SMARCA1 Antibody - #DF13543

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Product: SMARCA1 Antibody
Catalog: DF13543
Description: Rabbit polyclonal antibody to SMARCA1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 122kDa; 123kD(Calculated).
Uniprot: P28370
RRID: AB_2846562

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
SMARCA1 Antibody detects endogenous levels of total SMARCA1.
RRID:
AB_2846562
Cite Format: Affinity Biosciences Cat# DF13543, RRID:AB_2846562.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent helicase SMARCA1; global transcription activator homologous sequence; ISWI; Nucleosome remodeling factor subunit SNF2L; NURF140; Probable global transcription activator SNF2L1; SMARCA1; SNF2-like 1; SNF2L; SNF2L1; SNF2LB; SNF2LT; sucrose nonfermenting 2-like protein 1; SWI; SWI/SNF related matrix associated actin dependent regulator of chromatin a1; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 1; SWI2;

Immunogens

Immunogen:
Uniprot:

P28370(SMCA1_HUMAN) >>Visit HPA database.

Gene(ID):
SMARCA1(6594)
Sequence:
MEQDTAAVAATVAAADATATIVVIEDEQPGPSTSQEEGAAAAATEATAATEKGEKKKEKNVSSFQLKLAAKAPKSEKEMDPEYEEKMKADRAKRFEFLLKQTELFAHFIQPSAQKSPTSPLNMKLGRPRIKKDEKQSLISAGDYRHRRTEQEEDEELLSESRKTSNVCIRFEVSPSYVKGGPLRDYQIRGLNWLISLYENGVNGILADEMGLGKTLQTIALLGYLKHYRNIPGPHMVLVPKSTLHNWMNEFKRWVPSLRVICFVGDKDARAAFIRDEMMPGEWDVCVTSYEMVIKEKSVFKKFHWRYLVIDEAHRIKNEKSKLSEIVREFKSTNRLLLTGTPLQNNLHELWALLNFLLPDVFNSADDFDSWFDTKNCLGDQKLVERLHAVLKPFLLRRIKTDVEKSLPPKKEIKIYLGLSKMQREWYTKILMKDIDVLNSSGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDEHIVSNSGKMVVLDKLLAKLKEQGSRVLIFSQMTRLLDILEDYCMWRGYEYCRLDGQTPHEEREDKFLEVEFLGQREAIEAFNAPNSSKFIFMLSTRAGGLGINLASADVVILYDSDWNPQVDLQAMDRAHRIGQKKPVRVFRLITDNTVEERIVERAEIKLRLDSIVIQQGRLIDQQSNKLAKEEMLQMIRHGATHVFASKESELTDEDITTILERGEKKTAEMNERLQKMGESSLRNFRMDIEQSLYKFEGEDYREKQKLGMVEWIEPPKRERKANYAVDAYFREALRVSEPKIPKAPRPPKQPNVQDFQFFPPRLFELLEKEILYYRKTIGYKVPRNPDIPNPALAQREEQKKIDGAEPLTPEETEEKEKLLTQGFTNWTKRDFNQFIKANEKYGRDDIDNIAREVEGKSPEEVMEYSAVFWERCNELQDIEKIMAQIERGEARIQRRISIKKALDAKIARYKAPFHQLRIQYGTSKGKNYTEEEDRFLICMLHKMGFDRENVYEELRQCVRNAPQFRFDWFIKSRTAMEFQRRCNTLISLIEKENMEIEERERAEKKKRATKTPMVKFSAFS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28370 As Substrate

Site PTM Type Enzyme
Y83 Phosphorylation
T102 Phosphorylation
S112 Phosphorylation
S116 Phosphorylation
T118 Phosphorylation
S119 Phosphorylation
S137 Phosphorylation
S140 Phosphorylation
Y144 Phosphorylation
S159 Phosphorylation
S161 Phosphorylation
S174 Phosphorylation
K179 Ubiquitination
K322 Ubiquitination
T374 Phosphorylation
Y416 Phosphorylation
S420 Phosphorylation
K421 Ubiquitination
K429 Ubiquitination
K443 Acetylation
K443 Ubiquitination
K639 Ubiquitination
S644 Phosphorylation
S657 Phosphorylation
K659 Ubiquitination
K662 Ubiquitination
K680 Ubiquitination
T685 Phosphorylation
S713 Phosphorylation
S714 Phosphorylation
S725 Phosphorylation
K754 Sumoylation
K754 Ubiquitination
Y757 Phosphorylation
Y762 Phosphorylation
S770 Phosphorylation
K814 Ubiquitination
K851 Acetylation
K851 Ubiquitination
K870 Ubiquitination
K874 Ubiquitination
K944 Sumoylation
K944 Ubiquitination
Y954 Phosphorylation
K960 Ubiquitination
Y962 Phosphorylation
Y985 Phosphorylation
K1038 Acetylation
K1039 Acetylation

Research Backgrounds

Function:

Energy-transducing component of NURF (nucleosome-remodeling factor) and CERF (CECR2-containing-remodeling factor) complexes. Both complexes facilitate the perturbation of chromatin structure in an ATP-dependent manner. Potentiates neurite outgrowth. May be involved in brain development by regulating En-1 and En-2 expression. May be involved in the development of luteal cells.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Part of the nucleosome-remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Part of the CECR2-containing-remodeling factor (CERF) complex which contains CECR2 and SMARCA1. Interacts with PRLR. Interacts with ERCC6.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.

Restrictive clause

 

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