291 Uniprot
Q9UPY8 (MAPRE3) S176 Uniprot
Q9Y6A5 (TACC3) S558 Uniprot

Research Backgrounds


Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1.


Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Thr-288 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-288 during mitosis. Phosphorylation at Ser-342 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-51 at the end of mitosis.

Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome (By similarity). Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. Ubiquitinated by the CUL3-KLHL18 ligase leading to its activation at the centrosome which is required for initiating mitotic entry. Ubiquitination mediated by CUL3-KLHL18 ligase does not lead to its degradation by the proteasome.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cell projection>Neuron projection.
Note: Detected at the neurite hillock in developing neurons (By similarity). Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase (PubMed:9606188, PubMed:17229885, PubMed:21225229). Colocalized with SIRT2 at centrosome (PubMed:22014574). Moves to the midbody during both telophase and cytokinesis (PubMed:17726514). Associates with both the pericentriolar material (PCM) and centrioles (PubMed:22014574). The localization to the spindle poles is regulated by AAAS (PubMed:26246606).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

Subunit Structure:

Interacts with FBXL7 (By similarity). Interacts with CPEB1, JTB, TACC1, TPX2, PPP2CA, as well as with the protein phosphatase type 1 (PP1) isoforms PPP1CA, PPP1CB and PPP1CC. Interacts also with its substrates ARHGEF2, BORA, BRCA1, KIF2A, PARD3, and p53/TP53. Interaction with BORA promotes phosphorylation of PLK1. Interacts with PIFO. Interacts with GADD45A, competing with its oligomerization. Interacts (via C-terminus) with AUNIP (via C-terminus). Identified in a complex with AUNIP and NIN. Interacts with FRY; this interaction facilitates AURKA-mediated PLK1 phosphorylation. Interacts with SIRT2. Interacts with MYCN; interaction is phospho-independent and triggers AURKA activation; AURKA competes with FBXW7 for binding to unphosphorylated MYCN but not for binding to phosphorylated MYCN. Interacts with HNRNPU. Interacts with AAAS. Interacts with KLHL18 and CUL3. Interacts with FOXP1.


Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

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