PHAP1 Antibody | Affinity Biosciences

Product:	PHAP1 Antibody
Catalog:	DF13532
Description:	Rabbit polyclonal antibody to PHAP1
Application:	WB IHC IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep
Mol.Wt.:	28kDa; 29kD(Calculated).
Uniprot:	P39687
RRID:	AB_2846551

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%)

Clonality:

Polyclonal

Specificity:

PHAP1 Antibody detects endogenous levels of total PHAP1.

RRID:

AB_2846551
Cite Format: Affinity Biosciences Cat# DF13532, RRID:AB_2846551.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A; Acidic leucine-rich nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein pp32; AN32A_HUMAN; ANP32A; C15orf1; Cerebellar leucine rich acidic nuclear protein; Hepatopoietin Cn; HPPCn; I1PP2A; Inhibitor 1 of protein phosphatase 2A; inhibitor-1 of protein phosphatase-2A; Lanp; Leucine rich acidic nuclear protein; Leucine-rich acidic nuclear protein; MAPM; Mapmodulin; MGC119787; MGC150373; PHAPI; Potent heat stable protein phosphatase 2A inhibitor I1PP2A; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A; PP32; Putative HLA DR associated protein I; Putative HLA-DR-associated protein I; Putative human HLA class II associated protein I; Putative human HLA class II-associated protein;

Immunogens

Immunogen:

A synthesized peptide derived from human PHAP1, corresponding to a region within C-terminal amino acids.

Uniprot:

P39687(AN32A_HUMAN) >>Visit HPA database.

Gene(ID):

ANP32A(8125)

Expression:

P39687 AN32A_HUMAN:

Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.

Sequence:

P39687 AN32A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEMGRRIHLELRNRTPSDVKELVLDNSRSNEGKLEGLTDEFEELEFLSTINVGLTSIANLPKLNKLKKLELSDNRVSGGLEVLAEKCPNLTHLNLSGNKIKDLSTIEPLKKLENLKSLDLFNCEVTNLNDYRENVFKLLPQLTYLDGYDRDDKEAPDSDAEGYVEGLDDEEEDEDEEEYDEDAQVVEDEEDEDEEEEGEEEDVSGEEEEDEEGYNDGEVDDEEDEEELGEEERGQKRKREPEDEGEDDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Horse

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P39687_AN32A_HUMAN

Function:

Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.

PTMs:

Phosphorylated on serine residues.

The N-terminus is blocked.

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).

Subcellular Location:

Nucleus. Cytoplasm. Endoplasmic reticulum.
Note: Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.

Tissue Specificity:

Family&Domains:

Belongs to the ANP32 family.

References

1). ANP32 Family as Diagnostic, Prognostic, and Therapeutic Biomarker Related to Immune Infiltrates in Hepatocellular Carcinoma. Disease Markers, 2022 (PubMed: 35280441)

Application: WB Species: Mice Sample: HCC tissues and normal liver tissues

Figure 1 Expression and diagnostic value of ANP32 family members in hepatocellular carcinoma (HCC). (a) Differential expression of ANP32 family members between HCC tissues and normal liver tissues in the TCGA + GETx cohort. (b) Differential expression of ANP32 family members between HCC tissues and normal liver tissues in paired samples from the TCGA cohort. (c) Expression of ANP32 family members in normal liver tissues, primary HCC tissues, and metastatic HCC tissues by TNM plot. (d) Expression of ANP32 family members in HCC tissues and normal liver tissues by immunohistochemistry. (e) Expression of ANP32 family members in HCC tissues and normal liver tissues by western blot. (f) Quantification of western blot data. (g)–(i) Diagnostic ROC curves of ANP32A (g), ANP32B (h), and ANP32E (i) in the TCGA + GETx cohort.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PHAP1 Antibody - #DF13532