SLFN14 Antibody | Affinity Biosciences

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SLFN14 Antibody - Western blot analysis of extracts from Human kidney, using SLFN14 Antibody.

SLFN14 Antibody - Figure 3 T853fs leads to low expression level of SLFN14 (A and B) HEK293T and Meg-01 cells were transfected with SLFN14-WT or SLFN14-T853fs overexpression plasmids for 48 h.

Product:	SLFN14 Antibody
Catalog:	DF13579
Description:	Rabbit polyclonal antibody to SLFN14
Application:	WB
Cited expt.:	WB
Reactivity:	Human
Prediction:	Horse, Rabbit
Mol.Wt.:	103kDa; 104kD(Calculated).
Uniprot:	P0C7P3
RRID:	AB_2846598

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Horse(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

SLFN14 Antibody detects endogenous levels of total SLFN14.

RRID:

AB_2846598
Cite Format: Affinity Biosciences Cat# DF13579, RRID:AB_2846598.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Schlafen family member 14; SLFN14; SLN14_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human SLFN14, corresponding to a region within C-terminal amino acids.

Uniprot:

P0C7P3(SLN14_HUMAN) >>Visit HPA database.

Gene(ID):

SLFN14(342618)

Expression:

P0C7P3 SLN14_HUMAN:

Expressed in megakaryocytes and platelets (at protein level) (PubMed:26280575). Weakly expressed in melanocytes and malignant melanoma cells (PubMed:20956525).

Sequence:

P0C7P3 SLN14_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MESLKTDTEMPYPEVIVDVGRVIFGEENRKKMTNSCLKRSENSRIIRAICALLNSGGGVIKAEIDDKTYSYQCHGLGQDLETSFQKLLPSGSQKYLDYMQQGHNLLIFVKSWSPDVFSLPLRICSLRSNLYRRDVTSAINLSASSALELLREKGFRAQRGRPRVKKLHPQQVLNRCIQEEEDMRILASEFFKKDKLMYKEKLNFTESTHVEFKRFTTKKVIPRIKEMLPHYVSAFANTQGGYVLIGVDDKSKEVVGCKWEKVNPDLLKKEIENCIEKLPTFHFCCEKPKVNFTTKILNVYQKDVLDGYVCVIQVEPFCCVVFAEAPDSWIMKDNSVTRLTAEQWVVMMLDTQSAPPSLVTDYNSCLISSASSARKSPGYPIKVHKFKEALQRHLFPVTQEEVQFKPESLCKKLFSDHKELEGLMKTLIHPCSQGIVIFSRSWAGDVGFRKEQNVLCDALLIAVNSPVVLYTILIDPNWPGGLEYARNTAHQLKQKLQTVGGYTGKVCIIPRLIHLSSTQSRPGEIPLRYPRSYRLADEEEMEDLLQALVVVSLSSRSLLSDQMGCEFFNLLIMEQSQLLSESLQKTRELFIYCFPGVRKTALAIKIMEKIKDLFHCKPKEILYVCESDSLKDFVTQQTTCQAVTRKTFMQGEFLKIKHIVMDETENFCSKYGNWYMKAKNITHPKAKGTGSENLHHGILWLFLDPFQIHHADVNGLPPPSAQFPRKTITSGIHCALEIAKVMKEEMKRIKENPPSNMSPDTLALFSETAYEEATCAQALPGVCETKTNLTTEQIANYVARKCHSLFQCGYLPKDIAILCRRGEDRGRYRLALLKAMELIETHRPSEVVFSPATGVWGSHIVLDSIQQFSGLERTVVFGLSPECDQSEEFHKLCFASRAIKHLYLLYEKRAAY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

100

Pig

Dog

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P0C7P3_SLN14_HUMAN

Function:

Shows no ribosome-associated and endoribonuclease activities.

Displays polysome-associated endoribonuclease activity towards mRNAs and rRNAs. May play a role in RNA surveillance pathways by recognizing stalled ribosomes and triggering endonucleolytic cleavage of aberrant mRNAs (Probable). Cleaves different types of rRNAs and mRNAs in a magnesium- and manganese-dependent and ATP-independent manner (By similarity). Involved in correct maturation of megakaryocytes and especially important for proplatelet extension.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in megakaryocytes and platelets (at protein level). Weakly expressed in melanocytes and malignant melanoma cells.

Family&Domains:

Belongs to the Schlafen family. Subgroup III subfamily.

References

1). Novel mutation SLFN14 T853fs associated with inherited macrothrombocytopenia. Molecular therapy. Nucleic acids, 2025 (PubMed: 40510593) [IF=6.5]

Application: WB Species: human Sample:

Figure 3 T853fs leads to low expression level of SLFN14 (A and B) HEK293T and Meg-01 cells were transfected with SLFN14-WT or SLFN14-T853fs overexpression plasmids for 48 h. The expressions of exogenous SLFN14 were determined by (A) real time qPCR and (B) western blot. Both representative figures and quantification data for western blot are shown. GAPDH was used as an internal reference. (C) Western blot analysis of SLFN14 protein expression in platelet lysates of the proband and two healthy subjects (control 1 and control 2). Both representative figures and quantification data are shown. (D) rRNA degradation in SLFN14-WT and SLFN14-T853fs) overexpressing HEK293T cells was assessed by denaturing agarose/formaldehyde gel electrophoresis (n = 3 independent experiments). (E) Cell proliferation of MEG-01 cells that were transfected by empty vector (EV), SLFN14-WT, or SLFN14-T853fs overexpression plasmids was determined by Cell Counting Kit-8 (CCK-8) assay. (F) Immunofluorescence staining against SLFN14 (red) of MEG-01 cells transfected with SLFN14-WT or SLFN14-T853fs overexpression plasmids for 48 h. Nuclei were counterstained with DAPI (blue). Merged images indicate the overlay of SLFN14 and nuclei signals. Images were captured using a 60× objective. Scale bars: 20 μm. Data are presented as mean ± standard deviation (SD). Comparisons between two groups were conducted using unpaired two-tailed Student’s t test.

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SLFN14 Antibody - #DF13579