CCDC34 Antibody - #DF13697
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Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
CCDC34 Antibody detects endogenous levels of total CCDC34.
RRID:
AB_2846716
Cite Format: Affinity Biosciences Cat# DF13697, RRID:AB_2846716.
Cite Format: Affinity Biosciences Cat# DF13697, RRID:AB_2846716.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
CCD34_HUMAN; Ccdc34; Coiled-coil domain-containing protein 34; L15; NY-REN-41; RAMA3; Renal carcinoma antigen NY-REN-41;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q96HJ3 CCD34_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MWAAGRWGPTFPSSYAGFSADCRPRSRPSSDSCSVPMTGARGQGLEVVRSPSPPLPLSCSNSTRSLLSPLGHQSFQFDEDDGDGEDEEDVDDEEDVDEDAHDSEAKVASLRGMELQGCASTQVESENNQEEQKQVRLPESRLTPWEVWFIGKEKEERDRLQLKALEELNQQLEKRKEMEEREKRKIIAEEKHKEWVQKKNEQKRKEREQKINKEMEEKAAKELEKEYLQEKAKEKYQEWLKKKNAEECERKKKEKEKEKQQQAEIQEKKEIAEKKFQEWLENAKHKPRPAAKSYGYANGKLTGFYSGNSYPEPAFYNPIPWKPIHMPPPKEAKDLSGRKSKRPVISQPHKSSSLVIHKARSNLCLGTLCRIQR
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
100
Dog
88
Pig
71
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96HJ3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R41 | Methylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K259 | Acetylation | Uniprot | |
| K268 | Acetylation | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K350 | Methylation | Uniprot | |
| S361 | Phosphorylation | Uniprot |
Research Backgrounds
References
1). RABL2A–CCDC34 Axis Promotes Sorafenib Resistance in Hepatocellular Carcinoma. DNA and Cell Biology, 2021
(PubMed: 34767735)
[IF=2.6]
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