FKBP135 Antibody | Affinity Biosciences

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FKBP135 Antibody - Western blot analysis of extracts from Hela cells, using FKBP135 Antibody.

Product:	FKBP135 Antibody
Catalog:	DF13737
Description:	Rabbit polyclonal antibody to FKBP135
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep
Mol.Wt.:	133kDa; 134kD(Calculated).
Uniprot:	Q5T1M5
RRID:	AB_2846756

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(88%), Sheep(88%)

Clonality:

Polyclonal

Specificity:

FKBP135 Antibody detects endogenous levels of total FKBP135.

RRID:

AB_2846756
Cite Format: Affinity Biosciences Cat# DF13737, RRID:AB_2846756.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

Q5T1M5(FKB15_HUMAN) >>Visit HPA database.

Gene(ID):

FKBP15(23307)

Sequence:

Q5T1M5 FKB15_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFGAGDEDDTDFLSPSGGARLASLFGLDQAAAGHGNEFFQYTAPKQPKKGQGTAATGNQATPKTAPATMSTPTILVATAVHAYRYTNGQYVKQGKFGAAVLGNHTAREYRILLYISQQQPVTVARIHVNFELMVRPNNYSTFYDDQRQNWSIMFESEKAAVEFNKQVCIAKCNSTSSLDAVLSQDLIVADGPAVEVGDSLEVAYTGWLFQNHVLGQVFDSTANKDKLLRLKLGSGKVIKGWEDGMLGMKKGGKRLLIVPPACAVGSEGVIGWTQATDSILVFEVEVRRVKFARDSGSDGHSVSSRDSAAPSPIPGADNLSADPVVSPPTSIPFKSGEPALRTKSNSLSEQLAINTSPDAVKAKLISRMAKMGQPMLPILPPQLDSNDSEIEDVNTLQGGGQPVVTPSVQPSLHPAHPALPQMTSQAPQPSVTGLQAPSAALMQVSSLDSHSAVSGNAQSFQPYAGMQAYAYPQASAVTSQLQPVRPLYPAPLSQPPHFQGSGDMASFLMTEARQHNTEIRMAVSKVADKMDHLMTKVEELQKHSAGNSMLIPSMSVTMETSMIMSNIQRIIQENERLKQEILEKSNRIEEQNDKISELIERNQRYVEQSNLMMEKRNNSLQTATENTQARVLHAEQEKAKVTEELAAATAQVSHLQLKMTAHQKKETELQMQLTESLKETDLLRGQLTKVQAKLSELQETSEQAQSKFKSEKQNRKQLELKVTSLEEELTDLRVEKESLEKNLSERKKKSAQERSQAEEEIDEIRKSYQEELDKLRQLLKKTRVSTDQAAAEQLSLVQAELQTQWEAKCEHLLASAKDEHLQQYQEVCAQRDAYQQKLVQLQEKCLALQAQITALTKQNEQHIKELEKNKSQMSGVEAAASDPSEKVKKIMNQVFQSLRREFELEESYNGRTILGTIMNTIKMVTLQLLNQQEQEKEESSSEEEEEKAEERPRRPSQEQSASASSGQPQAPLNRERPESPMVPSEQVVEEAVPLPPQALTTSQDGHRRKGDSEAEALSEIKDGSLPPELSCIPSHRVLGPPTSIPPEPLGPVSMDSECEESLAASPMAAKPDNPSGKVCVREVAPDGPLQESSTRLSLTSDPEEGDPLALGPESPGEPQPPQLKKDDVTSSTGPHKELSSTEAGSTVAGAALRPSHHSQRSSLSGDEEDELFKGATLKALRPKAQPEEEDEDEVSMKGRPPPTPLFGDDDDDDDIDWLG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Pig

Dog

Rabbit

Horse

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5T1M5 As Substrate

Q5T1M5

Site	PTM Type	Source
M1	Acetylation	Uniprot
S14	Phosphorylation	Uniprot
S23	Phosphorylation	Uniprot
Y41	Phosphorylation	Uniprot
T42	Phosphorylation	Uniprot
T61	Phosphorylation	Uniprot
K92	Acetylation	Uniprot
S295	Phosphorylation	Uniprot
S301	Phosphorylation	Uniprot
S303	Phosphorylation	Uniprot
S307	Phosphorylation	Uniprot
S311	Phosphorylation	Uniprot
S320	Phosphorylation	Uniprot
S326	Phosphorylation	Uniprot
T329	Phosphorylation	Uniprot
S330	Phosphorylation	Uniprot
T342	Phosphorylation	Uniprot
S344	Phosphorylation	Uniprot
S346	Phosphorylation	Uniprot
S348	Phosphorylation	Uniprot
S356	Phosphorylation	Uniprot
R520	Methylation	Uniprot
S555	Phosphorylation	Uniprot
K594	Ubiquitination	Uniprot
S619	Phosphorylation	Uniprot
T660	Phosphorylation	Uniprot
S767	Phosphorylation	Uniprot
K774	Ubiquitination	Uniprot
S795	Phosphorylation	Uniprot
K808	Ubiquitination	Uniprot
K837	Ubiquitination	Uniprot
S874	Phosphorylation	Uniprot
S939	Phosphorylation	Uniprot
S940	Phosphorylation	Uniprot
S941	Phosphorylation	Uniprot
S956	Phosphorylation	Uniprot
S960	Phosphorylation	Uniprot
S962	Phosphorylation	Uniprot
S964	Phosphorylation	Uniprot
S965	Phosphorylation	Uniprot
S979	Phosphorylation	Uniprot
S984	Phosphorylation	Uniprot
S1012	Phosphorylation	Uniprot
S1018	Phosphorylation	Uniprot
S1024	Phosphorylation	Uniprot
S1034	Phosphorylation	Uniprot
S1061	Phosphorylation	Uniprot
S1065	Phosphorylation	Uniprot
S1092	Phosphorylation	Uniprot
S1093	Phosphorylation	Uniprot
T1094	Phosphorylation	Uniprot
S1097	Phosphorylation	Uniprot
T1099	Phosphorylation	Uniprot
S1100	Phosphorylation	Uniprot
S1114	Phosphorylation	Uniprot
S1158	Phosphorylation	Uniprot
S1161	Phosphorylation	Uniprot
S1162	Phosphorylation	Uniprot
S1164	Phosphorylation	Uniprot
S1195	Phosphorylation	Uniprot
T1203	Phosphorylation	Uniprot

Research Backgrounds

Q5T1M5_FKB15_HUMAN

Function:

May be involved in the cytoskeletal organization of neuronal growth cones. Seems to be inactive as a PPIase (By similarity). Involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement.

Subcellular Location:

Cytoplasm. Cell projection>Axon. Early endosome.
Note: Present in axons and neuronal growth cones.

Subunit Structure:

Interacts with WIP and actin. Interacts with TBC1D23.

Family&Domains:

The PPIase FKBP-type domain seems to be inactive both for FK506-binding and enzymatic activity.

The central coiled-coil region is responsible for association with early endosomes.

Belongs to the FKBP-type PPIase family.

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FKBP135 Antibody - #DF13737