SPRED2 Antibody - #DF13707
| Product: | SPRED2 Antibody |
| Catalog: | DF13707 |
| Description: | Rabbit polyclonal antibody to SPRED2 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 47kDa; 48kD(Calculated). |
| Uniprot: | Q7Z698 |
| RRID: | AB_2846726 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13707, RRID:AB_2846726.
Fold/Unfold
C79158; FLJ21897; FLJ31917; MGC163164; Spred 2; Sprouty protein with EVH 1 domain 2 related sequence; sprouty related EVH1 domain containing 2; Sprouty related protein with EVH 1 domain 2; zgc:77284;
Immunogens
- Q7Z698 SPRE2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTIHNEAELGDDDVFTTATDSSSNSSQKREQPTRTISSPTSCEHRRIYTLGHLHDSYPTDHYHLDQPMPRPYRQVSFPDDDEEIVRINPREKIWMTGYEDYRHAPVRGKYPDPSEDADSSYVRFAKGEVPKHDYNYPYVDSSDFGLGEDPKGRGGSVIKTQPSRGKSRRRKEDGERSRCVYCRDMFNHEENRRGHCQDAPDSVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACYHCGVMCRCCGGKHKAAA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q7Z698 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S104 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| Y178 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| Y228 | Phosphorylation | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| Y251 | Phosphorylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| Y264 | Phosphorylation | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| Y268 | Phosphorylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S297 | Phosphorylation | Uniprot |
Research Backgrounds
Negatively regulates Ras signaling pathways and downstream activation of MAP kinases.
Phosphorylated on serine and threonine residues. Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination.
Ubiquitinated; leading to degradation by the proteasome.
Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>Secretory vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm.
Note: Detected in the cytoplasm of the stratum spinosum cells, where it is associated with cytoplasmic vesicles that are supposed to be secretory granules.
Expressed in liver, skin, small intestine, salivary gland and prostate.
Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers. Interacts with Ras (By similarity). May interact with ZDHHC13 (via ANK repeats) and ZDHHC17 (via ANK repeats) (By similarity).
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