C9orf142 Antibody - #DF13585
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13585, RRID:AB_2846604.
Fold/Unfold
C9orf142; Chromosome 9 open reading frame 142; CI142_HUMAN; PAXX; Uncharacterized protein C9orf142; XLS;
Immunogens
- Q9BUH6 PAXX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAALKARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPRLRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPDPQRGGPGPGVRRRCPGESLINPGFKSKKPAGGVDFDET
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BUH6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| R169 | Methylation | Uniprot | |
| C180 | S-Nitrosylation | Uniprot | |
| K191 | Acetylation | Uniprot | |
| S192 | Phosphorylation | Uniprot |
Research Backgrounds
Involved in non-homologous end joining (NHEJ), a major pathway to repair double-strand breaks in DNA. May act as a scaffold required to stabilize the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites and promote the assembly and/or stability of the NHEJ machinery.
Nucleus.
Note: Predominantly localizes to the nucleus. Accumulates at sites of DNA damage generated by laser microirradiation.
Homodimer. Interacts with the DNA-bound XRCC5/Ku80 and XRCC6/Ku70 heterodimer (Ku complex); the interaction is direct. Associates with the non-homologous end joining (NHEJ) complex which is at least composed of the core proteins XRCC5/Ku80, XRCC6/Ku70, PRKDC/DNA-PKcs, LIG4 and XRCC4. Additional components of the NHEJ complex include NHEJ1/XLF and C9orf142/PAXX.
The N-terminus (residues 1-113) forms a head domain that is structurally related to those of XRCC4, XLF/NHEJ1, and SASS6.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.