Nanos3 Antibody - #DF13559
| Product: | Nanos3 Antibody |
| Catalog: | DF13559 |
| Description: | Rabbit polyclonal antibody to Nanos3 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Sheep |
| Mol.Wt.: | 18kDa; 19kD(Calculated). |
| Uniprot: | P60323 |
| RRID: | AB_2846578 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13559, RRID:AB_2846578.
Fold/Unfold
NANO3_HUMAN; Nanos homolog 3; NANOS3; NOS-3; Nos3;
Immunogens
Ovary, testis and brain (at protein level). In the ovaries, expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids.
- P60323 NANO3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGTFDLWTDYLGLAHLVRALSGKEGPETRLSPQPEPEPMLEPDQKRSLESSPAPERLCSFCKHNGESRAIYQSHVLKDEAGRVLCPILRDYVCPQCGATRERAHTRRFCPLTGQGYTSVYSHTTRNSAGKKLVRPDKAKTQDTGHRRGGGGGAGFRGAGKSEPSPSCSPSMST
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P60323 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S47 | Phosphorylation | Uniprot | |
| R146 | Methylation | Uniprot | |
| R147 | Methylation | Uniprot | |
| R156 | Methylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot |
Research Backgrounds
Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.
Nucleus. Cytoplasm. Cytoplasm>Stress granule. Cytoplasm>P-body.
Note: Co-localizes with PUM2, EIF2S1 and TIAL1 in the stress granules. Co-localizes with DCP1A in the P-body.
Ovary, testis and brain (at protein level). In the ovaries, expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids.
Binds mRNA from germ cells. Interacts with PUM2 (By similarity).
The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
Belongs to the nanos family.
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