Phospho-ZO-2 (Tyr1118) Antibody | Affinity Biosciences

1/2

Phospho-ZO-2 (Tyr1118) Antibody - Western blot analysis of extracts from HepG2 cells(heat-shock treatment), using Phospho-ZO-2 (Tyr1118) Antibody.

Phospho-ZO-2 (Tyr1118) Antibody - AF3518 at 1/100 staining Mouse testis tissue by IHC-P.

Product:	Phospho-ZO-2 (Tyr1118) Antibody
Catalog:	AF3518
Description:	Rabbit polyclonal antibody to Phospho-ZO-2 (Tyr1118)
Application:	WB IHC
Reactivity:	Human, Mouse
Mol.Wt.:	134kD(Calculated).
Uniprot:	Q9UDY2
RRID:	AB_2846832

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Phospho-ZO-2 (Tyr1118) Antibody detects endogenous levels of ZO-2 only when phosphorylated at Tyr1118.

RRID:

AB_2846832
Cite Format: Affinity Biosciences Cat# AF3518, RRID:AB_2846832.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C9DUPq21.11; DFNA51; DUP9q21.11; Friedreich ataxia region gene X104 (tight junction protein ZO-2); MGC26306; PFIC4; Tight junction protein 2; Tight junction protein ZO 2; Tight junction protein ZO-2; TJP2; X104; ZO 2; ZO-2; ZO2; ZO2_HUMAN; Zona occludens 2; Zona occludens protein 2; Zonula occludens protein 2;

Immunogens

Immunogen:

A synthesized peptide derived from human ZO-2 around the phosphorylation site of Tyr1118.

Uniprot:

Q9UDY2(ZO2_HUMAN) >>Visit HPA database.

Gene(ID):

TJP2(9414)

Expression:

Q9UDY2 ZO2_HUMAN:

This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.

Sequence:

Q9UDY2 ZO2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSRLNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRGRSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSRGPRSRSREHPHSRSPSPEPRGRPGPIGVLLMKSRANEEYGLRLGSQIFVKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSQQTLINIPSLNDSDSEIEDISEIESNRSFSPEERRHQYSDYDYHSSSEKLKERPSSREDTPSRLSRMGATPTPFKSTGDIAGTVVPETNKEPRYQEDPPAPQPKAAPRTFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGEMVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGNWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRDNAGDRADFWRMRGQRSGVKKNLRKSREDLTAVVSVSTKFPAYERVLLREAGFKRPVVLFGPIADIAMEKLANELPDWFQTAKTEPKDAGSEKSTGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTMRQRLNPTSNKSSRKLFDQANKLKKTCAHLFTATINLNSANDSWFGSLKDTIQHQQGEAVWVSEGKMEGMDDDPEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASSDQLRDNSPPPAFKPEPPKAKTQNKEESYDFSKSYEYKSNPSAVAGNETPGASTKGYPPPVAAKPTFGRSILKPSTPIPPQEGEEVGESSEEQDNAPKSVLGKVKIFEKMDHKARLQRMQELQEAQNARIEIAQKHPDIYAVPIKTHKPDPGTPQHTSSRPPEPQKAPSRPYQDTRGSYGSDAEEEEYRQQLSEHSKRGYYGQSARYRDTEL

Research Backgrounds

Q9UDY2_ZO2_HUMAN

Function:

Plays a role in tight junctions and adherens junctions.

Subcellular Location:

Cell junction>Adherens junction. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Tight junction. Nucleus.
Note: Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.

Tissue Specificity:

Family&Domains:

Belongs to the MAGUK family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-ZO-2 (Tyr1118) Antibody - #AF3518