SMC5 Antibody - #DF13744

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Product: SMC5 Antibody
Catalog: DF13744
Description: Rabbit polyclonal antibody to SMC5
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 128kDa; 129kD(Calculated).
Uniprot: Q8IY18
RRID: AB_2846763

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SMC5 Antibody detects endogenous levels of total SMC5.
RRID:
AB_2846763
Cite Format: Affinity Biosciences Cat# DF13744, RRID:AB_2846763.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hSMC 5; hSMC5; KIAA0594; MLZ-453; OTTHUMP00000063367; SMC 5; SMC 5 structural maintenance of chromosomes 5 like 1; SMC protein 5; SMC5 L1; SMC5 protein; SMC5 structural maintenance of chromosomes 5 like 1; SMC5L1; Structural maintenance of chromosomes 5; Structural maintenance of chromosomes 5 like 1; Structural maintenance of chromosomes protein 5;

Immunogens

Immunogen:
Uniprot:

Q8IY18(SMC5_HUMAN) >>Visit HPA database.

Gene(ID):
SMC5(23137)
Expression:
Q8IY18 SMC5_HUMAN:

Widely expressed (PubMed:11408570). Strongly expressed in testis (PubMed:11408570).

Sequence:
MATPSKKTSTPSPQPSKRALPRDPSSEVPSKRKNSAPQLPLLQSSGPFVEGSIVRISMENFLTYDICEVSPGPHLNMIVGANGTGKSSIVCAICLGLAGKPAFMGRADKVGFFVKRGCSRGMVEIELFRASGNLVITREIDVAKNQSFWFINKKSTTQKIVEEKVAALNIQVGNLCQFLPQDKVGEFAKLSKIELLEATEKSIGPPEMHKYHCELKNLREKEKQLETSCKEKTEYLQKMVQRNERYKQDVERFYERKRHLDLIEMLEAKRPWVEYENVRQEYEEVKLVRDRVKEEVRKLKEGQIPVTCRIEEMENERHNLEARIKEKATDIKEASQKCKQKQDVIERKDKHIEELQQALIVKQNEELDRQRRIGNTRKMIEDLQNELKTTENCENLQPQIDAITNDLRRIQDEKALCEGEIIDKRRERETLEKEKKSVDDHIVRFDNLMNQKEDKLRQRFRDTYDAVLWLRNNRDKFKQRVCEPIMLTINMKDNKNAKYIENHIPSNDLRAFVFESQEDMEVFLKEVRDNKKLRVNAVIAPKSSYADKAPSRSLNELKQYGFFSYLRELFDAPDPVMSYLCCQYHIHEVPVGTEKTRERIERVIQETRLKQIYTAEEKYVVKTSFYSNKVISSNTSLKVAQFLTVTVDLEQRRHLEEQLKEIHRKLQAVDSGLIALRETSKHLEHKDNELRQKKKELLERKTKKRQLEQKISSKLGSLKLMEQDTCNLEEEERKASTKIKEINVQKAKLVTELTNLIKICTSLHIQKVDLILQNTTVISEKNKLESDYMAASSQLRLTEQHFIELDENRQRLLQKCKELMKRARQVCNLGAEQTLPQEYQTQVPTIPNGHNSSLPMVFQDLPNTLDEIDALLTEERSRASCFTGLNPTIVQEYTKREEEIEQLTEELKGKKVELDQYRENISQVKERWLNPLKELVEKINEKFSNFFSSMQCAGEVDLHTENEEDYDKYGIRIRVKFRSSTQLHELTPHHQSGGERSVSTMLYLMALQELNRCPFRVVDEINQGMDPINERRVFEMVVNTACKENTSQYFFITPKLLQNLPYSEKMTVLFVYNGPHMLEPNTWNLKAFQRRRRRITFTQPS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IY18 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
T10 Phosphorylation
S12 Phosphorylation
S16 Phosphorylation
S25 Phosphorylation
S26 Phosphorylation
K31 Ubiquitination
S35 Phosphorylation
S44 Phosphorylation
K100 Ubiquitination
K109 Acetylation
K109 Ubiquitination
K115 Acetylation
K115 Ubiquitination
T157 Phosphorylation
K159 Ubiquitination
K183 Ubiquitination
K189 Ubiquitination
K192 Ubiquitination
T199 Phosphorylation
K210 Ubiquitination
K216 Ubiquitination
T227 Phosphorylation
S228 Phosphorylation
K232 Ubiquitination
K238 Ubiquitination
K269 Ubiquitination
Y275 Phosphorylation
Y282 Phosphorylation
K286 Ubiquitination
K300 Ubiquitination
K350 Ubiquitination
K362 Ubiquitination
K378 Ubiquitination
K414 Ubiquitination
K424 Ubiquitination
K436 Ubiquitination
K452 Ubiquitination
K455 Ubiquitination
K498 Ubiquitination
S506 Phosphorylation
K542 Ubiquitination
K548 Acetylation
K548 Ubiquitination
K558 Ubiquitination
K595 Ubiquitination
K610 Ubiquitination
K618 Ubiquitination
Y619 Phosphorylation
Y626 Phosphorylation
K629 Ubiquitination
K665 Ubiquitination
K714 Ubiquitination
K719 Ubiquitination
K740 Ubiquitination
K748 Ubiquitination
T751 Phosphorylation
T754 Phosphorylation
S779 Phosphorylation
S792 Phosphorylation
S793 Phosphorylation
K895 Ubiquitination
K908 Ubiquitination
K911 Ubiquitination
K925 Ubiquitination
K933 Ubiquitination
T987 Phosphorylation
T1046 Phosphorylation
S1047 Phosphorylation
Y1062 Phosphorylation
T1096 Phosphorylation

Research Backgrounds

Function:

Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome.

PTMs:

Sumoylated.

Ubiquitinated.

(Microbial infection) SMC5-SMC6 complex is degraded by the activity of Hepatitis B X protein.

Subcellular Location:

Nucleus. Chromosome. Nucleus>PML body. Chromosome>Telomere.
Note: Associates with chromatin (PubMed:25931565). Colocalizes with SMC6 on the X-Y chromosome pair within the sex vesicle during late pachytene/diplotene (By similarity). Localizes to PML nuclear bodies in ALT cell lines (PubMed:17589526). Accumulates with RAD18 and the SLF1-SLF2 complex at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Strongly expressed in testis.

Subunit Structure:

Forms a heterodimer with SMC6. Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NSMCE3. Interacts with NSMCE2. Interacts with SLF2; this interaction induces an association of the SLF1-SLF2 complex with the SMC5-SMC6 complex. Interacts with RAD18; this interaction is increased in a SLF1 or SLF2-dependent manner.

(Microbial infection) SMC5-SMC6 complex interacts with Hepatitis B X protein.

Family&Domains:

The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC6, forming a V-shaped heterodimer.

Belongs to the SMC family. SMC5 subfamily.

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