SLFN14 Antibody | Affinity Biosciences

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SLFN14 Antibody - Western blot analysis of extracts from Human kidney, using SLFN14 Antibody.

Product:	SLFN14 Antibody
Catalog:	DF13579
Description:	Rabbit polyclonal antibody to SLFN14
Application:	WB
Reactivity:	Human
Prediction:	Horse, Rabbit
Mol.Wt.:	103kDa; 104kD(Calculated).
Uniprot:	P0C7P3
RRID:	AB_2846598

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Horse(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

SLFN14 Antibody detects endogenous levels of total SLFN14.

RRID:

AB_2846598
Cite Format: Affinity Biosciences Cat# DF13579, RRID:AB_2846598.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Schlafen family member 14; SLFN14; SLN14_HUMAN;

Immunogens

Immunogen:

Uniprot:

P0C7P3(SLN14_HUMAN) >>Visit HPA database.

Gene(ID):

SLFN14(342618)

Expression:

P0C7P3 SLN14_HUMAN:

Expressed in megakaryocytes and platelets (at protein level) (PubMed:26280575). Weakly expressed in melanocytes and malignant melanoma cells (PubMed:20956525).

Sequence:

P0C7P3 SLN14_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MESLKTDTEMPYPEVIVDVGRVIFGEENRKKMTNSCLKRSENSRIIRAICALLNSGGGVIKAEIDDKTYSYQCHGLGQDLETSFQKLLPSGSQKYLDYMQQGHNLLIFVKSWSPDVFSLPLRICSLRSNLYRRDVTSAINLSASSALELLREKGFRAQRGRPRVKKLHPQQVLNRCIQEEEDMRILASEFFKKDKLMYKEKLNFTESTHVEFKRFTTKKVIPRIKEMLPHYVSAFANTQGGYVLIGVDDKSKEVVGCKWEKVNPDLLKKEIENCIEKLPTFHFCCEKPKVNFTTKILNVYQKDVLDGYVCVIQVEPFCCVVFAEAPDSWIMKDNSVTRLTAEQWVVMMLDTQSAPPSLVTDYNSCLISSASSARKSPGYPIKVHKFKEALQRHLFPVTQEEVQFKPESLCKKLFSDHKELEGLMKTLIHPCSQGIVIFSRSWAGDVGFRKEQNVLCDALLIAVNSPVVLYTILIDPNWPGGLEYARNTAHQLKQKLQTVGGYTGKVCIIPRLIHLSSTQSRPGEIPLRYPRSYRLADEEEMEDLLQALVVVSLSSRSLLSDQMGCEFFNLLIMEQSQLLSESLQKTRELFIYCFPGVRKTALAIKIMEKIKDLFHCKPKEILYVCESDSLKDFVTQQTTCQAVTRKTFMQGEFLKIKHIVMDETENFCSKYGNWYMKAKNITHPKAKGTGSENLHHGILWLFLDPFQIHHADVNGLPPPSAQFPRKTITSGIHCALEIAKVMKEEMKRIKENPPSNMSPDTLALFSETAYEEATCAQALPGVCETKTNLTTEQIANYVARKCHSLFQCGYLPKDIAILCRRGEDRGRYRLALLKAMELIETHRPSEVVFSPATGVWGSHIVLDSIQQFSGLERTVVFGLSPECDQSEEFHKLCFASRAIKHLYLLYEKRAAY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

100

Pig

Dog

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P0C7P3 As Substrate

P0C7P3

Site	PTM Type	Source
Y198	Phosphorylation	Uniprot
S233	Phosphorylation	Uniprot
T238	Phosphorylation	Uniprot
Y242	Phosphorylation	Uniprot
Y300	Phosphorylation	Uniprot
S439	Phosphorylation	Uniprot
K495	Ubiquitination	Uniprot
T600	Phosphorylation	Uniprot
S627	Phosphorylation	Uniprot
S629	Phosphorylation	Uniprot
T647	Phosphorylation	Uniprot

Research Backgrounds

P0C7P3_SLN14_HUMAN

Function:

Shows no ribosome-associated and endoribonuclease activities.

Displays polysome-associated endoribonuclease activity towards mRNAs and rRNAs. May play a role in RNA surveillance pathways by recognizing stalled ribosomes and triggering endonucleolytic cleavage of aberrant mRNAs (Probable). Cleaves different types of rRNAs and mRNAs in a magnesium- and manganese-dependent and ATP-independent manner (By similarity). Involved in correct maturation of megakaryocytes and especially important for proplatelet extension.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in megakaryocytes and platelets (at protein level). Weakly expressed in melanocytes and malignant melanoma cells.

Subunit Structure:

Associates with ribosomes in an ATP-independent manner.

Family&Domains:

Belongs to the Schlafen family. Subgroup III subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SLFN14 Antibody - #DF13579