Phospho-SRPK2 (Ser494/Ser497) Antibody - #AF3633

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Product: Phospho-SRPK2 (Ser494/Ser497) Antibody
Catalog: AF3633
Description: Rabbit polyclonal antibody to Phospho-SRPK2 (Ser494/Ser497)
Application: IHC
Cited expt.:
Reactivity: Human, Mouse, Rat
Mol.Wt.: 78kD,110~130kD; 78kD(Calculated).
Uniprot: P78362
RRID: AB_2846947

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-SRPK2 (Ser494/Ser497) Antibody detects endogenous levels of SRPK2 only when phosphorylated at Ser494/497.
RRID:
AB_2846947
Cite Format: Affinity Biosciences Cat# AF3633, RRID:AB_2846947.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Human serine kinase SRPK2 mRNA, complete cds; Serine kinase SRPK2; Serine/arginine rich protein specific kinase 2; Serine/arginine-rich protein-specific kinase 2; Serine/threonine protein kinase SRPK2; SFRS protein kinase 2; SFRSK2; SR protein specific kinase 2; SR-protein-specific kinase 2; SRPK2; SRPK2_HUMAN; SRSF protein kinase 2 C-terminal;

Immunogens

Immunogen:

A synthesized peptide derived from human SRPK2 around the phosphorylation site of Ser494/497.

Uniprot:

P78362(SRPK2_HUMAN) >>Visit HPA database.

Gene(ID):
SRPK2(6733)
Expression:
P78362 SRPK2_HUMAN:

Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.

Sequence:
MSVNSEKSSSSERPEPQQKAPLVPPPPPPPPPPPPPLPDPTPPEPEEEILGSDDEEQEDPADYCKGGYHPVKIGDLFNGRYHVIRKLGWGHFSTVWLCWDMQGKRFVAMKVVKSAQHYTETALDEIKLLKCVRESDPSDPNKDMVVQLIDDFKISGMNGIHVCMVFEVLGHHLLKWIIKSNYQGLPVRCVKSIIRQVLQGLDYLHSKCKIIHTDIKPENILMCVDDAYVRRMAAEATEWQKAGAPPPSGSAVSTAPQQKPIGKISKNKKKKLKKKQKRQAELLEKRLQEIEELEREAERKIIEENITSAAPSNDQDGEYCPEVKLKTTGLEEAAEAETAKDNGEAEDQEEKEDAEKENIEKDEDDVDQELANIDPTWIESPKTNGHIENGPFSLEQQLDDEDDDEEDCPNPEEYNLDEPNAESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRSRTVSASSTGDLPKAKTRAADLLVNPLDPRNADKIRVKIADLGNACWVHKHFTEDIQTRQYRSIEVLIGAGYSTPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIIELLGSIPRHFALSGKYSREFFNRRGELRHITKLKPWSLFDVLVEKYGWPHEDAAQFTDFLIPMLEMVPEKRASAGECLRHPWLNS

Research Backgrounds

Function:

Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.

PTMs:

Phosphorylation at Thr-492 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage.

Proteolytically cleaved at Asp-139 and Asp-403 by caspase-3 during apoptotic cell death. Cleavage at Asp-139 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the cytoplasm. KAT5/TIP60 inhibits its nuclear translocation. Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.

References

1). Host Tau Genotype Specifically Designs and Regulates Tau Seeding and Spreading and Host Tau Transformation Following Intrahippocampal Injection of Identical Tau AD Inoculum. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022 (PubMed: 35054902) [IF=5.6]

Application: IF/ICC    Species: Mice    Sample:

Figure 7 Double-labeling immunofluorescence and confocal microscopy to p38-P Thr180/Tyr182 or SAPK/JNK-PThr183/Thr185 (green), and AT8 (red) in inoculated hTau at the age of six months and killed at the age of nine months shows co-localization of the active kinases with phospho-tau deposition (white arrows) (A and B, respectively for p38-P and SAPK-JNK-P) in dentate gyrus neurons. Double-labelling immunofluorescence and confocal microscopy to PKAα/β-P Tyr197 and AT8 in inoculated hTau mice shows cytoplasmic granular PKAα/β-P Tyr197 granules in CA1 neurons with abnormal phospho-tau deposits in neurons (C, arrow). Cytoplasmic SRPK2-Ser497 immunoreactivity is found in neurons with phospho-tau deposits in inoculated hTau mice (D). Paraffin sections, nuclei are stained with DRAQ5™ (blue), A, B, bar = 10 µm, C and D, bar = 20 µm. CLK1 immunoreactivity in the CA1 region of non-inoculated (E) and inoculated hTau with AD sarkosyl-insoluble fractions (F); small cytoplasmic granules are seen in inoculated hTau. Paraffin sections, slightly counterstained with haematoxylin, bar = 40 µm.
2). Dysregulated Protein Phosphorylation in a Mouse Model of FTLD-Tau. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 2022 (PubMed: 35848963) [IF=3.2]
3). Dysregulated protein phosphorylation in tauopathy. Research Square, 2022

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