TEX14 Antibody | Affinity Biosciences

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TEX14 Antibody - Western blot analysis of extracts from HepG2 cells, using TEX14 Antibody.

Product:	TEX14 Antibody
Catalog:	DF13723
Description:	Rabbit polyclonal antibody to TEX14
Application:	WB
Reactivity:	Human
Mol.Wt.:	167kDa; 168kD(Calculated).
Uniprot:	Q8IWB6
RRID:	AB_2846742

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

TEX14 Antibody detects endogenous levels of total TEX14.

RRID:

AB_2846742
Cite Format: Affinity Biosciences Cat# DF13723, RRID:AB_2846742.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cancer/testis antigen 113; CT113; Inactive serine/threonine protein kinase TEX14; Protein kinase like protein SgK307; Sugen kinase 307; Testis expressed Protein 14; Testis expressed sequence 14; Testis expressed sequence 14 protein;

Immunogens

Immunogen:

Uniprot:

Q8IWB6(TEX14_HUMAN) >>Visit HPA database.

Gene(ID):

TEX14(56155)

Expression:

Q8IWB6 TEX14_HUMAN:

Expression restricted to testis.

Sequence:

Q8IWB6 TEX14_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRAVRLPVPCPVQLGTLRNDSLEAQLHEYVKQGNYVKVKKILKKGIYVDAVNSLGQTALFVAALLGLRKFVDVLVDYGSDPNHRCFDGSTPVHAAAFSGNQWILSKLLDAGGDLRLHDERGQNPKTWALTAGKERSTQIVEFMQRCASHMQAIIQGFSYDLLKKIDSPQRLVYSPSWCGGLVQGNPNGSPNRLLKAGVISAQNIYSFGFGKAMPWFQFYLTGATQMAYLGSLPVIGEKEVIQADDEPTFSFFSGPYMVMTNLVWNGSRVTVKELNLPTHPHCSRLRLADLLIAEQEHSSKLRHPYLLQLMAVCLSQDLEKTRLVYERITIGTLFSVLHERRSQFPVLHMEVIVHLLLQISDALRYLHFQGFIHRSLSSYAVHIISPGEARLTNLEYMLESEDRGVQRDLTRVPLPTQLYNWAAPEVILQKAATVKSDIYSFSMIMQEILTDDIPWKGLDGSVVKKAVVSGNYLEADVRLPKPYYDIVKSGIHVKQKDRTMNLQDIRYILKNDLKDFTGAQRTQPTESPRVQRYGLHPDVNVYLGLTSEHPRETPDMEIIELKEMGSQPHSPRVHSLFTEGTLDPQAPDPCLMARETQNQDAPCPAPFMAEEASSPSTGQPSLCSFEINEIYSGCLILEDDIEEPPGAASSLEADGPNQVDELKSMEEELDKMEREACCFGSEDESSSKAETEYSFDDWDWQNGSLSSLSLPESTREAKSNLNNMSTTEEYLISKCVLDLKIMQTIMHENDDRLRNIEQILDEVEMKQKEQEERMSLWATSREFTNAYKLPLAVGPPSLNYIPPVLQLSGGQKPDTSGNYPTLPRFPRMLPTLCDPGKQNTDEQFQCTQGAKDSLETSRIQNTSSQGRPRESTAQAKATQFNSALFTLSSHRQGPSASPSCHWDSTRMSVEPVSSEIYNAESRNKDDGKVHLKWKMEVKEMAKKAATGQLTVPPWHPQSSLTLESEAENEPDALLQPPIRSPENTDWQRVIEYHRENDEPRGNGKFDKTGNNDCDSDQHGRQPRLGSFTSIRHPSPRQKEQPEHSEAFQASSDTLVAVEKSYSHQSMQSTCSPESSEDITDEFLTPDGEYFYSSTAQENLALETSSPIEEDFEGIQGAFAQPQVSGEEKFQMRKILGKNAEILPRSQFQPVRSTEDEQEETSKESPKELKEKDISLTDIQDLSSISYEPDSSFKEASCKTPKINHAPTSVSTPLSPGSVSSAASQYKDCLESITFQVKTEFASCWNSQEFIQTLSDDFISVRERAKKLDSLLTSSETPPSRLTGLKRLSSFIGAGSPSLVKACDSSPPHATQRRSLPKVEAFSQHHIDELPPPSQELLDDIELLKQQQGSSTVLHENTASDGGGTANDQRHLEEQETDSKKEDSSMLLSKETEDLGEDTERAHSTLDEDLERWLQPPEESVELQDLPKGSERETNIKDQKVGEEKRKREDSITPERRKSEGVLGTSEEDELKSCFWKRLGWSESSRIIVLDQSDLSD

PTMs - Q8IWB6 As Substrate

Q8IWB6

Site	PTM Type	Enzyme	Source
Y36	Phosphorylation		Uniprot
Y206	Phosphorylation		Uniprot
S284	Phosphorylation		Uniprot
S437	Phosphorylation	P53350 (PLK1)	Uniprot
Y508	Phosphorylation		Uniprot
K511	Acetylation		Uniprot
K515	Acetylation		Uniprot
T618	Phosphorylation	P06493 (CDK1)	Uniprot
T727	Phosphorylation	P06493 (CDK1)	Uniprot
T728	Phosphorylation	P06493 (CDK1)	Uniprot
K877	Acetylation		Uniprot
S915	Phosphorylation		Uniprot
K925	Acetylation		Uniprot
K935	Acetylation		Uniprot
K935	Sumoylation		Uniprot
Y1187	Phosphorylation		Uniprot
S1215	Phosphorylation		Uniprot
Y1226	Phosphorylation		Uniprot
T1277	Phosphorylation		Uniprot
S1280	Phosphorylation		Uniprot
S1305	Phosphorylation		Uniprot
S1384	Phosphorylation		Uniprot
S1385	Phosphorylation		Uniprot

Research Backgrounds

Q8IWB6_TEX14_HUMAN

Function:

Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro (By similarity).

PTMs:

Phosphorylated on Thr residues by CDK1 during early phases of mitosis, promoting the interaction with PLK1 and recruitment to kinetochores. Phosphorylated on Ser-437 by PLK1 during late prometaphase promotes the rapid depletion from kinetochores and its subsequent degradation by the APC/C complex.

Subcellular Location:

Cytoplasm. Midbody. Chromosome>Centromere>Kinetochore.
Note: Detected in the intercellular bridges that connect male germ cell daughter cells after cell division.

Tissue Specificity:

Expression restricted to testis.

Subunit Structure:

Interacts with KIF23 and RBM44. Interacts with CEP55; inhibiting interaction between CEP55 and PDCD6IP/ALIX and TSG101 (By similarity).

Family&Domains:

The protein kinase domain is predicted to be catalytically inactive.

The GPPX3Y motif mediates interaction with CEP55.

Belongs to the protein kinase superfamily.

Restrictive clause

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For Research Use Only.
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TEX14 Antibody - #DF13723