Histone H1.5 Antibody - #DF13539

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Product: Histone H1.5 Antibody
Catalog: DF13539
Description: Rabbit polyclonal antibody to Histone H1.5
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 22kDa; 23kD(Calculated).
Uniprot: P16401
RRID: AB_2846558

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Histone H1.5 Antibody detects endogenous levels of total Histone H1.5.
RRID:
AB_2846558
Cite Format: Affinity Biosciences Cat# DF13539, RRID:AB_2846558.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

H1; H1 histone family member 5; H1.5; H15 HUMAN; H15_HUMAN; H1B; H1F5; H1s 3; Hist1h1b; Histone 1 H1b; Histone cluster 1 H1b; Histone H1.5; Histone H1a; Histone H1b; Histone H1s 3; MGC126630; MGC126632;

Immunogens

Immunogen:

A synthesized peptide derived from human Histone H1.5, corresponding to a region within N-terminal amino acids.

Uniprot:

P16401(H15_HUMAN) >>Visit HPA database.

Gene(ID):
HIST1H1B(3009)
Expression:
P16401 H15_HUMAN:

Ubiquitous. Expressed in the majority of the cell lines tested and in testis.

Sequence:
MSETAPAETATPAPVEKSPAKKKATKKAAGAGAAKRKATGPPVSELITKAVAASKERNGLSLAALKKALAAGGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGATPKKAKKAAGAKKAVKKTPKKAKKPAAAGVKKVAKSPKKAKAAAKPKKATKSPAKPKAVKPKAAKPKAAKPKAAKPKAAKAKKAAAKKK

Research Backgrounds

Function:

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

PTMs:

H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter (By similarity). Phosphorylated at Thr-11 by GSK3B during mitosis in prometaphase and dephosphorylated in telophase.

Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.

Subcellular Location:

Nucleus. Chromosome.
Note: According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Expressed in the majority of the cell lines tested and in testis.

Family&Domains:

The C-terminal domain is required for high-affinity binding to chromatin.

Belongs to the histone H1/H5 family.

References

1). Downregulation of lncRNA APCDD1L-AS1 due to DNA hypermethylation and loss of VHL protein expression promotes the progression of clear cell renal cell carcinoma. International Journal of Biological Sciences, 2022 (PubMed: 35414787) [IF=8.2]

Application: WB    Species: Mice    Sample: OSRC2 cells

Figure 7 APCDD1L-AS1 overexpression induced histones expression disorders. A The overexpression efficiency of APCDD1L-AS1 in OSRC2 cells. B The TMT results showed that 39 proteins were downregulated and 66 proteins were upregulated in APCDD1L-AS1 overexpressed OSRC2 cells compared with its control cells. C Biological Process GO term enrichment analysis results of the complete 105 statistically significant proteins. D The TMT results of Histone H3.1, Histone H4, Histone H3, Histone H1.3, Histone H1.5, Histone H1.4 and Histone H1.2. E The protein expression of Histone H3.1, Histone H4, Histone H3, Histone H1.3, Histone H1.5, Histone H1.4 and Histone H1.2 in the same cell protein samples. F The protein expression of Histone H3.1, Histone H4, Histone H3, Histone H1.3, Histone H1.5, Histone H1.4 and Histone H1.2 in the in mice tumors.

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