Product: Survivin Antibody
Catalog: AF6017
Description: Rabbit polyclonal antibody to Survivin
Application: WB IHC IF/ICC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 20kDa; 16kD(Calculated).
Uniprot: O15392
RRID: AB_2834951

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
Survivin Antibody detects endogenous levels of total Survivin.
RRID:
AB_2834951
Cite Format: Affinity Biosciences Cat# AF6017, RRID:AB_2834951.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

API4; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; Apoptosis inhibitor4; Baculoviral IAP repeat containing 5; Baculoviral IAP repeat containing protein 5; Baculoviral IAP repeat-containing protein 5; BIRC 5; BIRC5; BIRC5_HUMAN; EPR 1; IAP4; Survivin variant 3 alpha; SVV; TIAP;

Immunogens

Immunogen:

A synthesized peptide derived from human Survivin, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
O15392 BIRC5_HUMAN:

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain (PubMed:10626797). Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas (PubMed:14741722, PubMed:16329164). Also expressed in various renal cell carcinoma cell lines (PubMed:10626797). Expressed in cochlea including the organ of Corti, the lateral wall, the interdental cells of the Limbus as well as in Schwann cells and cells of the cochlear nerve and the spiral ganglions (at protein level). Not expressed in cells of the inner and outer sulcus or the Reissner's membrane (at protein level) (PubMed:21364656, PubMed:20627126).

Description:
survivin is an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia.
Sequence:
MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Essential for the maintenance of mitochondrial integrity and function. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

PTMs:

Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting.

In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes. Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities. Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity. Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis.

Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.

Subcellular Location:

Cytoplasm. Nucleus. Chromosome. Chromosome>Centromere. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Centromere>Kinetochore. Midbody.
Note: Localizes at the centromeres from prophase to metaphase, at the spindle midzone during anaphase and a the midbody during telophase and cytokinesis. Accumulates in the nucleus upon treatment with leptomycin B (LMB), a XPO1/CRM1 nuclear export inhibitor (By similarity). Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody (PubMed:11084331). Colocalizes with AURKB at mitotic chromosomes (PubMed:14610074). Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export (PubMed:20826784).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. Expressed in cochlea including the organ of Corti, the lateral wall, the interdental cells of the Limbus as well as in Schwann cells and cells of the cochlear nerve and the spiral ganglions (at protein level). Not expressed in cells of the inner and outer sulcus or the Reissner's membrane (at protein level).

Family&Domains:

The BIR repeat is necessary and sufficient for LAMTOR5 binding.

Belongs to the IAP family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

References

1). Gut dysbiosis promotes prostate cancer progression and docetaxel resistance via activating NF-κB-IL6-STAT3 axis. Microbiome, 2022 (PubMed: 35710492) [IF=13.8]

Application: WB    Species: Mouse    Sample: tumor tissue

Fig. 5 The IL6-STAT3 pathway promoted prostate cancer docetaxel chemoresistance. A Western blot of relative proteins in RM-1 and DU-145 cultured with different conditions. B, C Cell viability and TUNEL assay (scale bar, 100 μm) were conducted on RM-1 and DU-145 under condition as described. D Flowchart of the NC, Docetaxel, Abx+Docetaxel, and Abx+Docetaxel+Stattic groups for in vivo study. Relevant tumor images and comparison of volume and weight for tumors in four groups (n = 5). E Immunohistochemistry of tumor tissues for p-STAT3-, Bcl-2-, and survivin-positive cell in four groups (scale bar, 50 μm). Statistical significance was assessed by LSD in one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001

Application: IHC    Species: Mouse    Sample: tumor tissue

Fig. 5 The IL6-STAT3 pathway promoted prostate cancer docetaxel chemoresistance. A Western blot of relative proteins in RM-1 and DU-145 cultured with different conditions. B, C Cell viability and TUNEL assay (scale bar, 100 μm) were conducted on RM-1 and DU-145 under condition as described. D Flowchart of the NC, Docetaxel, Abx+Docetaxel, and Abx+Docetaxel+Stattic groups for in vivo study. Relevant tumor images and comparison of volume and weight for tumors in four groups (n = 5). E Immunohistochemistry of tumor tissues for p-STAT3-, Bcl-2-, and survivin-positive cell in four groups (scale bar, 50 μm). Statistical significance was assessed by LSD in one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001

2). Increased autophagy in EOC re-ascites cells can inhibit cell death and promote drug resistance. Cell Death & Disease, 2018 (PubMed: 29549251) [IF=8.1]

3). Dehydroepiandrosterone attenuates pulmonary artery and right ventricular remodeling in a rat model of pulmonary hypertension due to left heart failure. LIFE SCIENCES, 2019 (PubMed: 30605649) [IF=5.2]

Application: WB    Species: rat    Sample: lung

Fig. 6. |DHEA suppressed STAT3/NFAT signal pathway in lung. A, PY750-STAT3 and STAT3; B, NFATc2; C, Pim-1; D, Survivin. The top shows representative immunoblots, and the bottom shows the densitometric assessment. The values are means ± SE, n = 5 rats per group; *P < 0.05 versus the sham group, #P < 0.05 versus the PH-LHF group.

4). Anticancer Effect of Puerarin on Ovarian Cancer Progression Contributes to the Tumor Suppressor Gene Expression and Gut Microbiota Modulation. Journal of Immunology Research, 2022 (PubMed: 35935578) [IF=3.5]

5). Gigantol Attenuates the Metastasis of Human Bladder Cancer Cells, Possibly Through Wnt/EMT Signaling. OncoTargets and Therapy, 2020 (PubMed: 33177841) [IF=2.7]

Application: WB    Species: Human    Sample: bladder cancer cells

Figure 3 Cell invasion assays and expression analysis of Wnt/EMT related genes in cells treated with gigantol. (A) Cell invasion was measured by transwell assay in bladder cancer cells treated with increasing concentrations of gigantol (magnification ×100). (B) qRT-PCR analysis of the Wnt target genes and EMT markers in bladder cancer cells treated with gigantol. (C) Western blot analysis of Wnt/EMT markers in bladder cancer cells treated with indicated concentrations of gigantol. Data in (B) were shown as means ± SD (n = 3), the statistically significant differences were considered at *p<0.05, **p<0.01, ***p<0.001.

6). Downregulation of NEAT1 Suppresses Cell Proliferation, Migration, and Invasion in NSCLC Via Sponging miR-153-3p. Cancer biotherapy & radiopharmaceuticals, 2020 (PubMed: 32380843) [IF=2.4]

Application: WB    Species: Human    Sample: A549 and H460 cell

FIG. 6. The Wnt/b-catenin signaling pathway protein expression level in A549 and H460 cell lines detected by Western blot. *p < 0.05, compared with si-NC group, # p < 0.05, in contrast to si-NEAT1+NC inhibitor group.

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