MIS12 Antibody - #DF13646

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Product: MIS12 Antibody
Catalog: DF13646
Description: Rabbit polyclonal antibody to MIS12
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine
Mol.Wt.: 24kDa; 24kD(Calculated).
Uniprot: Q9H081
RRID: AB_2846665

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%)
Clonality:
Polyclonal
Specificity:
MIS12 Antibody detects endogenous levels of total MIS12.
RRID:
AB_2846665
Cite Format: Affinity Biosciences Cat# DF13646, RRID:AB_2846665.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hMIS12; homolog of yeast Mis12; KNTC2AP; MIND kinetochore complex component, homolog (yeast); MIS12 homolog (yeast); MIS12 homolog; MIS12 MIND kinetochore complex component homolog (yeast); MTW 1; MTW1; Protein MIS12 homolog;

Immunogens

Immunogen:
Uniprot:

Q9H081(MIS12_HUMAN) >>Visit HPA database.

Gene(ID):
MIS12(79003)
Sequence:
MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
83
Horse
75
Chicken
71
Sheep
67
Xenopus
57
Pig
0
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H081 As Substrate

Site PTM Type Enzyme
S56 Phosphorylation
K119 Ubiquitination
K129 Ubiquitination
K135 Ubiquitination
K146 Ubiquitination
K151 Ubiquitination

Research Backgrounds

Function:

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Essential for proper kinetochore microtubule attachments.

Subcellular Location:

Chromosome>Centromere>Kinetochore.
Note: Associated with the kinetochore.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1. Also interacts with KNL1, CBX3, CBX5, NDC80 and ZWINT.

Family&Domains:

Belongs to the mis12 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.