SERBP1 Antibody | Affinity Biosciences

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SERBP1 Antibody - Western blot analysis of extracts from RT4, using SERBP1 Antibody.

SERBP1 Antibody - Figure 1 SERPINE1 is upregulated in hemin-treated HT22 cells.

Product:	SERBP1 Antibody
Catalog:	DF13553
Description:	Rabbit polyclonal antibody to SERBP1
Application:	WB
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	44kDa; 45kD(Calculated).
Uniprot:	Q8NC51
RRID:	AB_2846572

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

SERBP1 Antibody detects endogenous levels of total SERBP1.

RRID:

AB_2846572
Cite Format: Affinity Biosciences Cat# DF13553, RRID:AB_2846572.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CGI 55; CHD3IP; Chromodomain helicase DNA binding protein 3 interacting protein; DKFZp564M2423; FLJ90489; HABP4L; PAI 1 mRNA binding protein; PAI RBP1; PAI-RBP1; PAI1 RNA-binding protein 1; PAIRB_HUMAN; PAIRBP1; Plasminogen activator inhibitor 1 RNA binding protein; Plasminogen activator inhibitor 1 RNA-binding protein; Serbp1; SERPINE1 mRNA binding protein 1; SERPINE1 mRNA-binding protein 1;

Immunogens

Immunogen:

A synthesized peptide derived from human SERBP1, corresponding to a region within the internal amino acids.

Uniprot:

Q8NC51(PAIRB_HUMAN) >>Visit HPA database.

Gene(ID):

SERBP1(26135)

Expression:

Q8NC51 PAIRB_HUMAN:

Expressed at high level in the heart, skeletal muscle and kidney, and at low levels in placenta, liver and brain.

Sequence:

Q8NC51 PAIRB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPGHLQEGFGCVVTNRFDQLFDDESDPFEVLKAAENKKKEAGGGGVGGPGAKSAAQAAAQTNSNAAGKQLRKESQKDRKNPLPPSVGVVDKKEETQPPVALKKEGIRRVGRRPDQQLQGEGKIIDRRPERRPPRERRFEKPLEEKGEGGEFSVDRPIIDRPIRGRGGLGRGRGGRGRGMGRGDGFDSRGKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNYSDLDQSNVTEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGADGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINFGDLGRPGRGGRGGRGGRGRGGRPNRGSRTDKSSASAPDVDDPEAFPALA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Bovine

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q8NC51_PAIRB_HUMAN

Function:

May play a role in the regulation of mRNA stability. Binds to the 3'-most 134 nt of the SERPINE1/PAI1 mRNA, a region which confers cyclic nucleotide regulation of message decay. Seems to play a role in PML-nuclear bodies formation.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Perinuclear region.

Tissue Specificity:

Expressed at high level in the heart, skeletal muscle and kidney, and at low levels in placenta, liver and brain.

References

1). Germacrone protects renal tubular cells against ferroptotic death and ROS release by re-activating mitophagy in diabetic nephropathy. Free radical research, 2023 (PubMed: 37897414) [IF=3.6]

2). HERC5/ISG15 Enhances Glioblastoma Stemness and Tumor Progression by mediating SERBP1protein stability. Neuromolecular medicine, 2025 (PubMed: 39776018) [IF=3.5]

3). Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin‑induced in vitro ototoxicity in HEI‑OC1 auditory cells using gene expression profiling. Molecular Medicine Reports, 2020 (PubMed: 33174043) [IF=3.4]

Application: WB Species: Mice Sample: HEI-OC1 cells

Figure 5. FPKM values and expression of several DEGs in the three groups. (A-D) FPKM values of several random selected up- or downregulated DEGs with typical expression profiling in response to treatment with cisplatin alone or combined with Tet. (E) Expression of several protein products of DEGs. *P<0.05, vs. control. #P<0.05 vs. Cisplatin. The differences were analyzed by one-way ANOVA followed by Tukey's post hoc test. DEG, differentially expressed gene; FKPM, fragments per kilobase of exon model per million reads mapped.

4). TGF-β1-Mediated Activation of SERPINE1 is Involved in Hemin-Induced Apoptotic and Inflammatory Injury in HT22 Cells. Neuropsychiatric Disease and Treatment, 2021 (PubMed: 33603380) [IF=2.5]

Application: WB Species: Mice Sample: HT22 cells

Figure 1 SERPINE1 is upregulated in hemin-treated HT22 cells. (A) HT22 cells were treated with different doses (0, 10, 20, 40, 60, 80, 100, and 120 μM) of hemin for 24 h and then subjected to MTT assay. (B) HT22 cells were treated with 100 μM of hemin for 24 h and then assayed for SERPINE1 expression using qRT-PCR assay. (C) Representative image of Western blot analysis and quantification of SERPINE1 expression in HT22 cells stimulated with hemin. *p < 0.05, **p <0.01, and ***p <0.001.

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SERBP1 Antibody - #DF13553