Zfp281 Antibody - #DF13689

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Product: Zfp281 Antibody
Catalog: DF13689
Description: Rabbit polyclonal antibody to Zfp281
Application: WB IHC
Reactivity: Human, Rat
Prediction: Horse
Mol.Wt.: 96kDa; 97kD(Calculated).
Uniprot: Q9Y2X9
RRID: AB_2846708

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Horse(100%)
Clonality:
Polyclonal
Specificity:
Zfp281 Antibody detects endogenous levels of total Zfp281.
RRID:
AB_2846708
Cite Format: Affinity Biosciences Cat# DF13689, RRID:AB_2846708.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GC box binding zinc finger protein 1; GC-box-binding zinc finger protein 1; GZP1; Transcription factor ZBP-99; ZBP 99; ZBP99; zfp281; Zinc finger DNA binding protein 99; Zinc finger DNA-binding protein 99; Zinc finger protein 281; ZN281_HUMAN; ZNF281; ZNP 99; ZNP 99 transcription factor; ZNP99;

Immunogens

Immunogen:
Uniprot:

Q9Y2X9(ZN281_HUMAN) >>Visit HPA database.

Gene(ID):
ZNF281(23528)
Sequence:
MKIGSGFLSGGGGTGSSGGSGSGGGGSGGGGGGGSSGRRAEMEPTFPQGMVMFNHRLPPVTSFTRPAGSAAPPPQCVLSSSTSAAPAAEPPPPPAPDMTFKKEPAASAAAFPSQRTSWGFLQSLVSIKQEKPADPEEQQSHHHHHHHHYGGLFAGAEERSPGLGGGEGGSHGVIQDLSILHQHVQQQPAQHHRDVLLSSSSRTDDHHGTEEPKQDTNVKKAKRPKPESQGIKAKRKPSASSKPSLVGDGEGAILSPSQKPHICDHCSAAFRSSYHLRRHVLIHTGERPFQCSQCSMGFIQKYLLQRHEKIHSREKPFGCDQCSMKFIQKYHMERHKRTHSGEKPYKCDTCQQYFSRTDRLLKHRRTCGEVIVKGATSAEPGSSNHTNMGNLAVLSQGNTSSSRRKTKSKSIAIENKEQKTGKTNESQISNNINMQSYSVEMPTVSSSGGIIGTGIDELQKRVPKLIFKKGSRKNTDKNYLNFVSPLPDIVGQKSLSGKPSGSLGIVSNNSVETIGLLQSTSGKQGQISSNYDDAMQFSKKRRYLPTASSNSAFSINVGHMVSQQSVIQSAGVSVLDNEAPLSLIDSSALNAEIKSCHDKSGIPDEVLQSILDQYSNKSESQKEDPFNIAEPRVDLHTSGEHSELVQEENLSPGTQTPSNDKASMLQEYSKYLQQAFEKSTNASFTLGHGFQFVSLSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2X9 As Substrate

Site PTM Type Enzyme
K2 Sumoylation
S16 Phosphorylation
K102 Ubiquitination
S117 Phosphorylation
K128 Sumoylation
S160 Phosphorylation
S198 Phosphorylation
S200 Phosphorylation
S201 Phosphorylation
T203 Phosphorylation
T209 Phosphorylation
K213 Sumoylation
K213 Ubiquitination
K225 Sumoylation
K225 Ubiquitination
K232 Sumoylation
S255 Phosphorylation
K259 Sumoylation
T284 Phosphorylation
K315 Ubiquitination
K325 Sumoylation
K325 Ubiquitination
K329 Sumoylation
K329 Ubiquitination
K343 Ubiquitination
K346 Sumoylation
K346 Ubiquitination
Y353 Phosphorylation
K373 Sumoylation
K373 Ubiquitination
S395 Phosphorylation
K409 Sumoylation
K409 Ubiquitination
S410 Phosphorylation
K416 Sumoylation
K416 Ubiquitination
S438 Phosphorylation
S445 Phosphorylation
S446 Phosphorylation
K464 Sumoylation
K477 Sumoylation
K477 Ubiquitination
Y479 Phosphorylation
S484 Phosphorylation
K498 Sumoylation
K498 Ubiquitination
S529 Phosphorylation
Y531 Phosphorylation
S538 Phosphorylation
K539 Sumoylation
K539 Ubiquitination
S595 Phosphorylation
K599 Ubiquitination
S600 Phosphorylation
S615 Phosphorylation
K617 Ubiquitination
S620 Phosphorylation
K622 Sumoylation
K622 Ubiquitination
T637 Phosphorylation
S638 Phosphorylation
S642 Phosphorylation
S651 Phosphorylation
T654 Phosphorylation
T656 Phosphorylation
K661 Ubiquitination
K670 Sumoylation
K670 Ubiquitination
S683 Phosphorylation
S697 Phosphorylation
S783 Phosphorylation
S785 Phosphorylation
K787 Sumoylation
K787 Ubiquitination
K792 Acetylation
K795 Sumoylation
K795 Ubiquitination
S800 Phosphorylation
S807 Phosphorylation
S812 Phosphorylation
K818 Ubiquitination
S837 Phosphorylation
K840 Sumoylation
K840 Ubiquitination
S864 Phosphorylation
T872 Phosphorylation
K887 Ubiquitination
T888 Phosphorylation
S891 O-Glycosylation

Research Backgrounds

Function:

Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

Restrictive clause

 

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For Research Use Only.
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