FHOD1 Antibody - #DF13716
| Product: | FHOD1 Antibody |
| Catalog: | DF13716 |
| Description: | Rabbit polyclonal antibody to FHOD1 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 126kDa; 127kD(Calculated). |
| Uniprot: | Q9Y613 |
| RRID: | AB_2846735 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13716, RRID:AB_2846735.
Fold/Unfold
FH1/FH2 domain containing protein; FH1/FH2 domain containing protein 1; FH1/FH2 domain-containing protein 1; Fhod1; FHOD1_HUMAN; FHOS; FHOS1; Formin homolog overexpressed in spleen 1; Formin homology 2 domain containing 1; Formin homology 2 domain containing protein 1; Formin homology 2 domain-containing protein 1;
Immunogens
- Q9Y613 FHOD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGGEDRGDGEPVSVVTVRVQYLEDTDPFACANFPEPRRAPTCSLDGALPLGAQIPAVHRLLGAPLKLEDCALQVSPSGYYLDTELSLEEQREMLEGFYEEISKGRKPTLILRTQLSVRVNAILEKLYSSSGPELRRSLFSLKQIFQEDKDLVPEFVHSEGLSCLIRVGAAADHNYQSYILRALGQLMLFVDGMLGVVAHSDTIQWLYTLCASLSRLVVKTALKLLLVFVEYSENNAPLFIRAVNSVASTTGAPPWANLVSILEEKNGADPELLVYTVTLINKTLAALPDQDSFYDVTDALEQQGMEALVQRHLGTAGTDVDLRTQLVLYENALKLEDGDIEEAPGAGGRRERRKPSSEEGKRSRRSLEGGGCPARAPEPGPTGPASPVGPTSSTGPALLTGPASSPVGPPSGLQASVNLFPTISVAPSADTSSERSIYKARFLENVAAAETEKQVALAQGRAETLAGAMPNEAGGHPDARQLWDSPETAPAARTPQSPAPCVLLRAQRSLAPEPKEPLIPASPKAEPIWELPTRAPRLSIGDLDFSDLGEDEDQDMLNVESVEAGKDIPAPSPPLPLLSGVPPPPPLPPPPPIKGPFPPPPPLPLAAPLPHSVPDSSALPTKRKTVKLFWRELKLAGGHGVSASRFGPCATLWASLDPVSVDTARLEHLFESRAKEVLPSKKAGEGRRTMTTVLDPKRSNAINIGLTTLPPVHVIKAALLNFDEFAVSKDGIEKLLTMMPTEEERQKIEEAQLANPDIPLGPAENFLMTLASIGGLAARLQLWAFKLDYDSMEREIAEPLFDLKVGMEQLVQNATFRCILATLLAVGNFLNGSQSSGFELSYLEKVSEVKDTVRRQSLLHHLCSLVLQTRPESSDLYSEIPALTRCAKVDFEQLTENLGQLERRSRAAEESLRSLAKHELAPALRARLTHFLDQCARRVAMLRIVHRRVCNRFHAFLLYLGYTPQAAREVRIMQFCHTLREFALEYRTCRERVLQQQQKQATYRERNKTRGRMITETEKFSGVAGEAPSNPSVPVAVSSGPGRGDADSHASMKSLLTSRPEDTTHNRRSRGMVQSSSPIMPTVGPSTASPEEPPGSSLPSDTSDEIMDLLVQSVTKSSPRALAARERKRSRGNRKSLRRTLKSGLGDDLVQALGLSKGPGLEV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y613 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| C31 | S-Nitrosylation | Uniprot | |
| Y99 | Phosphorylation | P12931 (SRC) | Uniprot |
| K126 | Ubiquitination | Uniprot | |
| Y176 | Phosphorylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| Y439 | Phosphorylation | Uniprot | |
| T465 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| T489 | Phosphorylation | Uniprot | |
| T495 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| S523 | Phosphorylation | Uniprot | |
| K525 | Ubiquitination | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| S573 | Phosphorylation | Uniprot | |
| K635 | Methylation | Uniprot | |
| K676 | Ubiquitination | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| T690 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| T708 | Phosphorylation | Uniprot | |
| K730 | Ubiquitination | Uniprot | |
| T816 | Phosphorylation | Uniprot | |
| T823 | Phosphorylation | Uniprot | |
| K851 | Ubiquitination | Uniprot | |
| S874 | Phosphorylation | Uniprot | |
| Y878 | Phosphorylation | Uniprot | |
| K889 | Ubiquitination | Uniprot | |
| K918 | Ubiquitination | Uniprot | |
| Y960 | Phosphorylation | Uniprot | |
| Y963 | Phosphorylation | Uniprot | |
| K1000 | Ubiquitination | Uniprot | |
| T1010 | Phosphorylation | Uniprot | |
| R1044 | Methylation | Uniprot | |
| T1103 | Phosphorylation | Uniprot | |
| S1114 | Phosphorylation | Uniprot | |
| S1131 | Phosphorylation | Q13464 (ROCK1) , Q13976 (PRKG1) | Uniprot |
| S1137 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T1141 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| S1144 | Phosphorylation | Uniprot |
Research Backgrounds
Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Acts synergistically with ROCK1 to promote SRC-dependent non-apoptotic plasma membrane blebbing.
Phosphorylated by ROCK1.
Cytoplasm. Cytoplasm>Cytoskeleton. Cell projection>Bleb.
Note: Predominantly cytoplasmic.
Ubiquitous. Highly expressed in spleen.
Self-associates via the FH2 domain. Binds to F-actin via its N-terminus. Binds to the cytoplasmic domain of CD21 via its C-terminus. Interacts with ROCK1 in a Src-dependent manner.
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.
Belongs to the formin homology family.
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