RECQL5 Antibody - #DF13664
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13664, RRID:AB_2846683.
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ATP-dependent DNA helicase Q5; DNA helicase; DNA helicase, RecQ like type 5; DNA helicase, RECQ-like, type 5; EC 3.6.1.-; FLJ90603; RecQ helicase protein-like 5 beta; RecQ protein-like 5; RecQ protein-like 5, isoform CRA_b; RecQ-like type 5; RecQ5; RECQ5_HUMAN; Recq5b; RECQL5; Recql5b;
Immunogens
- O94762 RECQ5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSHHTTFPFDPERRVRSTLKKVFGFDSFKTPLQESATMAVVKGNKDVFVCMPTGAGKSLCYQLPALLAKGITIVVSPLIALIQDQVDHLLTLKVRVSSLNSKLSAQERKELLADLEREKPQTKILYITPEMAASSSFQPTLNSLVSRHLLSYLVVDEAHCVSQWGHDFRPDYLRLGALRSRLGHAPCVALTATATPQVQEDVFAALHLKKPVAIFKTPCFRANLFYDVQFKELISDPYGNLKDFCLKALGQEADKGLSGCGIVYCRTREACEQLAIELSCRGVNAKAYHAGLKASERTLVQNDWMEEKVPVIVATISFGMGVDKANVRFVAHWNIAKSMAGYYQESGRAGRDGKPSWCRLYYSRNDRDQVSFLIRKEVAKLQEKRGNKASDKATIMAFDALVTFCEELGCRHAAIAKYFGDALPACAKGCDHCQNPTAVRRRLEALERSSSWSKTCIGPSQGNGFDPELYEGGRKGYGDFSRYDEGSGGSGDEGRDEAHKREWNLFYQKQMQLRKGKDPKIEEFVPPDENCPLKEASSRRIPRLTVKAREHCLRLLEEALSSNRQSTRTADEADLRAKAVELEHETFRNAKVANLYKASVLKKVADIHRASKDGQPYDMGGSAKSCSAQAEPPEPNEYDIPPASHVYSLKPKRVGAGFPKGSCPFQTATELMETTRIREQAPQPERGGEHEPPSRPCGLLDEDGSEPLPGPRGEVPGGSAHYGGPSPEKKAKSSSGGSSLAKGRASKKQQLLATAAHKDSQSIARFFCRRVESPALLASAPEAEGACPSCEGVQGPPMAPEKYTGEEDGAGGHSPAPPQTEECLRERPSTCPPRDQGTPEVQPTPAKDTWKGKRPRSQQENPESQPQKRPRPSAKPSVVAEVKGSVSASEQGTLNPTAQDPFQLSAPGVSLKEAANVVVKCLTPFYKEGKFASKELFKGFARHLSHLLTQKTSPGRSVKEEAQNLIRHFFHGRARCESEADWHGLCGPQR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O94762 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K22 | Ubiquitination | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| T31 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| K232 | Methylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| T456 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| Y478 | Phosphorylation | Uniprot | |
| Y484 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| S491 | Phosphorylation | Uniprot | |
| Y508 | Phosphorylation | Uniprot | |
| T568 | Phosphorylation | Uniprot | |
| T570 | Phosphorylation | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| K592 | Ubiquitination | Uniprot | |
| T676 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| R745 | Methylation | Uniprot | |
| K749 | Ubiquitination | Uniprot | |
| K759 | Ubiquitination | Uniprot | |
| S774 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| T839 | Phosphorylation | Uniprot | |
| T845 | Phosphorylation | Uniprot | |
| S858 | Phosphorylation | Uniprot | |
| C977 | S-Nitrosylation | Uniprot | |
| C987 | S-Nitrosylation | Uniprot |
Research Backgrounds
Isoform beta is a DNA helicase that plays an important role in DNA replication, transcription and repair. Inhibits elongation of stalled transcripts at DNA damage sites by binding to the RNA polymerase II subunit POLR2A and blocking the TCEA1 binding site. Required for mitotic chromosome separation after cross-over events and cell cycle progress. Required for efficient DNA repair, including repair of inter-strand cross-links. Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination.
Nucleus>Nucleoplasm.
Note: Recruited to sites of DNA damage, such as single-strand breaks and inter-strand cross-links, and at stalled replication forks.
Cytoplasm.
Cytoplasm.
Ubiquitous.
Monomer. Interacts with TOP2A, TOP3A and TOP3B. Isoform beta interacts with RNA polymerase II subunit POLR2A. Identified in a complex with the RNA polymerase II core bound to DNA. Isoform beta interacts with RAD51.
Belongs to the helicase family. RecQ subfamily.
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