MAPRE1 Antibody - #DF13549
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13549, RRID:AB_2846568.
Fold/Unfold
5530600P05Rik; Adenomatous polyposis coli binding protein EB 1; Adenomatous polyposis coli binding protein EB1; AI462499; AI504412; APC binding protein EB 1; APC binding protein EB1; APC-binding protein EB1; AW260097; BIM1p; D2Ertd459e; EB 1; EB1; End binding protein 1; End-binding protein 1; fa01e12; fc23e11; fi33c06; MAPRE 1; MAPRE1; Mapre3; MARE1_HUMAN; MGC117374; MGC129946; MGC52508; Microtubule associated protein RP/EB family member 1; Microtubule-associated protein RP/EB family member 1; wu:fa01e12; wu:fc23e11; wu:fi33c06; zgc:55428; zgc:77807; zgc:85755;
Immunogens
- Q15691 MARE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVNVYSTSVTSDNLSRHDMLAWINESLQLNLTKIEQLCSGAAYCQFMDMLFPGSIALKKVKFQAKLEHEYIQNFKILQAGFKRMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGKDYDPVAARQGQETAVAPSLVAPALNKPKKPLTSSSAAPQRPISTQRTAAAPKAGPGVVRKNPGVGNGDDEAAELMQQVNVLKLTVEDLEKERDFYFGKLRNIELICQENEGENDPVLQRIVDILYATDEGFVIPDEGGPQEEQEEY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15691 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| Y6 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| K60 | Acetylation | Uniprot | |
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| Y71 | Phosphorylation | Uniprot | |
| K76 | Acetylation | Uniprot | |
| K76 | Methylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K83 | Acetylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K89 | Acetylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| Y119 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K151 | Methylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| T206 | Phosphorylation | Uniprot | |
| K212 | Sumoylation | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| K220 | Acetylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| Y268 | Phosphorylation | Uniprot |
Research Backgrounds
Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. Acts as a regulator of autophagosome transport via interaction with CAMSAP2. May play a role in cell migration (By similarity).
Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Golgi apparatus.
Note: Associated with the microtubule growing distal tips (PubMed:28814570). Recruitment to the Golgi apparatus requires the presence of PDE4DIP isoform 13/MMG8/SMYLE (PubMed:25217626).
Ubiquitously expressed.
Homodimer. Heterodimer with MAPRE3. Interacts with DCTN1, DCTN2, TERF1 and dynein intermediate chain. Interaction with DIAPH1 and DIAPH2 (By similarity). Interacts with APC (via C-terminal domain), CLASP2, DST, KIF2C and STIM1; probably required for their targeting to the growing microtubule plus ends. Interacts with MTUS2; interaction is direct and probably targets MTUS2 to microtubules. Interacts with APC2. Interacts with CLASP1. Interacts with CDK5RAP2. Interacts with MACF1 (By similarity). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with KCNAB2 (By similarity). Interacts (via C-terminus) with CLIP1. Interacts with SLAIN2 and SLAIN1. Interacts with KIF18B; this interaction is required for efficient accumulation of KIF18B at microtubule plus ends. Interacts with MISP. Interacts with KNSTRN. Interacts with NCKAP5L. Interacts with CAMSAP2. Interacts with PDE4DIP isoform 13/MMG8/SMYLE; this interaction is required for its recruitment to the Golgi apparatus. Forms a pericentrosomal complex with AKAP9, CDK5RAP2 and PDE4DIP isoform 13/MMG8/SMYLE; within this complex, MAPRE1 binding to CDK5RAP2 may be mediated by PDE4DIP. Interacts with AKNA (By similarity). Interacts with GAS2L1, GAS2L2, and GAS2L3.
Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
Belongs to the MAPRE family.
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