Necdin Antibody - #DF13540
| Product: | Necdin Antibody |
| Catalog: | DF13540 |
| Description: | Rabbit polyclonal antibody to Necdin |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 36kDa; 36kD(Calculated). |
| Uniprot: | Q99608 |
| RRID: | AB_2846559 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13540, RRID:AB_2846559.
Fold/Unfold
HsT16328; NDN; NECD_HUMAN; Necdin; Necdin homolog; Necdin MAGE family member; necdin related protein; PWCR;
Immunogens
Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons.
- Q99608 NECD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEQSKDLSDPNFAAEAPNSEVHSSPGVSEGVPPSATLAEPQSPPLGPTAAPQAAPPPQAPNDEGDPKALQQAAEEGRAHQAPSAAQPGPAPPAPAQLVQKAHELMWYVLVKDQKKMIIWFPDMVKDVIGSYKKWCRSILRRTSLILARVFGLHLRLTSLHTMEFALVKALEPEELDRVALSNRMPMTGLLLMILSLIYVKGRGARESAVWNVLRILGLRPWKKHSTFGDVRKLITEEFVQMNYLKYQRVPYVEPPEYEFFWGSRASREITKMQIMEFLARVFKKDPQAWPSRYREALEEARALREANPTAHYPRSSVSED
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99608 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K116 | Acetylation | Uniprot | |
| K126 | Acetylation | Uniprot | |
| Y132 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot |
Research Backgrounds
Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences (By similarity).
Perikaryon. Nucleus.
Note: Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons.
Binds to the transactivation domains of E2F1 and p53. Binds also SV40 large T antigen and adenovirus E1A. Interacts with nucleobindin 1 and 2 (By similarity).
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